NK CELL FUNCTIONAL TESTS
CALGARY-CLS
Evaluation of patient NK cell CD107a surface expression following exposure to K562 cells.
Test code(s): CD107A
CCHMC-DIL
Evaluation of CD107a surface expression/degranulation following exposure of NK cells to K562 cells.
Test code(s): CD107a Mobilization (NK Cell Degran)
Spontaneous NK cell cytotoxicity against K562 cells (4h stimulation, Cr51 assay).
Test code(s): NK Function
IOWA
Spontaneous NK cell cytotoxicity against K562 cells (4h stimulation, Cr51 assay).
Test code(s): SPL1
Enhanced NK cell cytotoxicity with Interleukin 2 stimulation against K562 cells (4h stimulation, Cr51 assay).
Test code(s): SPL2
MCW-CIRL
Flow cytometry assay using CFSE-labeled K562 target cells and 7-AAD incorporation by apoptotic target cells following culture with PBMC’s at various Effector:Target ratios. Test includes evaluation of CD107a surface expression/degranulation following exposure of NK cells to K562 cells.
Test code(s): CYTAPO
MONTREAL-SJH
Degranulation of NK cells by flow cytometry.
Test code(s): Dégranulation de NK
NSUH
NK function test measures CD107a surface expression after exposing NK cells to K562 cells.
Test code(s): NK function
QUEST
Natural Killer Cells, Functional. PBMC isolation from whole blood, incubation with fluorescently labeled K562 at various E:T target ratios, following incubation percent lysis is determined by flow cytometry.
Test code(s): 34184
UCLA-UIC
Flow cytometry assay using CFSE-labeled K562 target cells and Sytox Red incorporation by apoptotic target cells following culture with PBMCs at various Effector:Target ratios.
Test code(s): NKCC
VIRACOR
BMC isolation from whole blood, incubation with K562 (fluorescently labeled) at various E:T target ratios, following incubation percent lysis is determined by flow cytometry.
Test code(s): 30035
