DISEASE-SPECIFIC FLOW CYTOMETRY TESTS OR PANELS
ALPS Panel
ARUP
Autoimmune Lymphoproliferative Profile. Reported; % Alpha/Beta+, CD4-, CD8-, Absolute Alpha/Beta+, CD4-, CD8-, % CD5+, CD20+, Absolute CD5+, CD20+, % CD3+, HLA-DR+, Absolute CD3+, HLA-DR+. By Quantitative Flow Cytometry.
Test code(s): 0095505
BCH-CFCL
Immunophenotyping of γδ and CD4-CD8- αβ T cells, which reports: CD3+CD4-CD8-TCR alpha/beta T cells, CD3+TCR gamma/delta T cells.
Test code(s): ALPS Panel
CALGARY-CLS
The % and absolute count of CD3, CD3+4-8-TCRαβ+ DNT cells are reported as well as the % of DNT that express CD95, TCRαβ, HLA-DR and CD45R (B220). Additional antibodies to CD45R, CD25, HLA-DR CD19, and CD27 are used in an ALPS scoring system.
Test code(s): ALPS
CCHMC-DIL
ALPS panel uses antibodies against CD3, CD4, CD8, CD45, alpha beta TCR, CD25, CD27, HLA-DR, CD19, CD5, CD27, and B220 to create a panel that quantifies double negative T cells, CD27+ B cells, and CD25 and HLA-DR expression on T cells to rank the likelihood of ALPS.
Test code(s): ALPS Panel by Flow
CHCO- Immunology Lab
Autoimmune Lymphoproliferative Syndrome (ALPS). Reports percentage of TCRαβ+CD4-CD8- T cells
Test code(s): LAB8532
CHLA-DIFL
ALPS Panel Reports % CD3+CD4-CD8-αβ+, % CD3+CD4-CD8-γδ+, and %CD3+αβ+, %CD3+γδ+.
Test code(s): ALPS Panel Flow
MAYO-ML
Uses antibodies against CD3, CD4, CD8, CD45, alpha beta TCR, and B220 to quantify Double Negative T Cells, analyze B220 expression.
Test code(s): ALPS; #82449
MCW-CIRL
AILYMP Panel: CD3, CD4, CD8, CD16, CD56, CD19, CD14, CD45, CD45RA, CD45RO, HLA-DR, CD21, CD27, IgD, TCRαβ, TCRgd, B220.
ALPS Panel measures the percentage of the TCR alpha/beta positive CD4 and CD8 double negative T cells, referred to as TCR-DNTCs.
Test code(s): AILYMP
MONTREAL-SJH
CD3+CD4−CD8−/TCRαβ double negative T cells enumeration.
Test code(s): CD4(-)CD8(-)/ TCRαβ
NJH-ADL
Reports percentages of DNTs using a CD3/CD48/TCRαβ panel.
Test code(s): ALPS
NSUH
T-lymphocyte TCR subset uses CD3, CD4, CD8, TCRα/β, TCR γ/δ, B220, CD20, CD5, and CD45. Percentages are reported.
Test code(s): ALPS
BAFF-R and TACI Expression on B cells
MAYO-ML
Reports the percent of CD19+ B cells expressing TACI and BAFF-R.
Test code(s): CVID; #87993
NJH-ADL
Reports BAFF-R qualitatively as present/absent and includes commentary comparing the expression level to that of a date matched normal control.
Test code(s): BAFFR
BTK Protein Flow
ARUP
Bruton Tyrosine Kinase (BTK) Protein Expression by Flow Cytometry (Qualitative Flow Cytometry).
Test code(s): 2012002
CALGARY-CLS
Bruton Tyrosine Kinase (BTK) Protein Expression by Flow Cytometry in B cells and monocytes.
Test code(s): BTK
MAYO-ML
Analyzes BTK expression in B cells and monocytes.
Test code(s): BTK; #89011
MCW-CIRL
BTK Panel includes: CD3, CD4, CD8, CD19, BTK, CD16, CD56, CD14, CD45
Evaluates BTK expression in B cells and monocytes.
Test code(s): BTK
CD18, CD11a/CD11b, and CD15s Expression (Screening for LAD)
ARUP
Leukocyte Adhesion Deficiency Panel. Panel measures CD11b, CD15, and CD18 on neutrophils. By Semi-Quantitative Flow Cytometry.
Test code(s): 2004359
BCH-CFCL
Leukocyte Adhesion Deficiency, which reports: CD18+ cells, CD15s+ cells.
Test code(s): LAD Panel
CALGARY-CLS
Measures expression of CD18, CD11a, CD11b, CD11c (Type I LAD) and CD15s (Type II LAD) on the surface of granulocytes.
Test code(s): LAD
CCHMC-DIL
Measures CD18 and CD11b expression on neutrophils.
Test code(s): Neutrophil Adhesion Mrkrs: CD18/11b
CHCO- Immunology Lab
Leukocyte Adhesion Deficiency 1. Qualitative report (normal/abnormal) of CD18 and CD11b expression on neutrophils and monocytes
Test code(s): LAB9077
CHLA-DIFL
Leukocyte Adhesion Deficiency Type-1. Measures expression of CD18, CD11a, CD11b and CD15s on the surface of granulocytes.
Test code(s): Leukocyte Adhesion Deficiency Type 1
MAYO-ML
Measures CD18, CD11a, and CD11b on neutrophils. Provides % data for control and patient in interpretive report for abnormal results.
Test code(s): LAD1; #81155
MCW-CIRL
NPF Panel includes: CD45, CD15, CD18
CD18 and CD15 expression is evaluated on granulocytes.
Test code(s): NPF (formerly NEUOXB)
NJH-ADL
Qualitative analysis of CD11a,b,c and CD18 on neutrophils.
Test code(s): ADHM
NSUH
LAD panel uses CD11a, CD11b, CD11c, and CD18. Percentages are reported.
Test code(s): LAD
QUEST
Leukocyte Adhesion Deficiency (LAD) Evaluation (CD15s, CD18). Flow cytometry panel measures CD11b, CD15, and CD18 expression on neutrophils.
Test code(s): 19815
CD40 Expression
CALGARY-CLS
CD40 expression on B cells is assessed. Testing includes the CD40L (CD154) assay.
Test code(s): LINK
MAYO-ML
The cell surface expression of CD40 by B cells (CD19+) is determined and expressed as being present or absent.
Test code(s): CD40; #89009
MCW-CIRL
HIGM Panel Includes: CD3, CD4, CD8, CD154, CD40-Fc, CD19, CD40, CD16, CD56, CD14, CD45
Measures CD40 expression on B cells and CD40L expression on activated CD4 cells. Measures binding of CD40L on activated CD4 cells to recombinant CD40 (CD40-muIg).
Test code(s): HIGM
NJH-ADL
CD40 expression on CD19+ B cells. Qualitative interpretation – present/absent is reported.
Test code(s): CD40M
CD40L Expression
BCH-CFCL
Immunophenotyping of CD40 Ligand (CD154)- positive T lymphocytes, which reports: CD3+CD8-CD154+, CD3+CD8-CD69+.
Test code(s): CD40 Ligand assay
CALGARY-CLS
CD40L (CD154) expression is assessed on CD3+4+8- cells after PMA/Ionomycin activation. Testing includes assessment of CD40 on B cells.
Test code(s): LINK
CCHMC-DIL
Evaluation of CD40L Upregulation (and ICOS Upregulation). Also assesses CD40L binding to CD40-Ig Fusion Protein.
Test code(s): CD40L / ICOS
MAYO-ML
Evaluation of CD40L Upregulation, also includes assessment of binding to receptor using soluble CD40-muIg.
Test code(s): XHIM; #82964
MCW-CIRL
HIGM Panel Includes: CD3, CD4, CD8, CD154, CD40-Fc, CD19, CD40, CD16, CD56, CD14, CD45
Measures CD40 expression on B cells and CD40L expression on activated CD4 cells. Measures binding of CD40L on activated CD4 cells to recombinant CD40 (CD40-muIg).
Test code(s): HIGM
HIGM Panel Includes: CD3, CD4, CD8, CD154, CD40-Fc, CD19, CD40, CD16, CD56, CD14, CD45
Measures CD40 expression on B cells and CD40L expression on activated CD4 cells. Measures binding of CD40L on activated CD4 cells to recombinant CD40 (CD40-muIg).
Test code(s): HIGM
NJH-ADL
Analyzes CD40L expression in whole blood, gating on CD3+/CD8- T cells. Reports qualitative result – absent/present.
Test code(s): CD40L
DOCK8 Protein Flow
CALGARY-CLS
Assesses DOCK8 protein expression in peripheral blood lymphocyte subsets (T, B, NK).
Test code(s): DOCK8
CHCO- Immunology Lab
DOCK8 expression: Reports expression of DOCK8 in CD3+T cells, CD19+B cell and CD14+monocytes qualitatively. Mean fluorescence intensity of DOCK8 expression is reported
Test code(s): LAB935
MAYO-ML
The test detects the expression of dedicator of cytokinesis 8 (DOCK8) in T cells, B cells, NK cells, and monocytes in the peripheral blood.
It can be used as a screening step prior to genetic testing for DOCK8; to confirm the finding of an established pathogenic alteration in DOCK8 at the protein level; to examine a reported variant of undetermined significance (VUS); and to evaluate the potential presence of somatic reversion in a patient with DOCK8 deficiency.
It can help distinguish DOCK8 deficiency from conditions with overlapping clinical manifestations, including Job syndrome (AD-HIES), ZNF341 deficiency, and severe atopic dermatitis.
Test code(s): DOCK 8
MONTREAL-SJH
DOCK8 detection by flow cytometry.
Test code(s): Marqueurs intracytoplasmique: Autres and indicate yourself
Flow Cytometry Screening for SCID (Related to IL2RG and IL7RA)
CALGARY-CLS
Assesses both CD132 and CD127 on peripheral blood lymphocytes:
Test code(s): CD127/CD132
FOXP3 Expression
CALGARY-CLS
Reports the % and absolute count of Tregs determined by CD3+ CD4+ lowCD127+ and CD25+.
Test code(s): TREG
CCHMC-DIL
Quantifies the percent of T regs (CD25+ CD127lo) as a percentage of the CD4+ T cells, and quantifies the percentage of T regs, which express FOXP3.
Test code(s): FoxP3
CHCO- Immunology Lab
Regulatory T cells: Reports percentage of CD3+CD4+CD25+FOXP3+ T cells and mean fluorescence intensity of FoxP3
Test code(s): LAB9111
CHLA-DIFL
Regulatory T-Cell (T Reg). Percent of CD4+ CD25+ CD127dim is reported.
Test code(s):
MCW-CIRL
Treg panel includes: CD3, CD4, CD8, CD45RA, CD45RO, CD19, CD16, CD56, CD14, CD45, HLA-DR, CD25, FoxP3.
Evaluates lymphocyte subpopulations and the presence of Foxp3+ T regulatory cells.
Test code(s): TREG
Measures Foxp3+, CD127, CD25 expression on CD4 cells.
Test code(s): TREG
MONTREAL-SJH
Percentages of regulatory T cells: CD4+ CD25high CD127- Foxp3+ by flow cytometry.
Test code(s): FOXp3
NJH-ADL
National Jewish Health using a 4-color panel, CD3/4/CD25/ FoxP3, gating on CD25highFoxP3+ cells. Reports percentages.
Test code(s): FOXP3
NSUH
Regulatory T-Lymphocytes uses CD4, CD25, CD127, and FoxP3. Percentages are reported.
Test code(s): FoxP3
ICOS Expression
CALGARY-CLS
Reports ICOS (CD278) expression on CD3+4+8- cells pre and post stimulation with PMA/Ionomycin.
Test code(s): ICOS
CCHMC-DIL
The CD40L Assay includes measurement of ICOS before and after activation of T Cells.
Test code(s): CD40L / ICOS
NJH-ADL
Analyzes ICOS expression in whole blood, gating on CD3+/CD8- T cells. Reports qualitative result – absent/present.
Test code(s): ICOS
LRBA Expression
CALGARY-CLS
Analyzes lipopolysaccharide Responsive Beige-like Anchor (LRBA) protein expression in CD3+ T cells. Percentage and median fluorescent intensity compared to a simultaneously run normal control is reported.
Test code(s): LRBA
MAYO-ML
The test determines the percentage and intensity of expression of lipopolysaccharide-responsive beige-like anchor (LRBA) protein on T cells and B cells in the peripheral blood.
It can be used as a screening step prior to genetic testing for LRBA; to confirm the finding of an established pathogenic alteration in LRBA at the protein level; and to examine the effect of reported genetic variants of undetermined significance (VUS) on LRBA protein expression.
It can help distinguish LRBA deficiency from conditions with overlapping clinical manifestations, including immune dysregulation and autoimmunity, such as immune dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX)-like syndromes; early onset hypogammaglobulinemia; common variable immune deficiency (CVID); inflammatory bowel disease (IBD); and autoimmune lymphoproliferative syndrome (ALPS).
Test code(s): LRBA
MHC Class I and Class II Expression
CCHMC-DIL
Class I (HLA-A, B and C) and Class II (HLA-DR) epitope expression on peripheral blood lymphocytes and monocytes are performed by multiparameter flow cytometry.
Test code(s): MHC Class I & II
CHLA-DIFL
Bare lymphocyte syndrome - MHC Class II deficiency assay.
Test code(s): T Cell Extended
MCW-CIRL
CVID Panel:CD3, CD4, CD8, CD16, CD56, CD19, CD21, CD27, IgD, HLA-DR, CD45RA, CD45RO, CD14, CD45.
Reports percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD56+), (CD56+, CD3-), (CD56+, CD3+), and (CD56+, CD16+), and B lymphocytes (CD19+). T Cell subset percentages reported are activated T cells (HLA-DR+CD4+) and (HLA-DR+CD8+), T cell memory (CD4+CD45RA+) and (CD4+CD45RO+). A CD4:CD8 ratio is also reported. Reports percentage and absolute number of CD19+ as well as percentages of B cell subsets.
Test code(s): CVID
PPID2 Panel: CD3, CD4, CD8, CD45RA, CD45RO, CD16, CD56, CD19, CD14, CD45, HLA-DR.
Reports percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD56+), (CD56+, CD3-), (CD56+, CD3+), and (CD56+, CD16+), and B lymphocytes (CD19+). T Cell subset percentages reported are activated T cells (HLA-DR+CD4+) and (HLA-DR+CD8+), T cell memory (CD4+CD45RA+) and (CD4+CD45RO+). A CD4:CD8 ratio is also reported.
Test code(s): PID2
MONTREAL-SJH
Test of HLA class I expression.
Test code(s): HLA class I
Test of HLA class II expression on B lymphocytes (CD19+) and monocytes (CD14+).
Test code(s): HLA class II
Perforin Screening for Perforin Deficiency/HLH due to PRF1 Mutations
CALGARY-CLS
Assesses perforin and granzyme B expression in NK cells and CD8+ T cells.
Test code(s): HLH
CCHMC-DIL
Analyzes perforin expression in CD8+ T cells, NK cells. Also measures and reports granzyme B expression.
Test code(s): Perforin/Granzyme B
CHCO- Immunology Lab
Perforin expression: Reports mean fluorescence intensity of perforin expression in NK cells and CD8+ T cells
Test code(s): LAB9360
MCW-CIRL
Pergra Panel includes: CD3, CD4, CD8, CD56, CD16, CD19, CD14, CD45, Perforin, Granzyme B
Perforin and Granzyme B expression in CD56+/CD16+ and CD56+/CD16- cells.
Test code(s): PERGRA
MONTREAL-SJH
Perforin detection by flow cytometry.
Test code(s): Perforine
SAP Screening for SAP Deficiency/XLP1
CALGARY-CLS
Measures SAP expression in T and NK cells.
Test code(s): XLP1
CCHMC-DIL
Analyzes SAP expression in T cells and NK cells.
Test code(s): SAP (XLP1)
MCW-CIRL
XLP Panel Includes: CD3, CD4, CD8, CD45RA, CD45RO, CD19, CD16, CD56, CD14, CD45, HLA-DR, SAP, XIAP
Determines the presence of SAP and XIAP expression in lymphocytes to evaluate XLP type 1 and 2.
Test code(s): XLP
MONTREAL-SJH
SAP detection by flow cytometry.
Test code(s): Marqueurs intracytoplasmique: Autres and indicate yourself
WASP Screening for Wiskott-Aldrich Syndrome
CALGARY-CLS
Measures WAS in peripheral blood lymphocytes and monocytes and reports percentage and median fluorescent channel compared to a simultaneously run normal control.
Test code(s): WASP
CCHMC-DIL
Regular Panel (WASP) determines WASP expression in peripheral blood lymphocytes and reports expression as a ratio to control cells.
Test code(s): WASP
WAS Transplant Monitor Panel measures WASP expression in T cells, NK cells, B cells, monocytes, and granulocytes.
Test code(s): WASP Transplant Monitor
XIAP Screening for XIAP Deficiency/XLP2
CALGARY-CLS
Assay measures both SAP and XIAP expression in lymphocyte subsets:
Test code(s): XLP2
CCHMC-DIL
Assay measures XIAP expression in T cells, NK cells, B cells, Monocytes, and Granulocytes.
Test code(s): XIAP (XLP2)
MCW-CIRL
Panel Includes: CD3, CD4, CD8, CD45RA, CD45RO, CD19, CD16, CD56, CD14, CD45, HLA-DR, SAP, XIAP
Determines the presence of SAP and XIAP expression in lymphocytes to evaluate XLP type 1 and 2.
Test code(s): XLP
MONTREAL-SJH
XIAP detection by flow cytometry.
Test code(s): Marqueurs intracytoplasmique: Autres and indicate yourself
ZAP-70 screening for SCID due to ZAP-70 deficiency
CALGARY-CLS
ZAP-70 protein expression is measured in T and NK cell subsets.
Test code(s): ZAP70 SCID
CCHMC-DIL
Assay measures ZAP-70 expression in T cells and NK cells.
Test code(s): ZAP-70
