GENERAL IMMUNOPHENOTYPING (FLOW CYTOMETRY)
B Cell Enumeration and/or Extended Phenotyping
Comprehensive B cell phenotyping: Reports percentages of the following CD19+ B cells: naïve (CD27-IgD+), total non-class switched memory (CD27+IgD+), class-switched memory (CD27+IgD-), CD21low, transitional (IgM+CD38+), plasmablasts (IgM-CD38++), IgM only CD27+ B cells, IgM+IgD+ CD27+ B cells, IgM-IgD- class-switched CD27+ B cells
Test code(s): LAB9066
Total B Cell (CD19, CD20) Quantitation – This test is meant primarily for monitoring patients receiving B cell-depleting therapies where an entire lymphocyte subset quantitation is not required. This assay is available in regular and high-sensitivity format, and the latter will be determined by the Laboratory Director, based on clinical context and/or communication with ordering physician. This panel includes both CD19 and CD20 markers enabling its use in patients receiving specific B cell-depleting therapies without provoking analytical interference in the assay based on half-life of the drug.
Test code(s): TBCQN
B Cell Phenotyping
B cell Number. Reports percentage and absolute number of B cells.
Memory B cells. Reports percentages of B cells that are switched memory (CD27+ and IgD-), unswitched memory (CD27+ and IgD+), and naive (CD27- IgD+).
CVID Panel. Reports CD19+ CD21low CD38low B cells, Transitional B cells (CD24hi CD38high), Plasmablasts (CD24low CD38high), and Marginal zone like B cells (CD24high CD38 low).
Test code(s):
Immunodeficiency screen panel includes percentages and absolute numbers of T cells, B cells, NK cells, TNK (CD3+16/56+), CD3+4+, CD3+8+, DNT (CD3+4-8-), CD4/CD8 ratio, Naïve B cells (CD19+IgD+CD27-), Memory non-class switched B cells (CD19+IgD+CD27+), Memory class switched B cells (CD19+IgD-CD27+), Naïve Helper T cells (CD3+4+CD45RA+CD27+), Terminally Differentiated Helper T cell (CD3+4+45RA+27-), Central Memory Helper T cell (CD3+4+45RA-27+), Effector Memory Helper T cell (CD3+4+45RA-27-), Naïve Cytotoxic T cells (CD3+8+CD45RA+CD27+), Terminally Differentiated Cytotoxic T cell cell (CD3+8+45RA+27-), Central Memory Cytotoxic T cell (CD3+8+45RA-27+), Effector Memory Cytotoxic T cell (CD3+8+45RA-27-). CD45RA/CD45RO also assessed.
Test code(s): Test code(s): IDEF
B Cell Panel: Reports several Immature, Transitional, Naïve, Memory, and Class-Switched Memory B cell populations, and also Plasma-Blast populations.
Test code(s):
CVID Panel:CD3, CD4, CD8, CD16, CD56, CD19, CD21, CD27, IgD, HLA-DR, CD45RA, CD45RO, CD14, CD45.
Reports percentage and absolute number of CD19+. Reports percentages of B cell subsets that are switched memory (CD27+, IgD-), unswitched memory (CD27+, IgD+), and naive (CD27-, IgD+). Additional B Cell memory markers include (CD21+, CD27-), (CD21+, CD27+) and (CD21-, CD27+). Panel also includes percentages and absolute numbers of T and NK cells.
Test code(s): CVID
Lymphocyte subset phenotyping by quantitative flow cytometry: B lymphocyte subsets CD19+, CD19+CD27+, CD19+CD20+.
Test code(s): Test code(s): CD19, CD27/CD19, CD20
Both the T-cell memory and the B-cell panels include a basic panel for lymphocyte enumeration, which reports the percentages and absolute numbers of T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), CD4:CD8 ratio, natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+), followed by an advanced panel to identify (i) T-memory: naïve (CD45RA+/CD45RO-) and Memory (CD45RA-/CD45RO+) CD4+ and CD8+ T cells; (ii) B-cell: Memory (CD27+), IgM Memory (CD27+IgM+IgD-), Unswitched Memory (CD27+IgM+IgD+), Switched Memory (CD19+CD27+IgD-IgM-) and Immature (CD21low) CD19+ B cells.
Test code(s):
CD4+ Recent Thymic Emigrants
CD4+ T-Cell Recent Thymic Emigrants (RTEs). Reports percentage and absolute numbers of CD4+CD31+CD45RA+ (RTEs). By Quantitative Flow Cytometry.
Test code(s): 2010179
Immunophenotyping of Recent Thymic Emigrants, which reports: CD4+CD31+CD45RA+Recent Thymic Emigrants.
Test code(s): RTE Panel
Reports the % and absolute count of CD3+CD4+CD45RA+CD31+ recent thymic emigrants.
Test code(s): RTE
CD45RA/RO panel includes evaluation of CD31 expression as a marker of recent thymic emigrants.
Test code(s): CD45RA/RO
Naïve, memory and recent thymic emigrant T cell panel: Reports percentage of naïve (CD45RA+), memory (CD45RO+), recent thymic emigrants (CD4+CD31+), effector memory (CD45RA-CCR7-), central memory (CD45RA-CCR7+), TEMRA (CD45RA+CCR7-) CD4 and CD8 T cells, and CD4+CD31+ recent thymic emigrants
Test code(s): LAB849
The percentages and absolute numbers of lymphocytes that are newly formed CD4+ cells with following phenotypes of CD4+CD45+RA+ cells: CD3+4+/RA+RO+, CD3+4+/RA+/CD31+ and CD3+4+/RA+62L+ are reported.
Test code(s): T Cell Subsets (Memory and Naïve)
Reports the percentage of CD4+ 45RA+ CD31+ T cells.
Test code(s): CD4RT; #89504
Naïve/memory T lymphocyte subsets: CD4+CD45RO+, CD4+CD45RA+, CD4+CD45RA+ CD31+, and CD8+ CD45RA+ CCR7+ (naïve), CD8+CD45RA-CCR7+ (CM), CD8+CD45RA-CCR7- (TEM), CD8+CD45RA+CCR7- (TEMRA).
Test code(s): CD45RA/CD4, CD45RA-31/CD4, CD45RO/CD4, CD45RA/CD8, CD45RA-31/CD8, CD45RO/CD8
Identifies RTEs using a 4-color panel: CD3/CD4/CD45RA/CD31. Reports percentages.
Test code(s): CD4RTE
Lymphocyte Subset Enumeration
Natural Killer Cells Enumeration: The results reported are the percentage of lymphocytes that are Natural killer (NK) cells and the absolute count of NK-cells per µL of blood. Only those cells that express CD45 and either CD16 or CD56 (or both) but not CD3 or CD19 are reported. By Quantitative Flow Cytometry.
Test code(s): 0092404
Lymphocyte Subset Panel 3: T-Cell Subsets (CD4 and CD8), Absolute Counts Only. By Quantitative Flow Cytometry. Reported; Abs CD3, CD4, CD8, and abs CD4:CD8 ratio.
Test code(s): 0095853
Lymphocyte Subset Panel 1: CD4 Absolute Count Only. Reported; abs CD4. By Quantitative Flow Cytometry.
Test code(s): 0095854
Lymphocyte Subset Panel 2: CD4 Percent and Absolute. Reported; % CD4, and Abs CD4. By Quantitative Flow Cytometry.
Test code(s): 0095885
Lymphocyte Subset Panel 5: Total Lymphocyte Enumeration. Reported; % CD3, abs CD3, % CD4, abs CD4, % CD8, abs CD8, CD4: CD8 ratio, % CD19, abs CD19, % NK-cells, abs NK-cells. By Quantitative Flow Cytometry.
Test code(s): 0095892
Isolated Panel for T cells (Monitoring post ATG): Lymphocyte Transplantation CD3. Reported: WBC (K/µL), % Lymph, Absolute Lymph Count, %CD3, abs CD3. By Quantitative Flow Cytometry.
Test code(s): 0095949
Lymphocyte Subset Panel 4: T-Cell Subsets Percent and Absolute. Reported; %CD3, abs CD3, %CD4, abs CD4, %CD8, abs CD8, CD4:CD8 Ratio. By Quantitative Flow Cytometry.
Test code(s): 0095950
Analysis of lymphocytes populations, which reports: The percentages and absolute numbers of lymphocytes that are CD3+ (Total T cells), CD3+CD4+ (T helper cells), CD3+CD8+ (T cytotoxic cells), CD4:CD8 ratio, CD3-CD19+ (B cells), CD-CD16/56+ (NK cells).
Test code(s): Lymphocyte Subsets or T/B/NK Panel
Immunodeficiency screen panel includes percentages and absolute numbers of T cells, B cells, NK cells, TNK (CD3+16/56+), CD3+4+, CD3+8+, DNT (CD3+4-8-), CD4/CD8 ratio, Naïve B cells (CD19+IgD+CD27-), Memory non-class switched B cells (CD19+IgD+CD27+), Memory class switched B cells (CD19+IgD-CD27+), Naïve Helper T cells (CD3+4+CD45RA+CD27+), Terminally Differentiated Helper T cell (CD3+4+45RA+27-), Central Memory Helper T cell (CD3+4+45RA-27+), Effector Memory Helper T cell (CD3+4+45RA-27-), Naïve Cytotoxic T cells (CD3+8+CD45RA+CD27+), Terminally Differentiated Cytotoxic T cell cell (CD3+8+45RA+27-), Central Memory Cytotoxic T cell (CD3+8+45RA-27+), Effector Memory Cytotoxic T cell (CD3+8+45RA-27-). CD45RA/CD45RO also assessed.
Test code(s): IDEF
The percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+) are reported.
Test code(s): Lymphocyte Subsets
TBNK: Reports percentages and absolute counts of CD3+, CD4+, CD8+, CD19+ and CD16/56+ lymphocytes (CBC required).
Test code(s): LAB7755
T Cell Subsets: Reports percentage and absolute counts of CD3+, CD4+ and CD8+ T cells (CBC required)
Test code(s): LAB7756
CD3: Reports percentage and absolute counts of CD3+ T cells (CBC required)
Test code(s): LAB7758
T cell Extended panel: The percentages and absolute numbers of lymphocytes that are CD3+ (Total T cells), CD3+CD4+ (T helper cells), CD3+CD8+ (T cytotoxic cells), CD4:CD8 ratio, CD3-CD19+ (B cells), CD-CD16/56+ (Natural Killer (NK) cells), and CD3+ HLA-DR+ (Activated T cells) are reported.
Test code(s): T-Cell Extended
T cell Standard panel: The percentages and absolute numbers of lymphocytes that are CD3+ (Total T cells), CD3+CD4+ (T helper cells), CD3+CD8+ (T cytotoxic cells), CD4:CD8 ratio, and CD3+ HLA-DR+ (Activated T cells) are reported.
Test code(s): T-Cell Standard
The percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+) are reported.
Test code(s): TBBS; #9336
AT4 Panel: CD3, CD4, CD8, CD14, CD45
To monitor patient's individual CD4 and CD8 T cell populations. Panel is routinely used in determining therapeutic considerations for HIV+ patients. Reports percentages and absolute numbers of lymphocytes that are T cells (CD3+ Total T Cells), T-helper (CD3+, CD4+) and T-cytotoxic (CD3+, CD8+).
Test code(s): AT4
PID1 Panel: CD3, CD4, CD8, CD16, CD56, CD19, CD14, CD45.
Reports percentages and absolute numbers of lymphocytes that are T cells (CD3+ Total T Cells), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD56+), (CD56+, CD3-), (CD56+, CD3+), and (CD56+, CD16+), and B lymphocytes (CD19+). A CD4:CD8 ratio is also reported.
Test code(s): PID1
PPID2 Panel: CD3, CD4, CD8, CD45RA, CD45RO, CD16, CD56, CD19, CD14, CD45, HLA-DR.
Reports percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD56+), (CD56+, CD3-), (CD56+, CD3+), and (CD56+, CD16+), and B lymphocytes (CD19+). T Cell subset percentages reported are activated T cells (HLA-DR+CD4+) and (HLA-DR+CD8+), T cell memory (CD4+CD45RA+) and (CD4+CD45RO+). A CD4:CD8 ratio is also reported.
Test code(s): PID2
Lymphocyte subset phenotyping by quantitative flow cytometry: B lymphocyte subsets CD19+, CD19+CD27+, CD19+CD20+.
Test code(s): CD19, CD27/CD19, CD20
Lymphocyte subset phenotyping by quantitative flow cytometry: NK cells: CD3-CD56+.
Test code(s): CD3 (-) CD56 (+)
Lymphocyte subset phenotyping by quantitative flow cytometry: T lymphocytes: CD3+, CD3+CD4+, CD3+CD8.
Test code(s): CD3, CD4, CD8
Lymphocyte Subset (T, B, NK Cells) Quantitation in Blood – This is a flow cytometric lymphocyte subset quantitation (CD45+ ALC, CD14 for monocyte exclusion, CD3+, CD4+ and CD8+ T cells, CD19/20+ B cells, CD16/56+ NK cells, CD4:CD8 ratio, CD3+CD4-CD8- DNT cells. This test is useful for the evaluation of patients with primary immunodeficiencies (inborn errors of immunity), patients undergoing solid-organ transplantation (during induction and post-transplant monitoring), patients undergoing hematopoietic cell transplantation (HCT) for assessment of immune reconstitution and competence, patients receiving immunomodulatory therapies, and any clinical context where a more detailed lymphocyte subset evaluation is needed (outside a CBC + differential). This test can be available as STAT (TBNKTX) for patients in whom results are urgently needed, and in a non-rush format (TBNK). Results are typically reported within 24-48h from receipt of sample in the laboratory.
Test code(s): TBNK
Lymphocyte Subset (T, B, NK Cells) Quantitation in Bronchoalveolar Lavage – This is a flow cytometric lymphocyte subset quantitation (CD45+ ALC, CD14 for monocyte exclusion, CD3+, CD4+ and CD8+ T cells, CD19/20+ B cells, CD16/56+ NK cells, CD4:CD8 ratio, CD3+CD4-CD8- DNT cells. This test is useful for the evaluation of patients with primary immunodeficiencies (inborn errors of immunity), patients undergoing solid-organ transplantation (during induction and post-transplant monitoring), patients undergoing hematopoietic cell transplantation (HCT) for assessment of immune reconstitution and competence, patients receiving immunomodulatory therapies, and any clinical context where a more detailed lymphocyte subset evaluation is needed (outside a CBC + differential). Results are typically reported within 24-48h from receipt of sample in the laboratory.
Test code(s): TBNKBL
CD3/4/8: The percentages and absolute numbers are reported.
Test code(s): CD3SB
CD4/8: The percentages and absolute numbers are reported.
Test code(s): CD48
Individual markers - CD4: The percentage and absolute number are reported.
Test code(s): CD4M
Individual markers - CD16/56: The percentage and absolute number are reported.
Test code(s): NKMK
Lymphocyte Phenotyping Panel: CD3+, CD3+/CD4+, CD3+/CD8+, CD16/56 and CD19+. CD4/CD8 ratio reported. The percentages and absolute numbers are reported.
Test code(s): TBCDC
CD3/4/8/19: The percentages and absolute numbers are reported.
Test code(s): TBSBS
NSUH
Lymphocyte subset uses CD3, CD4, CD8, CD19, CD16, and CD56. Report includes percentages and absolute counts. CD4 to CD8 ratio is also reported.
Test code(s): TnB
Both the T-cell memory and the B-cell panels include a basic panel for lymphocyte enumeration, which reports the percentages and absolute numbers of T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), CD4:CD8 ratio, natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+), followed by an advanced panel to identify (i) T-memory: naïve (CD45RA+/CD45RO-) and Memory (CD45RA-/CD45RO+) CD4+ and CD8+ T cells; (ii) B-cell: Memory (CD27+), IgM Memory (CD27+IgM+IgD-), Unswitched Memory (CD27+IgM+IgD+), Switched Memory (CD19+CD27+IgD-IgM-) and Immature (CD21low) CD19+ B cells.
Test code(s): T-MEM or B-CELL
Lymphocyte/T cell Activation
Panel 1: CD3+4+ T cells are assessed for surface expression of CD154, CD69, HLA-DR, CD143, CD25, CD71, and CD96 pre and post PMA/Ionomycin stimulation. Panel 2: CD8+ T cells are assessed for expression of CD45RO and HLA-DR primarily for use in investigation of Omenn’s Syndrome or HLH. Antigen density of CD5 on CD3+8+ cells is also reported for HLH investigations.
Test code(s): LAM
Measures CD4+ T cell surface expression of CD69, CD25, CD154, CD134, CD95, CD71, and HLA-DR.
Test code(s): Lymphocyte Activation Markers
Lymphocyte and T cell proliferation to PHA. Flow cytometry based assay. Reports percentage of proliferating CD45+ lymphocytes and CD3+ T cells in response to 3 concentrations of PHA
Test code(s): LAB9101
T Cell Activation Panel. The following populations are reported in percentages: CD25+CD3+/CD3, DR+CD3+/CD3, CD69+CD3+/CD3, CD134+CD3+/CD3, CD25+CD4+/CD3, DR+CD4+/CD3, CD69+CD4+/CD3, CD134+CD4+/CD3.
Test code(s): T Cell Subsets Activated T
The T cell phenotyping assay includes evaluation of activated (CD4+25+ and MHC class II-positive) CD4+ and CD8+ T cells.
Test code(s): TCP; #89319
T cell activation panel: Includes CD4, CD8, CD69, and CD25 at 4 hours and 4 days after activation to measure early and late activation events:
Test code(s): TCACT
Sainte Justine Hospital: Percentages of activated T cells for HLA class II DR expression on CD3+, CD3+CD4+, and CD3+CD8+ by flow cytometry.
Test code(s): HLA class II/CD3, HLA class II/CD4, HLA class II/CD8
Monocyte and Dendritic Cell Phenotyping
Dendritic Cell and Monocyte Enumeration. This test enumerates plasmacytoid dendritic cells (pDC), myeloid dendritic cells (mDC), and classical monocytes.
It can be used as part of the diagnostic assessment of patients suspected of defects in innate immunity, particularly those in monocyte and dendritic cell development, which can manifest in isolation or as part of a broader clinical phenotype.
Test code(s): DCME
Monocyte Subset Quantitation – Monocytes reflect the monocyte/macrophage or mononuclear phagocytes in circulation. The majority of CD14high cells found in human blood are classified as classical monocytes while a very minor population (in healthy controls) of cells express low CD14 and high CD16 and are described as non-classical monocytes. The population between these two are referred to as the transitional subset. It is useful to quantitate and evaluate monocyte subsets in the context of certain primary immunodeficiencies (inborn errors of immunity) where such cell populations are clinically informative. Monocyte subsets can also be skewed in the context of severe infection, HIV-1 infection, therapy with steroids and other immunomodulatory agents, and are critical regulators of inflammation. There is also some evidence that the absolute count of transitional monocytes can predict cardiovascular events. Monocyte subsets in blood varies substantially during life from the neonatal period into adulthood.
Test code(s): MOSQN
NK Cell Phenotyping
Natural Killer Cells Enumeration. The results reported are the percentage of lymphocytes that are Natural killer (NK) cells and the absolute count of NK-cells per µL of blood. Only those cells that express CD45 and either CD16 or CD56 (or both) but not CD3 or CD19 are reported. By Quantitative Flow Cytometry.
Test code(s): 0092404
Natural Killer Cell and Natural Killer T-Cell Panel. The panel reports frequencies and absolute cell counts of NK and NKT-cells, also including T and non-T cells: CD3-CD16-/+CD56bright/dim (total NK-cells), CD3-CD16+CD56dim (cytotoxic NK-cells), CD3-CD16-CD56bright (cytokine secreting NK-cells), CD3-CD57+ (CD57+NK-cells), CD3+CD56+ (CD56+ NKT-cells), CD3+CD57+ (CD57+ NKT-cells), CD45+CD3+ (T-cells) and CD45+CD3- (Non T-cells).
Test code(s): 2013805
Immunophenotyping of Natural Killer cells which reports: percentages of CD56 high NK cells, CD56+CD16+ NK cells, and CD56-CD16 + NK cells.
Test code(s): NK Cell Phenotype
Pergra Panel includes: CD3, CD4, CD8, CD56, CD16, CD19, CD14, CD45, Perforin, Granzyme B
Perforin and Granzyme B expression in CD56+/CD16+ and CD56+/CD16- cells.
Test code(s): PERGRA
NK Cell Subset Quantitation – This test is for quantitation of the 2 major NK cell subsets in blood - cytotoxic NK cells and cytokine-producing NK cells. Useful for evaluation of patients with primary immunodeficiencies (inborn errors of immunity), in the context of hematopoietic cell transplantation and any clinical context where the distribution of these two NK cell subsets in blood is required. This test does not assess for NK cell receptors.
Test code(s): NKSQN
NKT cell Subsets (including invariant NKT cells)
The percentage and absolute count of CD3+ TCRvalpha24+ TCRvbeta11+ iNKT cells is reported.
Test code(s): INKT
Quantification of TCRValpa24 and TCR Vbeta 11 expression on invariant NKT cells.
Test code(s): iNKT
Quantification of MAIT (Va 24 Vb 11) and iNKT (Va 24 Vb 11) cells.
Test code(s): NKT
Regulatory T Cell Analysis and/or FOXP3 Expression
Regulatory T-Cell Panel. Reported: Percent and absolute numbers of CD4+ CD25+ CD127Low (Tregs), and CD45RA and CD45RO also included in the panel. By Quantitative Flow Cytometry.
Test code(s): 2010172
Immunophenotyping of Regulatory T cells, which reports: CD4+CD25+CD127+ T cells, CD4+CD25highCD127low T cells.
Test code(s): Treg Panel
Reports the % and absolute count of Tregs determined by CD3+ CD4+ lowCD127+ and CD25+.
Test code(s): TREG
Quantifies the percent of T regs (CD25+ CD127 lo) as a percentage of the CD4+ T cells, and quantifies the percentage of T regs, which express FOXP3.
Test code(s): FoxP3
Regulatory T cells: Reports percentage of CD3+CD4+CD25+FOXP3+ T cells and mean fluorescence intensity of FoxP3
Test code(s): LAB9111
Regulatory T-Cell (T Reg). Percent of CD4+ CD25+ CD127dim is reported.
Test code(s):
Identifies percentages and absolute numbers of CD4+CD25+CD127loCD45RO+ (Nat Tregs) and CD4+CD25+CD127loCD45RA+ Naive Tregs.
Test code(s): TREGS; #89318
Percentages of regulatory T cells: CD4+ CD25high CD127- Foxp3+ by flow cytometry.
Test code(s): FOXp3
National Jewish Health using a 4-color panel, CD3/4/CD25/ FoxP3, gating on CD25highFoxP3+ cells. Reports percentages.
Test code(s): FOXP3
NSUH
Regulatory T-Lymphocytes uses CD4, CD25, CD127, and FoxP3. Percentages are reported.
Test code(s): FoxP3
T Cell Phenotyping (Naïve T Cells, Memory T Cells, Recent Thymic Emigrants/Extended Phenotyping)
Lymphocyte Subset Panel 6: Total Lymphocyte Enumeration with CD45RA and CD45RO. Reported; %CD3, abs CD3, % CD4, abs CD4, % CD45RA, abs CD45RA, % CD45RO, abs CD45RO, % CD8, abs CD8, CD4:CD8 Ratio, % CD19, abs CD19, % NK-cells, abs NK-cells. By Quantitative Flow Cytometry.
Test code(s): 0095862
Lymphocyte Subset Panel 7: Congenital Immunodeficiencies. Reported; %CD2, abs CD2, % CD3, Abs CD3, %HLA-DR, abs HLA-DR, % CD4, abs CD4, % CD45RA, Abs CD45RA, % CD8, abs CD8, CD4: CD8 ratio, % CD19, abs CD19, % NK-cells, abs NK-cells. By Quantitative Flow Cytometry.
Test code(s): 0095899
Immunophenotyping of Naïve and Memory T cells, which reports: TEMRA CD4+CD45RA+CCR7-T cells, Naïve CD4+CD45RA+CCR7+T cells, Effector memory CD4+CD45RA-CCR7-T cells, Central memory CD4+CD45RA-CCR7+T cells, TEMRA CD8+CD45RA+CCR7-T cells, Naïve CD8+CD45RA+CCR7+T cells, Effector memory CD8+CD45RA-CCR7-T cells, Central memory CD8+CD45RA-CCR7+T cells.
Test code(s): T Cell Memory Panel
Immunodeficiency screen panel includes percentages and absolute numbers of T cells, B cells, NK cells, TNK (CD3+16/56+), CD3+4+, CD3+8+, DNT (CD3+4-8-), CD4/CD8 ratio, Naïve B cells (CD19+IgD+CD27-), Memory non-class switched B cells (CD19+IgD+CD27+), Memory class switched B cells (CD19+IgD-CD27+), Naïve Helper T cells (CD3+4+CD45RA+CD27+), Terminally Differentiated Helper T cell (CD3+4+45RA+27-), Central Memory Helper T cell (CD3+4+45RA-27+), Effector Memory Helper T cell (CD3+4+45RA-27-), Naïve Cytotoxic T cells (CD3+8+CD45RA+CD27+), Terminally Differentiated Cytotoxic T cell (CD3+8+45RA+27-), Central Memory Cytotoxic T cell (CD3+8+45RA-27+), Effector Memory Cytotoxic T cell (CD3+8+45RA-27-). CD45RA/CD45RO also assessed.
Test code(s): IDEF
CD45RA/RO Assay. Reports percentages of naïve, central memory, effector memtory, and TEMRA CD4+ and CD8+ populations using a combination of markers including CD45RA, CD45RO, CCR7, and CD27. Also reports CD31 expression as a marker of recent thymic emigrants.
Test code(s):
Naïve, memory and recent thymic emigrant T cell panel: Reports percentage of naïve (CD45RA+), memory (CD45RO+), recent thymic emigrants (CD4+CD31+), effector memory (CD45RA-CCR7-), central memory (CD45RA-CCR7+), TEMRA (CD45RA+CCR7-) CD4 and CD8 T cells, and CD4+CD31+ recent thymic emigrants
Test code(s): LAB849
T follicular helper cells (Tfh): Reports percentage of CD3+CD4+CXCR5+ T cells
Test code(s): LAB9410
Naïve T Cell Subsets. The percentages and absolute numbers of lymphocytes that are newly formed CD4+ cells with following phenotypes of CD4+CD45+RA+ cells: CD3+4+/RA+RO+, CD3+4+/RA+/CD31+ and CD3+4+/RA+62L+ are reported.
Test code(s): T cell Subsets (Memory and Naïve)
Quantitates naïve (global and CD62L+), memory (global, central, and effector memory), and activated (CD4+25+ and MHC class II-positive) CD4+ and CD8+ T cells using antibodies directed against CD3, CD4, CD8, CD45RO, CD45RA, HLADR, CD27, CD62L, CD25, and CD28.
Test code(s): TCP; #89319
CVID Panel:CD3, CD4, CD8, CD16, CD56, CD19, CD21, CD27, IgD, HLA-DR, CD45RA, CD45RO, CD14, CD45.
Reports percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD56+), (CD56+, CD3-), (CD56+, CD3+), and (CD56+, CD16+), and B lymphocytes (CD19+). T Cell subset percentages reported are activated T cells (HLA-DR+CD4+) and (HLA-DR+CD8+), T cell memory (CD4+CD45RA+) and (CD4+CD45RO+). A CD4:CD8 ratio is also reported. Reports percentage and absolute number of CD19+ as well as percentages of B cell subsets.
Test code(s): CVID
PID2 Panel: CD3, CD4, CD8, CD45RA, CD45RO, CD16, CD56, CD19, CD14, CD45, HLA-DR.
Reports percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD56+), (CD56+, CD3-), (CD56+, CD3+), and (CD56+, CD16+), and B lymphocytes (CD19+). T Cell subset percentages reported are activated T cells (HLA-DR+CD4+) and (HLA-DR+CD8+), T cell memory (CD4+CD45RA+) and (CD4+CD45RO+). A CD4:CD8 ratio is also reported
Test code(s): PID2
Naïve/memory T lymphocytes: CD4+CD45RO+, CD4+CD45RA+, CD4+CD45RA+CD31+/ CD8+CD45RA+CCR7+ (naïve), CD8+CD45RA-CCR7+ (CM), CD8+CD45RA-CCR7- (TEM), CD8+CD45RA+CCR7- (TEMRA).
Test code(s): CD45RA/CD4, CD45RA-31/CD4, CD45RO/CD4, CD45RA/CD8, CD45RA-31/CD8, CD45RO/CD8
National Jewish Health runs a memory and naïve T cell panel that includes CD3/4/CD45RA/CD45RO.
Test code(s): C4RARO
NSUH
Naïve/memory T-Lymphocyte subset uses CD3, CD4, CD8, CD45RA, and CD45RO. Percentages are reported.
Test code(s): CD45RARO
The T-cell memory panel includes a basic panel for lymphocyte enumeration, which reports the percentages and absolute numbers of T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), CD4:CD8 ratio, natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+), followed by an advanced panel to identify naïve (CD45RA+/CD45RO-) and Memory (CD45RA-/CD45RO+) CD4+ and CD8+ T cells.
Test code(s): T-MEM
TCR V Beta Repertoire
T-Cell Clonality by Flow Cytometry Analysis of TCR V-beta. Quantitates 24 alpha-beta T cell antigen receptors.
Test code(s): 0093199
Provides % and graphic representation of 24 vbeta repertoire antigens on CD3, CD3+4+ and CD3+8+ subsets.
Test code(s): TCR FLOW
Quantitates 24 antigens of the TCR V β repertoire of T lymphocytes.
Test code(s): TCR V Beta Repertoire
TCR V Beta Repertoire. Quantitates 24 V Beta TCR family receptors in total CD3, CD4, and CD8 T-cell subsets.
Test code(s):
See Molecular Tests: TCR Vb Spectratyping
Test code(s):
Percentages of TCR Vb repertoire (flow cytometry) in CD4+ and CD8+ T cells. Test Code: V Beta 2/CD4, V Beta 2/CD8, 6 V Beta /CD4, 6 V Beta /CD4
Test code(s):
See Molecular Tests: TCR Vb Spectratyping
Test code(s):
TCR α/β - TCR γ/δ
Immunophenotyping of γδ and CD4-CD8- αβ T cells, which reports: CD3+CD4-CD8-TCR alpha/beta T cells, CD3+TCR gamma/delta T cells. See also ALPS panel.
Test code(s): ALPS Panel
Reports the % and absolute count of TCRα/β and TCRγ/δ positive T cells:
Test code(s): TCRABGD
Quantifies the percentages of T cells expression α/β TCR and γ/δ TCR.
Test code(s): TCR α/β TCR γ/δ
TCRαβ and TCRγδ subsets: Reports percentage of TCRαβ and TCRγδ T cells
Test code(s): LAB7755
Quantifies the percentages of T cells expression α/β TCR and γ/δ TCR.
Test code(s): TCR α/β TCR γ/δ
Percentages of TCR α/β /CD3 and TCR γ/δ /CD3.
Test code(s): TCR α/β TCR γ/δ /CD3
Quantifies the percentages of TCRα/β and γ/δ T cells.
Test code(s): TCABGD
NSUH
T-lymphocyte TCR subset uses CD3, CD4, CD8, TCRα/β, and TCRγ/δ. Percentages are reported.
Test code(s): TCR
DISEASE-SPECIFIC FLOW CYTOMETRY TESTS OR PANELS
ALPS Panel
Autoimmune Lymphoproliferative Profile. Reported; % Alpha/Beta+, CD4-, CD8-, Absolute Alpha/Beta+, CD4-, CD8-, % CD5+, CD20+, Absolute CD5+, CD20+, % CD3+, HLA-DR+, Absolute CD3+, HLA-DR+. By Quantitative Flow Cytometry.
Test code(s): 0095505
Immunophenotyping of γδ and CD4-CD8- αβ T cells, which reports: CD3+CD4-CD8-TCR alpha/beta T cells, CD3+TCR gamma/delta T cells.
Test code(s): ALPS Panel
The % and absolute count of CD3, CD3+4-8-TCRαβ+ DNT cells are reported as well as the % of DNT that express CD95, TCRαβ, HLA-DR and CD45R (B220). Additional antibodies to CD45R, CD25, HLA-DR CD19, and CD27 are used in an ALPS scoring system.
Test code(s): ALPS
ALPS panel uses antibodies against CD3, CD4, CD8, CD45, alpha beta TCR, CD25, CD27, HLA-DR, CD19, CD5, CD27, and B220 to create a panel that quantifies double negative T cells, CD27+ B cells, and CD25 and HLA-DR expression on T cells to rank the likelihood of ALPS.
Test code(s): ALPS Panel by Flow
Autoimmune Lymphoproliferative Syndrome (ALPS). Reports percentage of TCRαβ+CD4-CD8- T cells
Test code(s): LAB8532
ALPS Panel Reports % CD3+CD4-CD8-αβ+, % CD3+CD4-CD8-γδ+, and %CD3+αβ+, %CD3+γδ+.
Test code(s): ALPS Panel Flow
Uses antibodies against CD3, CD4, CD8, CD45, alpha beta TCR, and B220 to quantify Double Negative T Cells, analyze B220 expression.
Test code(s): ALPS; #82449
AILYMP Panel: CD3, CD4, CD8, CD16, CD56, CD19, CD14, CD45, CD45RA, CD45RO, HLA-DR, CD21, CD27, IgD, TCRαβ, TCRgd, B220.
ALPS Panel measures the percentage of the TCR alpha/beta positive CD4 and CD8 double negative T cells, referred to as TCR-DNTCs.
Test code(s): AILYMP
CD3+CD4−CD8−/TCRαβ double negative T cells enumeration.
Test code(s): CD4(-)CD8(-)/ TCRαβ
Reports percentages of DNTs using a CD3/CD48/TCRαβ panel.
Test code(s): ALPS
NSUH
T-lymphocyte TCR subset uses CD3, CD4, CD8, TCRα/β, TCR γ/δ, B220, CD20, CD5, and CD45. Percentages are reported.
Test code(s): ALPS
BAFF-R and TACI Expression on B cells
Reports the percent of CD19+ B cells expressing TACI and BAFF-R.
Test code(s): CVID; #87993
Reports BAFF-R qualitatively as present/absent and includes commentary comparing the expression level to that of a date matched normal control.
Test code(s): BAFFR
BTK Protein Flow
Bruton Tyrosine Kinase (BTK) Protein Expression by Flow Cytometry (Qualitative Flow Cytometry).
Test code(s): 2012002
Bruton Tyrosine Kinase (BTK) Protein Expression by Flow Cytometry in B cells and monocytes.
Test code(s): BTK
Analyzes BTK expression in B cells and monocytes.
Test code(s): BTK; #89011
BTK Panel includes: CD3, CD4, CD8, CD19, BTK, CD16, CD56, CD14, CD45
Evaluates BTK expression in B cells and monocytes.
Test code(s): BTK
CD18, CD11a/CD11b, and CD15s Expression (Screening for LAD)
Leukocyte Adhesion Deficiency Panel. Panel measures CD11b, CD15, and CD18 on neutrophils. By Semi-Quantitative Flow Cytometry.
Test code(s): 2004359
Leukocyte Adhesion Deficiency, which reports: CD18+ cells, CD15s+ cells.
Test code(s): LAD Panel
Measures expression of CD18, CD11a, CD11b, CD11c (Type I LAD) and CD15s (Type II LAD) on the surface of granulocytes.
Test code(s): LAD
Measures CD18 and CD11b expression on neutrophils.
Test code(s): Neutrophil Adhesion Mrkrs: CD18/11b
Leukocyte Adhesion Deficiency 1. Qualitative report (normal/abnormal) of CD18 and CD11b expression on neutrophils and monocytes
Test code(s): LAB9077
Leukocyte Adhesion Deficiency Type-1. Measures expression of CD18, CD11a, CD11b and CD15s on the surface of granulocytes.
Test code(s): Leukocyte Adhesion Deficiency Type 1
Measures CD18, CD11a, and CD11b on neutrophils. Provides % data for control and patient in interpretive report for abnormal results.
Test code(s): LAD1; #81155
NPF Panel includes: CD45, CD15, CD18
CD18 and CD15 expression is evaluated on granulocytes.
Test code(s): NPF (formerly NEUOXB)
Qualitative analysis of CD11a,b,c and CD18 on neutrophils.
Test code(s): ADHM
NSUH
LAD panel uses CD11a, CD11b, CD11c, and CD18. Percentages are reported.
Test code(s): LAD
Leukocyte Adhesion Deficiency (LAD) Evaluation (CD15s, CD18). Flow cytometry panel measures CD11b, CD15, and CD18 expression on neutrophils.
Test code(s): 19815
CD40 Expression
CD40 expression on B cells is assessed. Testing includes the CD40L (CD154) assay.
Test code(s): LINK
The cell surface expression of CD40 by B cells (CD19+) is determined and expressed as being present or absent.
Test code(s): CD40; #89009
HIGM Panel Includes: CD3, CD4, CD8, CD154, CD40-Fc, CD19, CD40, CD16, CD56, CD14, CD45
Measures CD40 expression on B cells and CD40L expression on activated CD4 cells. Measures binding of CD40L on activated CD4 cells to recombinant CD40 (CD40-muIg).
Test code(s): HIGM
CD40 expression on CD19+ B cells. Qualitative interpretation – present/absent is reported.
Test code(s): CD40M
CD40L Expression
Immunophenotyping of CD40 Ligand (CD154)- positive T lymphocytes, which reports: CD3+CD8-CD154+, CD3+CD8-CD69+.
Test code(s): CD40 Ligand assay
CD40L (CD154) expression is assessed on CD3+4+8- cells after PMA/Ionomycin activation. Testing includes assessment of CD40 on B cells.
Test code(s): LINK
Evaluation of CD40L Upregulation (and ICOS Upregulation). Also assesses CD40L binding to CD40-Ig Fusion Protein.
Test code(s): CD40L / ICOS
Evaluation of CD40L Upregulation, also includes assessment of binding to receptor using soluble CD40-muIg.
Test code(s): XHIM; #82964
HIGM Panel Includes: CD3, CD4, CD8, CD154, CD40-Fc, CD19, CD40, CD16, CD56, CD14, CD45
Measures CD40 expression on B cells and CD40L expression on activated CD4 cells. Measures binding of CD40L on activated CD4 cells to recombinant CD40 (CD40-muIg).
Test code(s): HIGM
HIGM Panel Includes: CD3, CD4, CD8, CD154, CD40-Fc, CD19, CD40, CD16, CD56, CD14, CD45
Measures CD40 expression on B cells and CD40L expression on activated CD4 cells. Measures binding of CD40L on activated CD4 cells to recombinant CD40 (CD40-muIg).
Test code(s): HIGM
Analyzes CD40L expression in whole blood, gating on CD3+/CD8- T cells. Reports qualitative result – absent/present.
Test code(s): CD40L
DOCK8 Protein Flow
Assesses DOCK8 protein expression in peripheral blood lymphocyte subsets (T, B, NK).
Test code(s): DOCK8
DOCK8 expression: Reports expression of DOCK8 in CD3+T cells, CD19+B cell and CD14+monocytes qualitatively. Mean fluorescence intensity of DOCK8 expression is reported
Test code(s): LAB935
The test detects the expression of dedicator of cytokinesis 8 (DOCK8) in T cells, B cells, NK cells, and monocytes in the peripheral blood.
It can be used as a screening step prior to genetic testing for DOCK8; to confirm the finding of an established pathogenic alteration in DOCK8 at the protein level; to examine a reported variant of undetermined significance (VUS); and to evaluate the potential presence of somatic reversion in a patient with DOCK8 deficiency.
It can help distinguish DOCK8 deficiency from conditions with overlapping clinical manifestations, including Job syndrome (AD-HIES), ZNF341 deficiency, and severe atopic dermatitis.
Test code(s): DOCK 8
DOCK8 detection by flow cytometry.
Test code(s): Marqueurs intracytoplasmique: Autres and indicate yourself
Flow Cytometry Screening for SCID (Related to IL2RG and IL7RA)
Assesses both CD132 and CD127 on peripheral blood lymphocytes:
Test code(s): CD127/CD132
FOXP3 Expression
Reports the % and absolute count of Tregs determined by CD3+ CD4+ lowCD127+ and CD25+.
Test code(s): TREG
Quantifies the percent of T regs (CD25+ CD127lo) as a percentage of the CD4+ T cells, and quantifies the percentage of T regs, which express FOXP3.
Test code(s): FoxP3
Regulatory T cells: Reports percentage of CD3+CD4+CD25+FOXP3+ T cells and mean fluorescence intensity of FoxP3
Test code(s): LAB9111
Regulatory T-Cell (T Reg). Percent of CD4+ CD25+ CD127dim is reported.
Test code(s):
Treg panel includes: CD3, CD4, CD8, CD45RA, CD45RO, CD19, CD16, CD56, CD14, CD45, HLA-DR, CD25, FoxP3.
Evaluates lymphocyte subpopulations and the presence of Foxp3+ T regulatory cells.
Test code(s): TREG
Measures Foxp3+, CD127, CD25 expression on CD4 cells.
Test code(s): TREG
Percentages of regulatory T cells: CD4+ CD25high CD127- Foxp3+ by flow cytometry.
Test code(s): FOXp3
National Jewish Health using a 4-color panel, CD3/4/CD25/ FoxP3, gating on CD25highFoxP3+ cells. Reports percentages.
Test code(s): FOXP3
NSUH
Regulatory T-Lymphocytes uses CD4, CD25, CD127, and FoxP3. Percentages are reported.
Test code(s): FoxP3
ICOS Expression
Reports ICOS (CD278) expression on CD3+4+8- cells pre and post stimulation with PMA/Ionomycin.
Test code(s): ICOS
The CD40L Assay includes measurement of ICOS before and after activation of T Cells.
Test code(s): CD40L / ICOS
Analyzes ICOS expression in whole blood, gating on CD3+/CD8- T cells. Reports qualitative result – absent/present.
Test code(s): ICOS
LRBA Expression
Analyzes lipopolysaccharide Responsive Beige-like Anchor (LRBA) protein expression in CD3+ T cells. Percentage and median fluorescent intensity compared to a simultaneously run normal control is reported.
Test code(s): LRBA
The test determines the percentage and intensity of expression of lipopolysaccharide-responsive beige-like anchor (LRBA) protein on T cells and B cells in the peripheral blood.
It can be used as a screening step prior to genetic testing for LRBA; to confirm the finding of an established pathogenic alteration in LRBA at the protein level; and to examine the effect of reported genetic variants of undetermined significance (VUS) on LRBA protein expression.
It can help distinguish LRBA deficiency from conditions with overlapping clinical manifestations, including immune dysregulation and autoimmunity, such as immune dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX)-like syndromes; early onset hypogammaglobulinemia; common variable immune deficiency (CVID); inflammatory bowel disease (IBD); and autoimmune lymphoproliferative syndrome (ALPS).
Test code(s): LRBA
MHC Class I and Class II Expression
Class I (HLA-A, B and C) and Class II (HLA-DR) epitope expression on peripheral blood lymphocytes and monocytes are performed by multiparameter flow cytometry.
Test code(s): MHC Class I & II
Bare lymphocyte syndrome - MHC Class II deficiency assay.
Test code(s): T Cell Extended
CVID Panel:CD3, CD4, CD8, CD16, CD56, CD19, CD21, CD27, IgD, HLA-DR, CD45RA, CD45RO, CD14, CD45.
Reports percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD56+), (CD56+, CD3-), (CD56+, CD3+), and (CD56+, CD16+), and B lymphocytes (CD19+). T Cell subset percentages reported are activated T cells (HLA-DR+CD4+) and (HLA-DR+CD8+), T cell memory (CD4+CD45RA+) and (CD4+CD45RO+). A CD4:CD8 ratio is also reported. Reports percentage and absolute number of CD19+ as well as percentages of B cell subsets.
Test code(s): CVID
PPID2 Panel: CD3, CD4, CD8, CD45RA, CD45RO, CD16, CD56, CD19, CD14, CD45, HLA-DR.
Reports percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD56+), (CD56+, CD3-), (CD56+, CD3+), and (CD56+, CD16+), and B lymphocytes (CD19+). T Cell subset percentages reported are activated T cells (HLA-DR+CD4+) and (HLA-DR+CD8+), T cell memory (CD4+CD45RA+) and (CD4+CD45RO+). A CD4:CD8 ratio is also reported.
Test code(s): PID2
Test of HLA class I expression.
Test code(s): HLA class I
Test of HLA class II expression on B lymphocytes (CD19+) and monocytes (CD14+).
Test code(s): HLA class II
Perforin Screening for Perforin Deficiency/HLH due to PRF1 Mutations
Assesses perforin and granzyme B expression in NK cells and CD8+ T cells.
Test code(s): HLH
Analyzes perforin expression in CD8+ T cells, NK cells. Also measures and reports granzyme B expression.
Test code(s): Perforin/Granzyme B
Perforin expression: Reports mean fluorescence intensity of perforin expression in NK cells and CD8+ T cells
Test code(s): LAB9360
Pergra Panel includes: CD3, CD4, CD8, CD56, CD16, CD19, CD14, CD45, Perforin, Granzyme B
Perforin and Granzyme B expression in CD56+/CD16+ and CD56+/CD16- cells.
Test code(s): PERGRA
Perforin detection by flow cytometry.
Test code(s): Perforine
SAP Screening for SAP Deficiency/XLP1
Measures SAP expression in T and NK cells.
Test code(s): XLP1
Analyzes SAP expression in T cells and NK cells.
Test code(s): SAP (XLP1)
XLP Panel Includes: CD3, CD4, CD8, CD45RA, CD45RO, CD19, CD16, CD56, CD14, CD45, HLA-DR, SAP, XIAP
Determines the presence of SAP and XIAP expression in lymphocytes to evaluate XLP type 1 and 2.
Test code(s): XLP
SAP detection by flow cytometry.
Test code(s): Marqueurs intracytoplasmique: Autres and indicate yourself
WASP Screening for Wiskott-Aldrich Syndrome
Measures WAS in peripheral blood lymphocytes and monocytes and reports percentage and median fluorescent channel compared to a simultaneously run normal control.
Test code(s): WASP
Regular Panel (WASP) determines WASP expression in peripheral blood lymphocytes and reports expression as a ratio to control cells.
Test code(s): WASP
WAS Transplant Monitor Panel measures WASP expression in T cells, NK cells, B cells, monocytes, and granulocytes.
Test code(s): WASP Transplant Monitor
XIAP Screening for XIAP Deficiency/XLP2
Assay measures both SAP and XIAP expression in lymphocyte subsets:
Test code(s): XLP2
Assay measures XIAP expression in T cells, NK cells, B cells, Monocytes, and Granulocytes.
Test code(s): XIAP (XLP2)
Panel Includes: CD3, CD4, CD8, CD45RA, CD45RO, CD19, CD16, CD56, CD14, CD45, HLA-DR, SAP, XIAP
Determines the presence of SAP and XIAP expression in lymphocytes to evaluate XLP type 1 and 2.
Test code(s): XLP
XIAP detection by flow cytometry.
Test code(s): Marqueurs intracytoplasmique: Autres and indicate yourself
ZAP-70 screening for SCID due to ZAP-70 deficiency
ZAP-70 protein expression is measured in T and NK cell subsets.
Test code(s): ZAP70 SCID
Assay measures ZAP-70 expression in T cells and NK cells.
Test code(s): ZAP-70
HIV (LIMITED FLOW CYTOMETRIC T CELL QUANTITATION)
Lymphocyte Subset Panel 3 - T-Cell Subsets (CD4 and CD8), Absolute Counts Only. Reported; Abs CD3, CD4, CD8, and abs CD4:CD8 ratio. By Quantitative Flow Cytometry.
Test code(s): 0095853
Lymphocyte Subset Panel 1 - CD4 Absolute Count Only. Reported; abs CD4. By Quantitative Flow Cytometry.
Test code(s): 0095854
Lymphocyte Subset Panel 2 - CD4 Percent and Absolute. Reported; % CD4, and Abs CD4. By Quantitative Flow Cytometry.
Test code(s): 0095885
Lymphocyte Subset Panel 4 - T-Cell Subsets Percent and Absolute, Whole Blood, Reported; %CD3, abs CD3, %CD4, abs CD4, %CD8, abs CD8, CD4:CD8 Ratio. By Quantitative Flow Cytometry.
Test code(s): 0095950
T Cell Standard. The percentages and absolute numbers of lymphocytes that are CD3 (Total T cells), CD3+CD4+ (T helper cells), CD3+CD8+ (T cytotoxic cells), CD4:CD8 ratio, CD 3+ HLA-DR+ (Activated T cells) are reported.
Test code(s): T cell Standard
The percentages and absolute counts for CD3+T cells, CD4+ T cells, CD8+ T cells and CD45+ absolute lymphocyte count.
Test code(s): TCD4 (#84348)
Absolute T4 Panel includes: CD3, CD4, CD8, CD14, CD45
To monitor patient's individual CD4 and CD8 T cell populations. Panel is routinely used in determining therapeutic considerations for HIV+ patients. Reports percentages and absolute numbers of lymphocytes that are T cells (CD3+ Total T Cells), T-helper (CD3+, CD4+) and T-cytotoxic (CD3+, CD8+).
Test code(s): AT4
CD3/4/8.
Test code(s): CD3SB
CD4/8.
Test code(s): CD48
Lymphocyte Subset Panel 4. Absolute Lymphocytes, Percentage CD4, Absolute CD4, Percentage CD8, Absolute CD8, CD4/CD8 Ratio (calculated).
Test code(s): 3061N
Lymphocyte Subset Panel 3. Absolute Lymphocytes, %CD 3 (Mature T-Cells), Absolute CD3+ Cells, %CD4 (Helper Cells), Absolute CD4+ Cells, %CD8 (Suppressor T-Cells), Absolute CD8+ Cells, Helper/Suppressor Ratio.
Test code(s): 5033N
Lymphocyte Subset Panel 5. Absolute Lymphocytes, Percentage CD4, Absolute CD4.
Test code(s): 5058N
DISEASE-SPECIFIC OR PHENOTYPE SPECIFIC FUNCTIONAL TESTS (EXCLUDING T CELL AND NK CELL FUNCTION TESTS)
CD40L Expression/Upregulation (Screening for XHIGM)
Immunophenotyping of CD40 Ligand (CD154)- positive T lymphocytes, which reports: CD3+CD8-CD154+, CD3+CD8-CD69+.
Test code(s): CD40 Ligand assay
CD40L (CD154) expression is assessed on CD3+4+8- cells after PMA/Ionomycin activation. Testing includes assessment of CD40 on B cells.
Test code(s): LINK
Evaluation of CD40L Upregulation, also includes assessment of binding to receptor using soluble CD40-muIg.
Test code(s): XHIM; #82964
HIGM Panel Includes: CD3, CD4, CD8, CD154, CD40-Fc, CD19, CD40, CD16, CD56, CD14, CD45
Measures CD40 expression on B cells and CD40L expression on activated CD4 cells. Measures binding of CD40L on activated CD4 cells to recombinant CD40 (CD40-muIg).
Test code(s): HIGM
Analyzes CD40L expression in whole blood, gating on CD3+/CD8- T cells. Reports qualitative result – absent/present.
Test code(s): CD40L
DHR/Oxidative Burst (Screening for CGD)
Neutrophil Oxidative Burst Assay (DHR). White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by Semi-Quantitative Flow Cytometry.
Test code(s): 0096657
Neutrophil’s Oxidative Burst, which reports: Neutrophil Oxidative Index (MFI DHR+ / MFI DHR-), and % Responding Polymorphonuclear Cells.
Test code(s): DHR assay
Functional CGD screening assay, using DHR123 loaded neutrophils pre and post stimulation with PMA to induce oxidative burst reaction.
Test code(s): NFUN
Classic dihydrorhodamine (DHR) 123 assay.
Test code(s): Neutrophil Oxidative Burst (DHR)
Dihydrorhodamine 123 Neutrophil oxidative burst (DHR). Reports stimulation index
Test code(s): LAB7757
Chronic granulomatous disease diagnostic screening assay for oxidative burst using dihydrorhodamine (DHR) 123.
Test code(s): CGD Flow
Classic dihydrorhodamine (DHR) 123 assay with PMA stimulation.
Test code(s): SPL8
Classic dihydrorhodamine (DHR) 123 assay.
Test code(s): DHR (for both PMA and fMLP stimulants); #62764; DHRP (only for PMA); #62765; DHRF (only for fMLP); #62766.
NPF Panel Includes: CD45, CD15, CD18
Dihydrorhodamine (DHR) 123 assay. Measures the ability of neutrophils to generate an oxidative burst following stimulation with PMA or fMLP. The expression of CD15 and CD18 on neutrophils is also measured. Screening test for chronic granulomatous disease (CGD) and leucocyte adhesion deficiency type I and type II.
Test code(s): NPF (Formerly NEUOXB)
NPF Panel: CD45, CD15, CD18
Dihydrorhodamine (DHR) 123 assay. Measures the ability of neutrophils to generate an oxidative burst following stimulation with PMA or fMLP. The expression of CD15 and CD18 on neutrophils is also measured. Screening test for chronic granulomatous disease (CGD) and leucocyte adhesion deficiency type I and type II.
Test code(s): NPT (formerly NEUOXB)
Neutrophil’s oxidation (DHR) by flow cytometry.
Test code(s): Activité Oxidative des Neutrophiles
Classic dihydrorhodamine (DHR) 123 assay. Offers NBT assay as back up.
Test code(s): DHR; NBT
NSUH
Neutrophil oxidation assay uses dihydrorhodamine (DHR) 123 and PMA. Report includes MFI ratio.
Test code(s): DHR
Neutrophil Function, Oxidative Burst. Classic dihydrorhodamine (DHR) 123 assay.
Test code(s): 37523
Classic dihydrorhodamine (DHR) 123 assay with PMA stimulation.
Test code(s): GOB
Oxidative Burst: Whole blood is stimulated with PMA or buffer alone, Formation of the reactive oxidants during the oxidative burst is monitored by the addition and enzymatic oxidation of a fluorogenic substrate, DHR 123. The level of reactive oxygen radicals is determined by flow cytometry.
Test code(s): 403002
DNA Double-strand Break Repair Assessment in Lymphocytes
DNA Double-strand Break Repair Assessment in Lymphocytes – This test is useful for the diagnosis of monogenic defects associated with defects in DNA repair and associated radiosensitivity (XCIND - X-ray irradiation susceptibility, Cancer predisposition, Immunodeficiency and Neurological Defects), bone marrow failure syndromes, short telomere syndromes, excessive toxicity to chemotherapy and radiation. This test can be used to develop appropriate conditioning regimen selection in patients with these defects who require hematopoietic cell transplantation. DDRFL primarily assesses for the presence of DNA double-strand breaks in each lymphocyte subset (T, B and NK cells) and the associated repair mechanism (NHEJ, non-homologous end-joining), and excessive cell death following irradiation of lymphocytes. Markers used in this assay include CD45, CD3, CD19, CD335 (NKp46), pATM, pSMC1 and gamma H2AX. The test measures viability in the sample at baseline (Day 0), 1h and 24h radiosensitivity, and 24h assessment of cell death and apoptosis after irradiation.
Test code(s): DDRFL
Functional Screen for XIAP Deficiency/XLP2
Assay tests TLR4 function by assessing the ability of monocytes to produce TNF-α in response to LPS and MDP (to test XIAP function) stimulation. Defective response can be seen in IRAK4/MyD88 deficiency and XIAP deficiency.
Test code(s): TLREC/XIAP
GMCSF Receptor Function
Measurement of STAT5 phosphorylation following GMCSF stimulation. (GMCSF autoantibody testing also available.)
Test code(s): GM-CSF Receptor Stimulation
Measures phosphorylation/dephosphorylation STAT5 (IL2, IL3, IL15, and GMCSF-induced) by flow cytometry.
Test code(s): Étude functionnelle and indicate yourself
ELISA measurement of GM-CSF autoantibodies, and reflex testing of P-STAT5 inhibition for positive samples.
Test code(s): GMCSFA
IL-10R Function Screen
Assay measures the ability of IL-10 to suppress IL-6 synthesis from monocytes in response to LPS stimulation, which is dependent on the functional integrity of the IL10 receptors and STAT3. Detects functional defects due to IL10R deficiency or autosomal dominant mutations in STAT3 leading to the Hyper-IgE Syndrome.
Test code(s): CYTIBD
Lymphocyte Apoptosis
Fas-mediated apoptosis assay. T cells are treated with APO-1-3 and protein A to induce cell death, following a 11 day culture, using Concanavalin A and IL-2. The % cell loss is calculated and reported.
Test code(s): Apoptosis (Fas, mediated)
Mendelian Susceptibility to Mycobacterial Disease
MSMD Panel: CD3, CD4, CD8, CD45RA, CD45RO, CD16, CD56, CD19, CD14, CD45, HLA-DR, CD212, CD119, IL-12, pSTAT1, pSTAT4
Measures expression of INF-R1 and IL12-1 receptors (CD119 and CD212), INF induced STAT1-phosphorylation and IL-12-induced STAT4-phosphorylation as well as IL12 production. Screening assay to detect genetic defects involving CD119, CD212, STAT1 and IL12 leading to an increased susceptibility to mycobacterial disease.
Test code(s): MSMD
Two tests can be ordered: 1 - IL-12/gIFN cytokines secretion (ELISA). 2 - Measures of phosphorylation/dephosphorylation of STAT1 in response to gIFN by flow cytometry.
Test code(s): Étude functionnelle and indicate yourself
Phenotypic analysis of IFNγR1 and IL-12 (beta chain) receptors.
Test code(s): IFNGR, IL12R
SCID Screening (IL2RG and JAK3)
Functional assay in which peripheral blood T cells are stimulated with IL-6 to induce phosphorylation of STAT3 or alternatively, stimulation with IL-2 to induce phosphorylation of STAT5.
Test code(s): PSTAT3 or PSTAT5
Analyzes phosphorylation of STAT5 in peripheral blood lymphocytes following stimulation of peripheral blood mononuclear cells with IL-2.
Test code(s): pSTAT5
Measures phosphorylation/dephosphorylation of STAT3 (IL10 and IL6-induced) and STAT5 (IL2, IL3, IL15, and GMCSF-induced) by flow cytometry.
Test code(s): Étude functionnelle and indicate yourself
TH17 Assay
Measures % of Th17 cells in the CD3+4+ subset following stimulation with PMA/Ionomycin.
Test code(s): TH17
Quantifies CD4+ CCR6+ T cells in comparison to an age-based range, and measures IL-17 production following stimulation with Phorbol 12-Myristate 13 Acetate (PMA) and Ionomycin which allows quantitation of TH17 cells.
Test code(s): Th-17 Enumeration
THIL17 Panel Includes: CD3, CD4, CD8, CD19, CD16, CD56, CD14, CD45, IFN-γ, IL-17
Assay measures T cell-specific IFN-γ and IL-17 production by PMA/Ionomycin stimulated PBMCs. Deficient expression is associated with hyper IgE syndrome (HIES)/Job’s Syndrome and also severe eczema.
Test code(s): THIL17
Measures IL-17 production following stimulation with Phorbol 12-Myristate 13 Acetate (PMA) and Ionomycin in CD4 T cells.
Test code(s): Étude functionnelle and indicate yourself
Offers % of TH1 (IFNg) and TH17 (IL-17) CD4 T cells in PBMC following PMA/Ionomycin stimulation.
Test code(s): TH17C
Toll-like Receptor Function (and Functional Screen for NEMO Deficiency)
Toll-Like Receptor Function. Results for TNF alpha, IL-1 beta, IL-6, and CXCL10 are reported as pg/mL. Method: Cell Culture/Quantitative Multiplex Bead Assay.
Test code(s): 0051589
Assay tests TLR4 function by assessing the ability of monocytes to produce TNF-α in response to LPS and MDP (to test XIAP function) stimulation.
Test code(s): TLREC
Assay tests TLR4 function by assessing the ability of monocytes to produce TNF-α in response to LPS and MDP (to test XIAP function) stimulation. Defective response can be seen in IRAK4/MyD88 deficiency and XIAP deficiency.
Test code(s): TLREC/XIAP
TLR 2-4 activation: CD62L shedding assay (only local samples for technical reasons) and IL-6 and TNF secretion (ELISA).
Test code(s): TLR2-4
TLR 3 activation in NK cells (CD69 and CD25 expression) by flow cytometry.
Test code(s): TLR3
Whole blood stimulation with multiple ligands, Lipoteichoic Acid (TLR2), Zymosan (TLR2/6), LPS (TLR4), Flagellin (TLR5), and Bacterial DNA (TLR9) followed by TNF-alpha analysis.
Test code(s): 403091
T CELL FUNCTION TESTS, INCLUDING RESPONSES TO MITOGENS/ANTIGENS, AND ASSESMENT OF CYTOTOXICITY
Lymphocyte Mitogen Proliferation. 3H Thymidine incorporation assay measuring lymphocyte proliferation to the nonspecific mitogens phytohemagglutinin (PHA), concanavalin A (Con A), and pokeweed.
Test code(s): 0096043
Lymphocyte Antigen and Mitogen Proliferation Panel. 3H Thymidine incorporation assay measuring lymphocyte proliferation to phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen.
Test code(s): 0096056
Lymphocyte Antigen and Mitogen Proliferation Panel with Cytokine Response. Cell culture and multiplex bead assay, which measures lymphocyte proliferation and cytokine release following stimulation with Candida and tetanus antigens and the mitogens phytohemagglutinin, concanavalin A, and pokeweed mitogen.
Test code(s): 20131174
T cell Mitogen-induced Lymphocyte Proliferation (Phytohemagglutinin, Concanavalin A, Anti-CD3), and T cell Antigen-induced Lymphocyte Proliferation (Candida Albicans, Tetanus Toxoid).
Test code(s): T cell Mitogen Proliferation and T cell Antigen Proliferation
Ability of mononuclear cells to proliferate in response to PWM, ConA, PHA and StaphA is assessed by flow cytometry cell counts and reported as a proliferation index.
Test code(s): MSA
3H Thymidine incorporation assay measuring lymphocyte proliferation to the antigens Candida albicans and Tetanus/Diphtheria toxoid.
Test code(s): Antigen Stimulation
3H Thymidine incorporation assay measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Concanvalin A (Con A), and Pokeweed (PWM).
Test code(s): Mitogen Stimulation
CD8+ T cell degranulation (CD107a) following exposure to anti-CD3 coated P815 cells.
Test code(s): T cell degran
Lymphocyte and T cell proliferation to PHA. Flow cytometry based assay. Reports percentage of proliferating CD45+ lymphocytes and CD3+ T cells in response to 3 concentrations of PHA
Test code(s): LAB9101
3H Thymidine incorporation assay measuring lymphocyte proliferation to Phytohemagglutinin, Concanavalin A, pokeweed mitogens, and several antigens including CMV, HSV, VZV, ADV, Candida, CD3CD28 and tetanus antigens. Note: CD3CD28 induced proliferation by special request.
Test code(s): PWM Blastogenesis, ConA, PHA Blastogenesis, CMV Blastogenesis, HSV Blastogenesis, VZV Blastogenesis, Candida blastogenesis, ADV Blastogenesis, Tetanus Blastogenesis.
Lymphocyte Proliferation to Mitogens. 3H Thymidine incorporation assay measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Concanvalin A (CON A), Pokeweed mitogen (PWM), Interleukin 2 (IL2) and anti-CD3.
Test code(s): SPL3
Lymphocyte Proliferation to Antigens. 3H Thymidine incorporation assay measuring lymphocyte proliferation to the antigens Tetanus/Diphtheria, Candida albicans and alloantigen.
Test code(s): SPL4
CMV-specific CD8 T cell immune competence is evaluated using MHC class I tetramers (flow assay also includes a quantitative component for CMV-specific CD8 T cells for 5 HLA alleles – A1, A2, B7, B8 and B35). Functional component includes CD107a/b expression and IFN-g production of CMV-specific CD8 T cells.
Test code(s): CMVC8; #88826
CD8 T cell degranulation (CD107a) and measurement of IFN-g after CD8 T cell activation with PMA/ionomycin.
Test code(s): GLIC; #89317
Flow based methodology measuring lymphocyte proliferation to an anti-CD3 panel (soluble anti-CD3, aCD3+aCD28, aCD3+ IL-2).
Test code(s): LPA3P; #62205
Flow based methodology measuring lymphocyte proliferation to the antigens Candida albicans and tetanus toxoid.
Test code(s): LPAG; #60592
Flow based methodology measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA) and Pokeweed (PWM).
Test code(s): LPMG; #60591
CFSE-labeled T cells are assessed for their ability to proliferate to cytokines: anti-CD3, anti-CD3/IL-2, anti-CD3/IL-7, anti-CD3/IL-15, anti-CD3/anti-CD28.
Test code(s): TINTL
Flow-based methodology. CFSE-labeled T cells are assessed for their ability to proliferate to mitogens: ConA/IL-2, PHA/IL-2, and anti-CD3 with and without IL-2. Standard screening test for T cell function.
Test code(s): TMITO
Degranulation CD3+CD8+ blasts (cytotoxic CD8 T lymphocytes) by flow cytometry.
Test code(s): Dégranulation des lymphocytes T
T cell proliferation after PHA and anti-CD3 (OKT3) antibody stimulation by flow cytometry.
Test code(s): PHA, OKT3
3H Thymidine incorporation in response to tetanus toxoid (3 concentrations) and Candida.
Test code(s): LCAND, LTET
3H Thymidine incorporation assay to measure lymphocyte proliferation to Phytohemagglutinin (PHA), Concanvalin A (Con A), and Pokeweed (PWM) (3 concentrations each). Available separately with
Test code(s): LPHA, LCONA, LPMW, or together:
NSUH
Lymphocyte proliferation to Antigen uses 3H thymidine incorporation method to measure lymphocyte proliferation to Tetanus toxoid and Candida albicans.
Test code(s): Antigen
Lymphocyte proliferation to Mitogen uses 3H thymidine incorporation method to measure lymphocyte proliferation to PHA, ConA, and PWM.
Test code(s): Mitogen
Candida-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Candida.
Test code(s): 17270
Tetanus-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Tetanus Toxoid.
Test code(s): 17274
Tuberculin PPD-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Tuberculin PPD (TB PPD).
Test code(s): 17680
Mitogen-induced Lymphocyte Proliferation Panel. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Phytohemagglutinin (PHA), Concanavalin A (Con A), Pokeweed Mitogen (PWM). Test available by prior arrangement only.
Test code(s): 91976
Antigen-induced Lymphocyte Proliferation Panel (Candida, Tetanus, TB PPD). Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Candida, Tetanus Toxoid, Tuberculin PPD (TB PPD). Test available by prior arrangement only.
Test code(s): 91977
Mitogen- and Antigen-induced Lymphocyte Proliferation Panel. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Phytohemagglutinin (PHA), Concanavalin A (Con A), Pokeweed Mitogen (PWM), Candida, Tetanus Toxoid, Tuberculin PPD (TB PPD). Test available by prior arrangement only.
Test code(s): 91978
Phytohemagglutinin (PHA)-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Phytohemagglutinin (PHA). Test available by prior arrangement only.
Test code(s): 91980
Pokeweed Mitogen (PWM)-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Pokeweed Mitogen (PWM). Test available by prior arrangement only.
Test code(s): 91981
Concanavalin A (Con A)-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Concanavalin A (Con A). Test available by prior arrangement only.
Test code(s): 91982
Flow-based methodology measuring lymphocyte proliferation to the mitogen Phytohemagglutinin (PHA) and to the antigens Candida Albicans and Tetanus Toxoid.
Test code(s): LPA-1M2A
Flow-based methodology measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Pokeweed (PWM) and Concanavalin A (Con A).
Test code(s): LPA-3M
Flow-based methodology measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Pokeweed (PWM) and Concanavalin A (Con A), and to the antigens Candida Albicans and Tetanus Toxoid.
Test code(s): LPA-3M2A
T cell Function Analysis: PBMC isolation from whole blood, stimulation with various antigens or mitogens, flow cytometry analysis for CD69 upregulation and interferon gamma production.
Test code(s): 403066 and 403065
Intracellular Cytokine Panel: Whole blood is stimulated with PMA and ionomycin. Following stimulation cells are stained with antibodies to identify the cell population (CD4, CD8, NK and NKT cells) and cytokine (interferon gamma, IL4, or TNF) of interest.
Test code(s): 404474
NK CELL FUNCTIONAL TESTS
Evaluation of patient NK cell CD107a surface expression following exposure to K562 cells.
Test code(s): CD107A
Evaluation of CD107a surface expression/degranulation following exposure of NK cells to K562 cells.
Test code(s): CD107a Mobilization (NK Cell Degran)
Spontaneous NK cell cytotoxicity against K562 cells (4h stimulation, Cr51 assay).
Test code(s): NK Function
Spontaneous NK cell cytotoxicity against K562 cells (4h stimulation, Cr51 assay).
Test code(s): SPL1
Enhanced NK cell cytotoxicity with Interleukin 2 stimulation against K562 cells (4h stimulation, Cr51 assay).
Test code(s): SPL2
Flow cytometry assay using CFSE-labeled K562 target cells and 7-AAD incorporation by apoptotic target cells following culture with PBMC’s at various Effector:Target ratios. Test includes evaluation of CD107a surface expression/degranulation following exposure of NK cells to K562 cells.
Test code(s): CYTAPO
Degranulation of NK cells by flow cytometry.
Test code(s): Dégranulation de NK
NSUH
NK function test measures CD107a surface expression after exposing NK cells to K562 cells.
Test code(s): NK function
Natural Killer Cells, Functional. PBMC isolation from whole blood, incubation with fluorescently labeled K562 at various E:T target ratios, following incubation percent lysis is determined by flow cytometry.
Test code(s): 34184
Flow cytometry assay using CFSE-labeled K562 target cells and Sytox Red incorporation by apoptotic target cells following culture with PBMCs at various Effector:Target ratios.
Test code(s): NKCC
BMC isolation from whole blood, incubation with K562 (fluorescently labeled) at various E:T target ratios, following incubation percent lysis is determined by flow cytometry.
Test code(s): 30035
NEUTROPHIL FUNCTIONAL TESTS
Bactericidal Assays and Chemotaxis
Bactericidal assay. Analysis of the ability of isolated neutrophils to clear S. aureus – manual method.
Test code(s): BACT
Neutrophil chemotaxis. Modified Boyden chamber method utilizing peripheral blood and zymosan activated serum as chemotactic agent. Readout is distance migrated in microns.
Test code(s): CTX
DHR/Oxidative Burst
Neutrophil Oxidative Burst Assay (DHR). White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by Semi-Quantitative Flow Cytometry.
Test code(s): 0096657
Neutrophil’s Oxidative Burst, which reports: Neutrophil Oxidative Index (MFI DHR+ / MFI DHR-), and % Responding Polymorphonuclear Cells.
Test code(s): DHR assay
Functional CGD screening assay, using DHR123 loaded neutrophils pre and post stimulation with PMA to induce oxidative burst reaction.
Test code(s): NFUN
Functional CGD screening assay, using DHR123 loaded neutrophils pre and post stimulation with PMA to induce oxidative burst reaction.
Test code(s): NFUN
Classic dihydrorhodamine (DHR) 123 assay.
Test code(s): Neutrophil Oxidative Burst (DHR)
Chronic granulomatous disease diagnostic screening assay for oxidative burst using dihydrorhodamine (DHR) 123.
Test code(s): CGD Flow
Classic dihydrorhodamine (DHR) 123 assay with PMA stimulation.
Test code(s): SPL8
Classic dihydrorhodamine (DHR) 123 assay.
Test code(s): DHR (for both PMA and fMLP stimulants); #62764; DHRP (only for PMA); #62765; DHRF (only for fMLP); #62766.
Neutrophil’s oxidation (DHR) by flow cytometry.
Test code(s): Activité Oxidative des Neutrophiles
Classic dihydrorhodamine (DHR) 123 assay. Offers NBT assay as back up.
Test code(s): DHR; NBT
NSUH
Neutrophil oxidation assay uses dihydrorhodamine (DHR) 123 and PMA. Report includes MFI ratio.
Test code(s): DHR
Neutrophil Function, Oxidative Burst. Classic dihydrorhodamine (DHR) 123 assay.
Test code(s): 37523
Classic dihydrorhodamine (DHR) 123 assay with PMA stimulation.
Test code(s): GOB
Oxidative Burst: Whole blood is stimulated with PMA or buffer alone, Formation of the reactive oxidants during the oxidative burst is monitored by the addition and enzymatic oxidation of a fluorogenic substrate, DHR 123. The level of reactive oxygen radicals is determined by flow cytometry.
Test code(s): 403002
CYTOKINE PANELS, INDIVIDUAL CYTOKINES AND SOLUBLE PROTEINS
Cytokine Panels
Cytokine Panel. Reported: Interleukin 2 receptor (CD25) soluble; Interferon gamma; Interleukin-1beta, 2, 4, 5, 6, 8, 10, 12, 13, 17; TNF-alpha. Quantitative Multiplex Bead Assay.
Test code(s): 0051394
Cytokine Panel, TH1. Reported: Interleukin 2, 12; Interleukin 2 receptor (CD25) soluble; Interferon gamma. Quantitative Multiplex Bead Assay.
Test code(s): 0051408
Cytokine Panel, TH2. Reported: Interleukin 4, 5, 10, 13. Quantitative Multiplex Bead Assay.
Test code(s): 0051518
Cytokine Panel, Monokines. Reported: Interleukin 1beta, 6, 8, TNF alpha. Quantitative Multiplex Bead Assay.
Test code(s): 0051524
Plasma Cytokine Panel. Luminex Multiplex Panel includes IL-1beta, 2, 4, 5, 6, 8, 10; Interferon-gamma; TNF-alpha; GM-CSF.
Test code(s): Cytokines, Plasma or CSF
TH1 Cytokine 4 Plex Panel: Interleukin 2 [IL-2], Interleukin 12 [IL-12p70], Interferon Gamma [IFNg], Tumor Necrosis Factor alpha [TNFa]. Quantitative Multiplex Bead Assay.
Test code(s): PLASMA TH1
TH2 Cytokine 4 Plex Panel: Interleukin 4 [IL-4], Interleukin 5 [IL-5], Interleukin 6 [IL-6], Interleukin 10 [IL-10]. Quantitative Multiplex Bead Assay.
Test code(s): PLASMA TH2P
TH1/TH2 Panel A: Interleukin 2 [IL-2], Interleukin 4 [IL-4], Interleukin 5 [IL-5], Interleukin 6 [IL-6], Interleukin 10 [IL-10], Interleukin 12 [IL-12p70], Interferon Gamma [IFNg], Tumor Necrosis Factor alpha [TNFa]. Quantitative Multiplex Bead Assay.
Test code(s): PLASMA THPNLA
TH1/TH2 Panel B: Interleukin 2 [IL-2], Interleukin 4 [IL-4], Interleukin 5 [IL-5], Interleukin 10 [IL-10], Interferon Gamma [IFNg], Tumor Necrosis Factor alpha [TNFa]. Quantitative Multiplex Bead Assay.
Test code(s): PLASMA THPNLB
Th1/Th2 Panel Includes: Interferon-gamma, Interleukin-13, Interleukin-12p70, Interleukin-10, Interleukin-5, Interleukin-4, and Interleukin-2. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 403025 Serum or 403026 Plasma
Pro-inflammatory Panel Includes: Interleukin-1b, Interleukin-6, Interleukin-8, and TNF-alpha. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 403027 Serum or 403028 Plasma
Individual Cytokines and Soluble Proteins
CXCL9.
Test code(s): CXCL9
S100A12.
Test code(s): S100A12
S100A8/A9 Heterodimer
Test code(s): S100A8/A9 Heterodimer
B-cell Activating Factor.
Test code(s): BAFF
Interferon gamma. Multiplex Bead Assay.
Test code(s): 0051531
Interferon gamma. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1271 Serum or 1221 Plasma
Interleukin 10. Multiplex Bead Assay.
Test code(s): 0051534
Interleukin-10. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1221 Serum or 1222 Plasma
Interleukin 12. Multiplex Bead Assay.
Test code(s): 0051530
Interleukin-12p70. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1213 Serum or 1223 Plasma
Interleukin 13. Multiplex Bead Assay.
Test code(s): 0051535
Interleukin-13. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1214 Serum or 1224 Plasma
Interleukin 17. Multiplex Bead Assay.
Test code(s): 2013115
Interleukin-17. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 40034 Serum or 40033 Plasma
Interleukin-18.
Test code(s): Interleukin–18 (IL-18)
Interleukin 1beta. Multiplex Bead Assay.
Test code(s): 0051536
Interleukin 1 beta. Measured by ELISA.
Test code(s): 1757
Interleukin 1b. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1215 Serum or 1225 Plasma
Interleukin 2. Multiplex Bead Assay.
Test code(s): 0051588
Quest Diagnostics: Interleukin 2. Measured by ELISA.
Test code(s): 34161
Interleukin-2. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 30041 Serum or 1226 Plasma
Interleukin 4. Multiplex Bead Assay.
Test code(s): 0051532
Interleukin-4. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1217 Serum or 1227 Plasma
Interleukin 5. Multiplex Bead Assay.
Test code(s): 0051533
Interleukin-5. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 30042 Serum or 1228 Plasma
Interleukin 6. Multiplex Bead Assay.
Test code(s): 0051537
Interleukin 6. Measured by high sensitivity ELISA.
Test code(s): 34473X
Interleukin-6. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1257 Serum or 1258 Plasma
Interleukin 8. Multiplex Bead Assay.
Test code(s): 0051538
Interleukin-8. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1219 Serum or 1229 Plasma
Neopterin.
Test code(s): Neopterin, Plasma or CSF
Soluble CD163.
Test code(s): Soluble CD163
Soluble Fas Ligand.
Test code(s): Soluble Fas-Ligand (sFasL)
ALPS- Plasma FAS Ligand/CD95 (ELISA).
Test code(s): Dosage du FAS LIGAND
Interleukin 2 Receptor (CD25), Soluble. By Quantitative Multiplex Bead Assay.
Test code(s): 0051529
2 Receptor (CD25), Soluble.
Test code(s): Soluble IL-2R (Soluble CD25)
Interleukin-2 Receptor EIA. Soluble IL2R levels measured by EIA.
Test code(s): 34298
sIl-2R. The assay employs a sandwich immunoassay format.
Test code(s): 30057
Tumor Necrosis Factor – alpha. Multiplex Bead Assay.
Test code(s): 0051539
TNF-alpha. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1220 Serum or 1230 Plasma
COMPLEMENT TESTS (INCLUDING INDIVIDUAL COMPONENTS, FUNCTIONAL ASSAYS, AND SPLIT PRODUCTS) - GROUPED BY SPECIALTY LAB
Complement, Alternative Pathway (AH50), Functional Serum (separately orderable).
Test code(s): AH50; #88676
Atypical Hemolytic Uremic Syndrome (aHUS) Complement Panel.
Test code(s): AHUSD; #64881
C1 Esterase (C1ES) Inhibitor Antigen, Serum (nephelometry).
Test code(s): C1ES; #8198
Complement C1q, Serum (nephelometry).
Test code(s): C1Q; #8851
C1q Complement Functional, Serum (Automated Liposome Lysis [ALL]).
Test code(s): C1QFX; #83374
C2 Complement Functional, Serum (ALL).
Test code(s): C2; #81835
C2 Complement Antigen, Serum (RID – reflex testing only).
Test code(s): C2AG; #84141
C2 Complement Functional (no reflex, ALL).
Test code(s): C2FXN; #32137
Complement C3, Serum (nephelometry).
Test code(s): C3; #8174
C3 Functional, Serum (ALL).
Test code(s): C3FX; #81090
Complement C4, Serum (nephelometry).
Test code(s): C4; #88171
C4d Complement Activation Fragment, Plasma (AHUSD only).
Test code(s): C4D; #62584
C4 Functional, Serum (ALL).
Test code(s): C4FX; #83391
C5 Complement, Antigen, Serum (nephelometry).
Test code(s): C5AG; #9266
C5 Functional, Serum (ALL).
Test code(s): C5FX; #83392
C6 Functional, Serum (ALL
Test code(s): C6FX; #83393
C7 Functional, Serum (ALL).
Test code(s): C7FX; #81064
C8 Functional, Serum (ALL).
Test code(s): C8FX; #81065
C9 Functional, Serum (ALL).
Test code(s): C9FX; #81066
CBB Complement Activation Fragment, Plasma (AHUSD only).
Test code(s): CBB; #62585
Complement, Total, Serum.
Test code(s): COM; #8167
Factor B Complement Antigen (AHUSD only, nephelometry).
Test code(s): FBCA; #62588
C1 Esterase Inhibitor Functional Assay, Serum (ELISA).
Test code(s): FC1EQ; #81493
Factor H Complement Antigen (AHUSD only, nephelometry).
Test code(s): FHCA; #62587
SC5b-9 Complement Activation Complex, Plasma (AHUSD only).
Test code(s): SC5B9; # 62586
C1 Esterase Inhibitor Functional.
Test code(s): 1112
C1 Esterase Inhibitor Protein.
Test code(s): 1113
C1q Complement.
Test code(s): 1115
Mannan Binding Lectin (MBL).
Test code(s): 33
Mannan-Binding Lectin Pathway Function Test (C4b).
Test code(s): 34
C4 Complement.
Test code(s): 8
Autoantibodies to Individual Components
Immune Complex Panel (by Flow and ELISA).
Test code(s): 0050667
Autoantibody to C1q (C1q-CLR, by ELISA).
Test code(s): C1QAB
C3 Nephritic factor (by Immunofixation Electrophoresis).
Test code(s): C3NEF
Autoantibody to C1-inhibitor by (ELISA).
Test code(s): CEIAP
Autoantibody to Factor H (by ELISA).
Test code(s): FHAB
Component Levels
C1-Esterase Inhibitor Panel (by ELISA, Turb and Neph).
Test code(s): 0050139
C1-Esterase Inhibitory (by Quant Neph).
Test code(s): 0050140
Complement Component 2 (by RID).
Test code(s): 0050148
Complement Components 3 and 4 (by Quant. Immunoturbid).
Test code(s): 0050149
Complement Component 3 (by Quant. Immunoturbidimetry).
Test code(s): 0050150
Complement Component 4 (by Quant. Immunoturbidimetry).
Test code(s): 0050155
Complement Component 5 (by RID).
Test code(s): 0050156
Complement Factor B (by RID).
Test code(s): 0051720
Complement Component 1Q Level (by RID).
Test code(s): 0099130
C1q level (by RID).
Test code(s): C1Q
C1r level (by RID).
Test code(s): C1RL
C1s level (by RID).
Test code(s): C1SL
C2 level (by RID).
Test code(s): C2L
C3 level (by nephelometry).
Test code(s): C3
C4 level (by nephelometry).
Test code(s): C4
C5 level (by RID).
Test code(s): C5L
C6 level (by RID).
Test code(s): C6L
C7 level (by RID).
Test code(s): C7L
C8 level (by RID).
Test code(s): C8L
C9 level (by RID).
Test code(s): C9L
C1-esterase inhibitor level (C1-INH, by RID).
Test code(s): CEIQ
Circulating immune complexes (C1q-binding and C3d).
Test code(s): CIC
Factor B level (by RID).
Test code(s): FBL
Factor D level (by ELISA).
Test code(s): FDL
Factor H level (by RID).
Test code(s): FH
Factor I level (by RID).
Test code(s): FIL
Mannose binding lectin (by ELISA).
Test code(s): MLEC
Properdin level (by ELISA).
Test code(s): PROP
Mannose binding lectin (by ELISA).
Test code(s): 16039X
C1-esterase inhibitor protein (quantitative, by EIA).
Test code(s): 298
Properdin Factor B (by nephelometry).
Test code(s): 300
C7 level (by RID).
Test code(s): 34888X
C8 level (by RID).
Test code(s): 34889X
C9 level (by RID).
Test code(s): 34896
C3 level (by nephelometry).
Test code(s): 351
C4 level (by nephelometry).
Test code(s): 353
C5 level (by RID).
Test code(s): 354
Circulating immune complexes (C1q-binding, by ELISA).
Test code(s): 36735
C2 level (by RID).
Test code(s): 433X
C6 level (by RID).
Test code(s): 464
C1q level (by RID).
Test code(s): 981X
Functional Assays
C1-Esterase Inhibitor Functional (by ELISA).
Test code(s): 0050141
Complement Activity Enzyme Immunoassay, Total (by ELISA).
Test code(s): 0050198
C1q Binding Assay (by ELISA).
Test code(s): 0050301
Complement Activity, Alternative Pathway (AH50, by RID).
Test code(s): 2005373
C1 function (by hemolytic assay).
Test code(s): C1F
C1q function (by hemolytic assay).
Test code(s): C1QH
C2 function (by hemolytic assay).
Test code(s): C2F
C3 function (by hemolytic assay).
Test code(s): C3F
C4 function (by hemolytic assay).
Test code(s): C4F
Rapid screen for deficiency of late components (C5, C6, C7, C8, C9, CH50).
Test code(s): C59S
C5 function (by hemolytic assay).
Test code(s): C5F
C6 function (by hemolytic assay).
Test code(s): C6F
C7 function (by hemolytic assay).
Test code(s): C7F
C8 function (by hemolytic assay).
Test code(s): C8F
C9 function (by hemolytic assay).
Test code(s): C9F
C1 esterase inhibitor function, Chromogenic.
Test code(s): CEICHR
Factor D function (by hemolytic assay).
Test code(s): FDF
Factor B function (by hemolytic assay).
Test code(s): PFBF
C1-esterase inhibitor protein (functional, by EIA).
Test code(s): 297
Individual Complement Split Products
Bb level (by ELISA).
Test code(s): BBLL
C3a desArg level (by RIA).
Test code(s): C3AL
C4a desArg level (by RIA).
Test code(s): C4AL
C4d level (by ELISA).
Test code(s): C4D
Ratio of C4d to C4.
Test code(s): C4RAT
C5a desArg level (by RIA).
Test code(s): C5AL
iC3b level (by ELISA).
Test code(s): IC3B
SC5b-9 level (by ELISA).
Test code(s): SC5B9
C3a desarg (by ELISA).
Test code(s): 17689X
C3d fragment (by EIA).
Test code(s): 4943N
OTHER SPECIALIZED TESTS
Autoantibodies to Cytokines: Detection and Functional Assays
Measurement of STAT5 phosphorylation following GMCSF stimulation.
Test code(s): GM-CSF Receptor Stimulation
Measurement of autoantibodies to GMCSF by ELISA.
Test code(s): GMCSF Autoantibody
Measures of phosphorylation/dephosphorylation STAT5 (IL2, IL3, IL15, and GMCSF-induced) by flow cytometry.
Test code(s): Étude functionnelle and indicate yourself
ELISA measurement of GM-CSF autoantibodies, and reflex testing of P-STAT5 inhibition for positive samples.
Test code(s): GMCSFA
Measures of phosphorylation of STAT1 in response to gIFN by flow cytometry.
Test code(s): Étude functionnelle and indicate yourself
Binding and functional analysis of autoantibodies to IFNg by ELISA and reflex P-STAT1 inhibition assay.
Test code(s): IFNGAB
Molecular Tests (non-Gene Sequencing)
Both visual interpretation of spectratype and diversity ratio information included in report.
Test code(s): TCRVB; #62930
T-cell Receptor (TCR) Gamma Gene Rearrangement, PCR. Interrogates the T-cell receptor gamma locus (TCRG) by a PCR method based on the BIOMED-2 consensus.
Test code(s): 90509
T-cell Clonality Panel (TCRG, TCRB), PCR. Interrogates both the T-cell receptor beta (TCRB) locus and the T-cell receptor gamma (TCRG) locus by a PCR method based on the BIOMED-2 consensus.
Test code(s): 91445
T-cell Receptor (TCR) Beta Gene Rearrangement, PCR. Interrogates the T-cell receptor beta locus (TCRB) by a PCR method based on the BIOMED-2 consensus.
Test code(s): 91446
TREC quantitatoin reported as copies per million CD3+ T cells.
Test code(s): TREC; #87959
Specialized Serologic Testing for Vaccine Response Evaluation (not including routine vaccine titers)
Functional Antibody Analysis- Pneumococcal Avidity. A modification of the standard pneumococcal assay, using varying concentrations of ammonium thiocyanate. Bound antibodies are detected with an antibody that recognizes human IgG. The IgG antibody concentration is calculated using a standard curve referenced to WHO standard serum 89SF. The avidity is calculated based on binding with various concentrations of thiocyanate. Multiple Panels available with varying number of serotypes:
Test code(s): 401734, 401846, 401839, and 4017345
Therapeutic Monitoring Lymphocyte Depletion
Flow based assay to measure alemtuzumab levels in whole blood.
Test code(s): Alemtuzumab Level
Quantifies CD52 on T cells, NK cells, B cells, monocytes.
Test code(s): CD52 Expression
