NEUTROPHIL FUNCTIONAL TESTS
Bactericidal Assays and Chemotaxis
NJH-ADL
Bactericidal assay. Analysis of the ability of isolated neutrophils to clear S. aureus – manual method.
Test code(s): BACT
Neutrophil chemotaxis. Modified Boyden chamber method utilizing peripheral blood and zymosan activated serum as chemotactic agent. Readout is distance migrated in microns.
Test code(s): CTX
DHR/Oxidative Burst
ARUP
Neutrophil Oxidative Burst Assay (DHR). White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by Semi-Quantitative Flow Cytometry.
Test code(s): 0096657
BCH-CFCL
Neutrophil’s Oxidative Burst, which reports: Neutrophil Oxidative Index (MFI DHR+ / MFI DHR-), and % Responding Polymorphonuclear Cells.
Test code(s): DHR assay
CALGARY-CLS
Functional CGD screening assay, using DHR123 loaded neutrophils pre and post stimulation with PMA to induce oxidative burst reaction.
Test code(s): NFUN
Functional CGD screening assay, using DHR123 loaded neutrophils pre and post stimulation with PMA to induce oxidative burst reaction.
Test code(s): NFUN
CCHMC-DIL
Classic dihydrorhodamine (DHR) 123 assay.
Test code(s): Neutrophil Oxidative Burst (DHR)
CHLA-DIFL
Chronic granulomatous disease diagnostic screening assay for oxidative burst using dihydrorhodamine (DHR) 123.
Test code(s): CGD Flow
IOWA
Classic dihydrorhodamine (DHR) 123 assay with PMA stimulation.
Test code(s): SPL8
MAYO-ML
Classic dihydrorhodamine (DHR) 123 assay.
Test code(s): DHR (for both PMA and fMLP stimulants); #62764; DHRP (only for PMA); #62765; DHRF (only for fMLP); #62766.
MONTREAL-SJH
Neutrophil’s oxidation (DHR) by flow cytometry.
Test code(s): Activité Oxidative des Neutrophiles
NJH-ADL
Classic dihydrorhodamine (DHR) 123 assay. Offers NBT assay as back up.
Test code(s): DHR; NBT
NSUH
Neutrophil oxidation assay uses dihydrorhodamine (DHR) 123 and PMA. Report includes MFI ratio.
Test code(s): DHR
QUEST
Neutrophil Function, Oxidative Burst. Classic dihydrorhodamine (DHR) 123 assay.
Test code(s): 37523
UCLA-UIC
Classic dihydrorhodamine (DHR) 123 assay with PMA stimulation.
Test code(s): GOB
VIRACOR
Oxidative Burst: Whole blood is stimulated with PMA or buffer alone, Formation of the reactive oxidants during the oxidative burst is monitored by the addition and enzymatic oxidation of a fluorogenic substrate, DHR 123. The level of reactive oxygen radicals is determined by flow cytometry.
Test code(s): 403002
