T CELL FUNCTION TESTS, INCLUDING RESPONSES TO MITOGENS/ANTIGENS, AND ASSESMENT OF CYTOTOXICITY
ARUP
Lymphocyte Mitogen Proliferation. 3H Thymidine incorporation assay measuring lymphocyte proliferation to the nonspecific mitogens phytohemagglutinin (PHA), concanavalin A (Con A), and pokeweed.
Test code(s): 0096043
Lymphocyte Antigen and Mitogen Proliferation Panel. 3H Thymidine incorporation assay measuring lymphocyte proliferation to phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen.
Test code(s): 0096056
Lymphocyte Antigen and Mitogen Proliferation Panel with Cytokine Response. Cell culture and multiplex bead assay, which measures lymphocyte proliferation and cytokine release following stimulation with Candida and tetanus antigens and the mitogens phytohemagglutinin, concanavalin A, and pokeweed mitogen.
Test code(s): 20131174
BCH-CFCL
T cell Mitogen-induced Lymphocyte Proliferation (Phytohemagglutinin, Concanavalin A, Anti-CD3), and T cell Antigen-induced Lymphocyte Proliferation (Candida Albicans, Tetanus Toxoid).
Test code(s): T cell Mitogen Proliferation and T cell Antigen Proliferation
CALGARY-CLS
Ability of mononuclear cells to proliferate in response to PWM, ConA, PHA and StaphA is assessed by flow cytometry cell counts and reported as a proliferation index.
Test code(s): MSA
CCHMC-DIL
3H Thymidine incorporation assay measuring lymphocyte proliferation to the antigens Candida albicans and Tetanus/Diphtheria toxoid.
Test code(s): Antigen Stimulation
3H Thymidine incorporation assay measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Concanvalin A (Con A), and Pokeweed (PWM).
Test code(s): Mitogen Stimulation
CD8+ T cell degranulation (CD107a) following exposure to anti-CD3 coated P815 cells.
Test code(s): T cell degran
CHCO- Immunology Lab
Lymphocyte and T cell proliferation to PHA. Flow cytometry based assay. Reports percentage of proliferating CD45+ lymphocytes and CD3+ T cells in response to 3 concentrations of PHA
Test code(s): LAB9101
CHLA-DIFL
3H Thymidine incorporation assay measuring lymphocyte proliferation to Phytohemagglutinin, Concanavalin A, pokeweed mitogens, and several antigens including CMV, HSV, VZV, ADV, Candida, CD3CD28 and tetanus antigens. Note: CD3CD28 induced proliferation by special request.
Test code(s): PWM Blastogenesis, ConA, PHA Blastogenesis, CMV Blastogenesis, HSV Blastogenesis, VZV Blastogenesis, Candida blastogenesis, ADV Blastogenesis, Tetanus Blastogenesis.
IOWA
Lymphocyte Proliferation to Mitogens. 3H Thymidine incorporation assay measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Concanvalin A (CON A), Pokeweed mitogen (PWM), Interleukin 2 (IL2) and anti-CD3.
Test code(s): SPL3
Lymphocyte Proliferation to Antigens. 3H Thymidine incorporation assay measuring lymphocyte proliferation to the antigens Tetanus/Diphtheria, Candida albicans and alloantigen.
Test code(s): SPL4
MAYO-ML
CMV-specific CD8 T cell immune competence is evaluated using MHC class I tetramers (flow assay also includes a quantitative component for CMV-specific CD8 T cells for 5 HLA alleles – A1, A2, B7, B8 and B35). Functional component includes CD107a/b expression and IFN-g production of CMV-specific CD8 T cells.
Test code(s): CMVC8; #88826
CD8 T cell degranulation (CD107a) and measurement of IFN-g after CD8 T cell activation with PMA/ionomycin.
Test code(s): GLIC; #89317
Flow based methodology measuring lymphocyte proliferation to an anti-CD3 panel (soluble anti-CD3, aCD3+aCD28, aCD3+ IL-2).
Test code(s): LPA3P; #62205
Flow based methodology measuring lymphocyte proliferation to the antigens Candida albicans and tetanus toxoid.
Test code(s): LPAG; #60592
Flow based methodology measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA) and Pokeweed (PWM).
Test code(s): LPMG; #60591
MCW-CIRL
CFSE-labeled T cells are assessed for their ability to proliferate to cytokines: anti-CD3, anti-CD3/IL-2, anti-CD3/IL-7, anti-CD3/IL-15, anti-CD3/anti-CD28.
Test code(s): TINTL
Flow-based methodology. CFSE-labeled T cells are assessed for their ability to proliferate to mitogens: ConA/IL-2, PHA/IL-2, and anti-CD3 with and without IL-2. Standard screening test for T cell function.
Test code(s): TMITO
MONTREAL-SJH
Degranulation CD3+CD8+ blasts (cytotoxic CD8 T lymphocytes) by flow cytometry.
Test code(s): Dégranulation des lymphocytes T
T cell proliferation after PHA and anti-CD3 (OKT3) antibody stimulation by flow cytometry.
Test code(s): PHA, OKT3
NJH-ADL
3H Thymidine incorporation in response to tetanus toxoid (3 concentrations) and Candida.
Test code(s): LCAND, LTET
3H Thymidine incorporation assay to measure lymphocyte proliferation to Phytohemagglutinin (PHA), Concanvalin A (Con A), and Pokeweed (PWM) (3 concentrations each). Available separately with
Test code(s): LPHA, LCONA, LPMW, or together:
NSUH
Lymphocyte proliferation to Antigen uses 3H thymidine incorporation method to measure lymphocyte proliferation to Tetanus toxoid and Candida albicans.
Test code(s): Antigen
Lymphocyte proliferation to Mitogen uses 3H thymidine incorporation method to measure lymphocyte proliferation to PHA, ConA, and PWM.
Test code(s): Mitogen
QUEST
Candida-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Candida.
Test code(s): 17270
Tetanus-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Tetanus Toxoid.
Test code(s): 17274
Tuberculin PPD-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Tuberculin PPD (TB PPD).
Test code(s): 17680
Mitogen-induced Lymphocyte Proliferation Panel. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Phytohemagglutinin (PHA), Concanavalin A (Con A), Pokeweed Mitogen (PWM). Test available by prior arrangement only.
Test code(s): 91976
Antigen-induced Lymphocyte Proliferation Panel (Candida, Tetanus, TB PPD). Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Candida, Tetanus Toxoid, Tuberculin PPD (TB PPD). Test available by prior arrangement only.
Test code(s): 91977
Mitogen- and Antigen-induced Lymphocyte Proliferation Panel. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Phytohemagglutinin (PHA), Concanavalin A (Con A), Pokeweed Mitogen (PWM), Candida, Tetanus Toxoid, Tuberculin PPD (TB PPD). Test available by prior arrangement only.
Test code(s): 91978
Phytohemagglutinin (PHA)-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Phytohemagglutinin (PHA). Test available by prior arrangement only.
Test code(s): 91980
Pokeweed Mitogen (PWM)-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Pokeweed Mitogen (PWM). Test available by prior arrangement only.
Test code(s): 91981
Concanavalin A (Con A)-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Concanavalin A (Con A). Test available by prior arrangement only.
Test code(s): 91982
UCLA-UIC
Flow-based methodology measuring lymphocyte proliferation to the mitogen Phytohemagglutinin (PHA) and to the antigens Candida Albicans and Tetanus Toxoid.
Test code(s): LPA-1M2A
Flow-based methodology measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Pokeweed (PWM) and Concanavalin A (Con A).
Test code(s): LPA-3M
Flow-based methodology measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Pokeweed (PWM) and Concanavalin A (Con A), and to the antigens Candida Albicans and Tetanus Toxoid.
Test code(s): LPA-3M2A
VIRACOR
T cell Function Analysis: PBMC isolation from whole blood, stimulation with various antigens or mitogens, flow cytometry analysis for CD69 upregulation and interferon gamma production.
Test code(s): 403066 and 403065
Intracellular Cytokine Panel: Whole blood is stimulated with PMA and ionomycin. Following stimulation cells are stained with antibodies to identify the cell population (CD4, CD8, NK and NKT cells) and cytokine (interferon gamma, IL4, or TNF) of interest.
Test code(s): 404474
