Clinical Immunology Society

DLI Lab Directory Search

GENERAL IMMUNOPHENOTYPING (FLOW CYTOMETRY)

CD4+ Recent Thymic Emigrants

ARUP
CD4+ T-Cell Recent Thymic Emigrants (RTEs). Reports percentage and absolute numbers of CD4+CD31+CD45RA+ (RTEs). By Quantitative Flow Cytometry.
Test code(s): 2010179
BCH-CFCL
Immunophenotyping of Recent Thymic Emigrants, which reports: CD4+CD31+CD45RA+Recent Thymic Emigrants.
Test code(s): RTE Panel
CALGARY-CLS
Reports the % and absolute count of CD3+CD4+CD45RA+CD31+ recent thymic emigrants.
Test code(s): RTE
CCHMC-DIL
CD45RA/RO panel includes evaluation of CD31 expression as a marker of recent thymic emigrants.
Test code(s): CD45RA/RO
CHLA-DIFL
The percentages and absolute numbers of lymphocytes that are newly formed CD4+ cells with following phenotypes of CD4+CD45+RA+ cells: CD3+4+/RA+RO+, CD3+4+/RA+/CD31+ and CD3+4+/RA+62L+ are reported.
Test code(s): T Cell Subsets (Memory and Naïve)
MAYO-ML
Reports the percentage of CD4+ 45RA+ CD31+ T cells.
Test code(s): CD4RT; #89504
MONTREAL-SJH
Naïve/memory T lymphocyte subsets: CD4+CD45RO+, CD4+CD45RA+, CD4+CD45RA+ CD31+, and CD8+ CD45RA+ CCR7+ (naïve), CD8+CD45RA-CCR7+ (CM), CD8+CD45RA-CCR7- (TEM), CD8+CD45RA+CCR7- (TEMRA).
Test code(s): CD45RA/CD4, CD45RA-31/CD4, CD45RO/CD4, CD45RA/CD8, CD45RA-31/CD8, CD45RO/CD8
NJH-ADL
Identifies RTEs using a 4-color panel: CD3/CD4/CD45RA/CD31. Reports percentages.
Test code(s): CD4RTE

Lymphocyte Subset Enumeration

ARUP
Natural Killer Cells Enumeration: The results reported are the percentage of lymphocytes that are Natural killer (NK) cells and the absolute count of NK-cells per µL of blood. Only those cells that express CD45 and either CD16 or CD56 (or both) but not CD3 or CD19 are reported. By Quantitative Flow Cytometry.
Test code(s): 0092404
Lymphocyte Subset Panel 3: T-Cell Subsets (CD4 and CD8), Absolute Counts Only. By Quantitative Flow Cytometry. Reported; Abs CD3, CD4, CD8, and abs CD4:CD8 ratio.
Test code(s): 0095853
Lymphocyte Subset Panel 1: CD4 Absolute Count Only. Reported; abs CD4. By Quantitative Flow Cytometry.
Test code(s): 0095854
Lymphocyte Subset Panel 2: CD4 Percent and Absolute. Reported; % CD4, and Abs CD4. By Quantitative Flow Cytometry.
Test code(s): 0095885
Lymphocyte Subset Panel 5: Total Lymphocyte Enumeration. Reported; % CD3, abs CD3, % CD4, abs CD4, % CD8, abs CD8, CD4: CD8 ratio, % CD19, abs CD19, % NK-cells, abs NK-cells. By Quantitative Flow Cytometry.
Test code(s): 0095892
Isolated Panel for T cells (Monitoring post ATG): Lymphocyte Transplantation CD3. Reported: WBC (K/µL), % Lymph, Absolute Lymph Count, %CD3, abs CD3. By Quantitative Flow Cytometry.
Test code(s): 0095949
Lymphocyte Subset Panel 4: T-Cell Subsets Percent and Absolute. Reported; %CD3, abs CD3, %CD4, abs CD4, %CD8, abs CD8, CD4:CD8 Ratio. By Quantitative Flow Cytometry.
Test code(s): 0095950
BCH-CFCL
Analysis of lymphocytes populations, which reports: The percentages and absolute numbers of lymphocytes that are CD3+ (Total T cells), CD3+CD4+ (T helper cells), CD3+CD8+ (T cytotoxic cells), CD4:CD8 ratio, CD3-CD19+ (B cells), CD-CD16/56+ (NK cells).
Test code(s): Lymphocyte Subsets or T/B/NK Panel
CALGARY-CLS
Immunodeficiency screen panel includes percentages and absolute numbers of T cells, B cells, NK cells, TNK (CD3+16/56+), CD3+4+, CD3+8+, DNT (CD3+4-8-), CD4/CD8 ratio, Naïve B cells (CD19+IgD+CD27-), Memory non-class switched B cells (CD19+IgD+CD27+), Memory class switched B cells (CD19+IgD-CD27+), Naïve Helper T cells (CD3+4+CD45RA+CD27+), Terminally Differentiated Helper T cell (CD3+4+45RA+27-), Central Memory Helper T cell (CD3+4+45RA-27+), Effector Memory Helper T cell (CD3+4+45RA-27-), Naïve Cytotoxic T cells (CD3+8+CD45RA+CD27+), Terminally Differentiated Cytotoxic T cell cell (CD3+8+45RA+27-), Central Memory Cytotoxic T cell (CD3+8+45RA-27+), Effector Memory Cytotoxic T cell (CD3+8+45RA-27-). CD45RA/CD45RO also assessed.
Test code(s): IDEF
CCHMC-DIL
The percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+) are reported.
Test code(s): Lymphocyte Subsets
CHLA-DIFL
T cell Extended panel: The percentages and absolute numbers of lymphocytes that are CD3+ (Total T cells), CD3+CD4+ (T helper cells), CD3+CD8+ (T cytotoxic cells), CD4:CD8 ratio, CD3-CD19+ (B cells), CD-CD16/56+ (Natural Killer (NK) cells), and CD3+ HLA-DR+ (Activated T cells) are reported.
Test code(s): T-Cell Extended
T cell Standard panel: The percentages and absolute numbers of lymphocytes that are CD3+ (Total T cells), CD3+CD4+ (T helper cells), CD3+CD8+ (T cytotoxic cells), CD4:CD8 ratio, and CD3+ HLA-DR+ (Activated T cells) are reported.
Test code(s): T-Cell Standard
MAYO-ML
The percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+) are reported.
Test code(s): TBBS; #9336
MCW-CIRL
CD3, CD4, CD8, CD14, CD45.
Test code(s): AT4
CD3, CD4, CD8, CD16, CD56, CD19, CD14, CD15, CD11b, CD45.
Test code(s): PID1
MONTREAL-SJH
Lymphocyte subset phenotyping by quantitative flow cytometry: B lymphocyte subsets CD19+, CD19+CD27+, CD19+CD20+.
Test code(s): CD19, CD27/CD19, CD20
Lymphocyte subset phenotyping by quantitative flow cytometry: NK cells: CD3-CD56+.
Test code(s): CD3 (-) CD56 (+)
Lymphocyte subset phenotyping by quantitative flow cytometry: T lymphocytes: CD3+, CD3+CD4+, CD3+CD8.
Test code(s): CD3, CD4, CD8
NJH-ADL
CD3/4/8: The percentages and absolute numbers are reported.
Test code(s): CD3SB
CD4/8: The percentages and absolute numbers are reported.
Test code(s): CD48
Individual markers - CD4: The percentage and absolute number are reported.
Test code(s): CD4M
Individual markers - CD16/56: The percentage and absolute number are reported.
Test code(s): NKMK
Lymphocyte Phenotyping Panel: CD3+, CD3+/CD4+, CD3+/CD8+, CD16/56 and CD19+. CD4/CD8 ratio reported. The percentages and absolute numbers are reported.
Test code(s): TBCDC
CD3/4/8/19: The percentages and absolute numbers are reported.
Test code(s): TBSBS
NSUH
Lymphocyte subset uses CD3, CD4, CD8, CD19, CD16, and CD56. Report includes percentages and absolute counts. CD4 to CD8 ratio is also reported.
Test code(s): TnB
UCLA-UIC
Both the T-cell memory and the B-cell panels include a basic panel for lymphocyte enumeration, which reports the percentages and absolute numbers of T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), CD4:CD8 ratio, natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+), followed by an advanced panel to identify (i) T-memory: naïve (CD45RA+/CD45RO-) and Memory (CD45RA-/CD45RO+) CD4+ and CD8+ T cells; (ii) B-cell: Memory (CD27+), IgM Memory (CD27+IgM+IgD-), Unswitched Memory (CD27+IgM+IgD+), Switched Memory (CD19+CD27+IgD-IgM-) and Immature (CD21low) CD19+ B cells.
Test code(s): T-MEM or B-CELL

Lymphocyte/T cell Activation

CALGARY-CLS
Panel 1: CD3+4+ T cells are assessed for surface expression of CD154, CD69, HLA-DR, CD143, CD25, CD71, and CD96 pre and post PMA/Ionomycin stimulation. Panel 2: CD8+ T cells are assessed for expression of CD45RO and HLA-DR primarily for use in investigation of Omenn’s Syndrome or HLH. Antigen density of CD5 on CD3+8+ cells is also reported for HLH investigations.
Test code(s): LAM
CCHMC-DIL
Measures CD4+ T cell surface expression of CD69, CD25, CD154, CD134, CD95, CD71, and HLA-DR.
Test code(s): Lymphocyte Activation Markers
CHLA-DIFL
T Cell Activation Panel. The following populations are reported in percentages: CD25+CD3+/CD3, DR+CD3+/CD3, CD69+CD3+/CD3, CD134+CD3+/CD3, CD25+CD4+/CD3, DR+CD4+/CD3, CD69+CD4+/CD3, CD134+CD4+/CD3.
Test code(s): T Cell Subsets Activated T
MAYO-ML
The T cell phenotyping assay includes evaluation of activated (CD4+25+ and MHC class II-positive) CD4+ and CD8+ T cells.
Test code(s): TCP; #89319
MCW-CIRL
T cell activation panel: Includes CD4, CD8, CD69, and CD25 at 4 hours and 4 days after activation to measure early and late activation events:
Test code(s): TCACT
MONTREAL-SJH
Sainte Justine Hospital: Percentages of activated T cells for HLA class II DR expression on CD3+, CD3+CD4+, and CD3+CD8+ by flow cytometry.
Test code(s): HLA class II/CD3, HLA class II/CD4, HLA class II/CD8

NK Cell Phenotyping

ARUP
Natural Killer Cells Enumeration. The results reported are the percentage of lymphocytes that are Natural killer (NK) cells and the absolute count of NK-cells per µL of blood. Only those cells that express CD45 and either CD16 or CD56 (or both) but not CD3 or CD19 are reported. By Quantitative Flow Cytometry.
Test code(s): 0092404
Natural Killer Cell and Natural Killer T-Cell Panel. The panel reports frequencies and absolute cell counts of NK and NKT-cells, also including T and non-T cells: CD3-CD16-/+CD56bright/dim (total NK-cells), CD3-CD16+CD56dim (cytotoxic NK-cells), CD3-CD16-CD56bright (cytokine secreting NK-cells), CD3-CD57+ (CD57+NK-cells), CD3+CD56+ (CD56+ NKT-cells), CD3+CD57+ (CD57+ NKT-cells), CD45+CD3+ (T-cells) and CD45+CD3- (Non T-cells).
Test code(s): 2013805
BCH-CFCL
Immunophenotyping of Natural Killer cells which reports: percentages of CD56 high NK cells, CD56+CD16+ NK cells, and CD56-CD16 + NK cells.
Test code(s): NK Cell Phenotype
MAYO-ML
Includes quantitation of cytotoxic and cytokine-producing NK cells based on expression and intensities of CD16 and CD56, reports CD3+ CD56+.T cells, and includes CD69, CD27, NKp46, NKG2D, CD95 CD107a/b, Perforin, Granzyme A, Granzyme B, and IFNg. Test Codes: QNKS; #60616 (for only the major NK/NKT cell subset quant without intracellular and surface markers; NKSP (for complete panel) #60615
Test code(s):
MCW-CIRL
NK panel includes: CD3, CD4, CD8, CD56, Perforin, Granzyme A, Granzyme B, CD19, CD14, CD15, CD11b, CD45.
Test code(s): PERGRA

NKT cell Subsets (including invariant NKT cells)

CALGARY-CLS
The percentage and absolute count of CD3+ TCRvalpha24+ TCRvbeta11+ iNKT cells is reported.
Test code(s): INKT
CCHMC-DIL
Quantification of TCRValpa24 and TCR Vbeta 11 expression on invariant NKT cells.
Test code(s): iNKT
MONTREAL-SJH
Quantification of MAIT (Va 24 Vb 11) and iNKT (Va 24 Vb 11) cells.
Test code(s): NKT

Regulatory T Cell Analysis and/or FOXP3 Expression

ARUP
Regulatory T-Cell Panel. Reported: Percent and absolute numbers of CD4+ CD25+ CD127Low (Tregs), and CD45RA and CD45RO also included in the panel. By Quantitative Flow Cytometry.
Test code(s): 2010172
BCH-CFCL
Immunophenotyping of Regulatory T cells, which reports: CD4+CD25+CD127+ T cells, CD4+CD25highCD127low T cells.
Test code(s): Treg Panel
CALGARY-CLS
Reports the % and absolute count of Tregs determined by CD3+ CD4+ lowCD127+ and CD25+.
Test code(s): TREG
CCHMC-DIL
Quantifies the percent of T regs (CD25+ CD127 lo) as a percentage of the CD4+ T cells, and quantifies the percentage of T regs, which express FOXP3.
Test code(s): FoxP3
CHLA-DIFL
Regulatory T-Cell (T Reg). Percent of CD4+ CD25+ CD127dim is reported.
Test code(s):
MAYO-ML
Identifies percentages and absolute numbers of CD4+CD25+CD127loCD45RO+ (Nat Tregs) and CD4+CD25+CD127loCD45RA+ Naive Tregs.
Test code(s): TREGS; #89318
MCW-CIRL
Treg panel includes: CD4, CD25, CD127, FoxP3.
Test code(s): TREG
MONTREAL-SJH
Percentages of regulatory T cells: CD4+ CD25high CD127- Foxp3+ by flow cytometry.
Test code(s): FOXp3
NJH-ADL
National Jewish Health using a 4-color panel, CD3/4/CD25/ FoxP3, gating on CD25highFoxP3+ cells. Reports percentages.
Test code(s): FOXP3
NSUH
Regulatory T-Lymphocytes uses CD4, CD25, CD127, and FoxP3. Percentages are reported.
Test code(s): FoxP3

T Cell Phenotyping (Naïve T Cells, Memory T Cells, Extended Phenotyping)

ARUP
Lymphocyte Subset Panel 6: Total Lymphocyte Enumeration with CD45RA and CD45RO. Reported; %CD3, abs CD3, % CD4, abs CD4, % CD45RA, abs CD45RA, % CD45RO, abs CD45RO, % CD8, abs CD8, CD4:CD8 Ratio, % CD19, abs CD19, % NK-cells, abs NK-cells. By Quantitative Flow Cytometry.
Test code(s): 0095862
Lymphocyte Subset Panel 7: Congenital Immunodeficiencies. Reported; %CD2, abs CD2, % CD3, Abs CD3, %HLA-DR, abs HLA-DR, % CD4, abs CD4, % CD45RA, Abs CD45RA, % CD8, abs CD8, CD4: CD8 ratio, % CD19, abs CD19, % NK-cells, abs NK-cells. By Quantitative Flow Cytometry.
Test code(s): 0095899
BCH-CFCL
Immunophenotyping of Naïve and Memory T cells, which reports: TEMRA CD4+CD45RA+CCR7-T cells, Naïve CD4+CD45RA+CCR7+T cells, Effector memory CD4+CD45RA-CCR7-T cells, Central memory CD4+CD45RA-CCR7+T cells, TEMRA CD8+CD45RA+CCR7-T cells, Naïve CD8+CD45RA+CCR7+T cells, Effector memory CD8+CD45RA-CCR7-T cells, Central memory CD8+CD45RA-CCR7+T cells.
Test code(s): T Cell Memory Panel
CALGARY-CLS
Immunodeficiency screen panel includes percentages and absolute numbers of T cells, B cells, NK cells, TNK (CD3+16/56+), CD3+4+, CD3+8+, DNT (CD3+4-8-), CD4/CD8 ratio, Naïve B cells (CD19+IgD+CD27-), Memory non-class switched B cells (CD19+IgD+CD27+), Memory class switched B cells (CD19+IgD-CD27+), Naïve Helper T cells (CD3+4+CD45RA+CD27+), Terminally Differentiated Helper T cell (CD3+4+45RA+27-), Central Memory Helper T cell (CD3+4+45RA-27+), Effector Memory Helper T cell (CD3+4+45RA-27-), Naïve Cytotoxic T cells (CD3+8+CD45RA+CD27+), Terminally Differentiated Cytotoxic T cell (CD3+8+45RA+27-), Central Memory Cytotoxic T cell (CD3+8+45RA-27+), Effector Memory Cytotoxic T cell (CD3+8+45RA-27-). CD45RA/CD45RO also assessed.
Test code(s): IDEF
CHLA-DIFL
Naïve T Cell Subsets. The percentages and absolute numbers of lymphocytes that are newly formed CD4+ cells with following phenotypes of CD4+CD45+RA+ cells: CD3+4+/RA+RO+, CD3+4+/RA+/CD31+ and CD3+4+/RA+62L+ are reported.
Test code(s): T cell Subsets (Memory and Naïve)
MAYO-ML
Quantitates naïve (global and CD62L+), memory (global, central, and effector memory), and activated (CD4+25+ and MHC class II-positive) CD4+ and CD8+ T cells using antibodies directed against CD3, CD4, CD8, CD45RO, CD45RA, HLADR, CD27, CD62L, CD25, and CD28.
Test code(s): TCP; #89319
MCW-CIRL
CVID Panel: CD3, CD4, CD8, CD16, CD56, CD19, CD21, CD27, IgD, HLA-DR, CD45RA, CD45RO, CD69, CD14, CD15, CD11b, CD45.
Test code(s): CVID
Primary Immunodeficiency 2 panel includes CD3, CD4, CD8, TCRαβ, CD69, CD45RA, CD45RO, CD16, CD56; CD19, CD20, CD27, CD5, CD14, CD15, CD11b, CD18, CD45, HLA-DR, HLA-ABC.
Test code(s): PID2
MONTREAL-SJH
Naïve/memory T lymphocytes: CD4+CD45RO+, CD4+CD45RA+, CD4+CD45RA+CD31+/ CD8+CD45RA+CCR7+ (naïve), CD8+CD45RA-CCR7+ (CM), CD8+CD45RA-CCR7- (TEM), CD8+CD45RA+CCR7- (TEMRA).
Test code(s): CD45RA/CD4, CD45RA-31/CD4, CD45RO/CD4, CD45RA/CD8, CD45RA-31/CD8, CD45RO/CD8
NJH-ADL
National Jewish Health runs a memory and naïve T cell panel that includes CD3/4/CD45RA/CD45RO.
Test code(s): C4RARO
NSUH
Naïve/memory T-Lymphocyte subset uses CD3, CD4, CD8, CD45RA, and CD45RO. Percentages are reported.
Test code(s): CD45RARO
UCLA-UIC
The T-cell memory panel includes a basic panel for lymphocyte enumeration, which reports the percentages and absolute numbers of T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), CD4:CD8 ratio, natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+), followed by an advanced panel to identify naïve (CD45RA+/CD45RO-) and Memory (CD45RA-/CD45RO+) CD4+ and CD8+ T cells.
Test code(s): T-MEM

TCR V Beta Repertoire

ARUP
T-Cell Clonality by Flow Cytometry Analysis of TCR V-beta. Quantitates 24 alpha-beta T cell antigen receptors.
Test code(s): 0093199
CALGARY-CLS
Provides % and graphic representation of 24 vbeta repertoire antigens on CD3, CD3+4+ and CD3+8+ subsets.
Test code(s): TCR FLOW
CCHMC-DIL
Quantitates 24 antigens of the TCR V β repertoire of T lymphocytes.
Test code(s): TCR V Beta Repertoire
CHLA-DIFL
TCR V Beta Repertoire. Quantitates 24 V Beta TCR family receptors in total CD3, CD4, and CD8 T-cell subsets.
Test code(s):
MAYO-ML
See Molecular Tests: TCR Vb Spectratyping
Test code(s):
MONTREAL-SJH
Percentages of TCR Vb repertoire (flow cytometry) in CD4+ and CD8+ T cells. Test Code: V Beta 2/CD4, V Beta 2/CD8, 6 V Beta /CD4, 6 V Beta /CD4
Test code(s):
QUEST
See Molecular Tests: TCR Vb Spectratyping
Test code(s):

TCR α/β - TCR γ/δ

BCH-CFCL
Immunophenotyping of γδ and CD4-CD8- αβ T cells, which reports: CD3+CD4-CD8-TCR alpha/beta T cells, CD3+TCR gamma/delta T cells. See also ALPS panel.
Test code(s): ALPS Panel
CALGARY-CLS
Reports the % and absolute count of TCRα/β and TCRγ/δ positive T cells:
Test code(s): TCRABGD
CCHMC-DIL
Quantifies the percentages of T cells expression α/β TCR and γ/δ TCR.
Test code(s): TCR α/β TCR γ/δ
CHLA-DIFL
Quantifies the percentages of T cells expression α/β TCR and γ/δ TCR.
Test code(s): TCR α/β TCR γ/δ
MONTREAL-SJH
Percentages of TCR α/β /CD3 and TCR γ/δ /CD3.
Test code(s): TCR α/β TCR γ/δ /CD3
NJH-ADL
Quantifies the percentages of TCRα/β and γ/δ T cells.
Test code(s): TCABGD
NSUH
T-lymphocyte TCR subset uses CD3, CD4, CD8, TCRα/β, and TCRγ/δ. Percentages are reported.
Test code(s): TCR

DISEASE-SPECIFIC FLOW CYTOMETRY TESTS OR PANELS

ALPS Panel

ARUP
Autoimmune Lymphoproliferative Profile. Reported; % Alpha/Beta+, CD4-, CD8-, Absolute Alpha/Beta+, CD4-, CD8-, % CD5+, CD20+, Absolute CD5+, CD20+, % CD3+, HLA-DR+, Absolute CD3+, HLA-DR+. By Quantitative Flow Cytometry.
Test code(s): 0095505
BCH-CFCL
Immunophenotyping of γδ and CD4-CD8- αβ T cells, which reports: CD3+CD4-CD8-TCR alpha/beta T cells, CD3+TCR gamma/delta T cells.
Test code(s): ALPS Panel
CALGARY-CLS
The % and absolute count of CD3, CD3+4-8-TCRαβ+ DNT cells are reported as well as the % of DNT that express CD95, TCRαβ, HLA-DR and CD45R (B220). Additional antibodies to CD45R, CD25, HLA-DR CD19, and CD27 are used in an ALPS scoring system.
Test code(s): ALPS
CCHMC-DIL
ALPS panel uses antibodies against CD3, CD4, CD8, CD45, alpha beta TCR, CD25, CD27, HLA-DR, CD19, CD5, CD27, and B220 to create a panel that quantifies double negative T cells, CD27+ B cells, and CD25 and HLA-DR expression on T cells to rank the likelihood of ALPS.
Test code(s): ALPS Panel by Flow
CHLA-DIFL
ALPS Panel Reports % CD3+CD4-CD8-αβ+, % CD3+CD4-CD8-γδ+, and %CD3+αβ+, %CD3+γδ+.
Test code(s): ALPS Panel Flow
MAYO-ML
Uses antibodies against CD3, CD4, CD8, CD45, alpha beta TCR, and B220 to quantify Double Negative T Cells, analyze B220 expression.
Test code(s): ALPS; #82449
MCW-CIRL
Autoimmune Lymphoproliferative Syndrome Panel includes CD3, CD4, CD8, CD16, CD56, CD19, CD14, CD15, CD11b, CD45, CD21, CD27, IgD, TCRαβ, B220, HLA-DR, CD25.
Test code(s): AILYMP
MONTREAL-SJH
CD3+CD4−CD8−/TCRαβ double negative T cells enumeration.
Test code(s): CD4(-)CD8(-)/ TCRαβ
NJH-ADL
Reports percentages of DNTs using a CD3/CD48/TCRαβ panel.
Test code(s): ALPS
NSUH
T-lymphocyte TCR subset uses CD3, CD4, CD8, TCRα/β, TCR γ/δ, B220, CD20, CD5, and CD45. Percentages are reported.
Test code(s): ALPS

BAFF-R and TACI Expression on B cells

MAYO-ML
Reports the percent of CD19+ B cells expressing TACI and BAFF-R.
Test code(s): CVID; #87993
NJH-ADL
Reports BAFF-R qualitatively as present/absent and includes commentary comparing the expression level to that of a date matched normal control.
Test code(s): BAFFR

BTK Protein Flow

ARUP
Bruton Tyrosine Kinase (BTK) Protein Expression by Flow Cytometry (Qualitative Flow Cytometry).
Test code(s): 2012002
CALGARY-CLS
Bruton Tyrosine Kinase (BTK) Protein Expression by Flow Cytometry in B cells and monocytes.
Test code(s): BTK
MAYO-ML
Analyzes BTK expression in B cells and monocytes.
Test code(s): BTK; #89011
MCW-CIRL
Analyzes BTK expression in B cells and monocytes.
Test code(s): BTK

CD18, CD11a/CD11b, and CD15s Expression (Screening for LAD)

ARUP
Leukocyte Adhesion Deficiency Panel. Panel measures CD11b, CD15, and CD18 on neutrophils. By Semi-Quantitative Flow Cytometry.
Test code(s): 2004359
BCH-CFCL
Leukocyte Adhesion Deficiency, which reports: CD18+ cells, CD15s+ cells.
Test code(s): LAD Panel
CALGARY-CLS
Measures expression of CD18, CD11a, CD11b, CD11c (Type I LAD) and CD15s (Type II LAD) on the surface of granulocytes.
Test code(s): LAD
CCHMC-DIL
Measures CD18 and CD11b expression on neutrophils.
Test code(s): Neutrophil Adhesion Mrkrs: CD18/11b
CHLA-DIFL
Leukocyte Adhesion Deficiency Type-1. Measures expression of CD18, CD11a, CD11b and CD15s on the surface of granulocytes.
Test code(s): Leukocyte Adhesion Deficiency Type 1
MAYO-ML
Measures CD18, CD11a, and CD11b on neutrophils. Provides % data for control and patient in interpretive report for abnormal results.
Test code(s): LAD1; #81155
MCW-CIRL
PID2 test measures CD18 and CD11b on neutrophils.
Test code(s): PID2
NJH-ADL
Qualitative analysis of CD11a,b,c and CD18 on neutrophils.
Test code(s): ADHM
NSUH
LAD panel uses CD11a, CD11b, CD11c, and CD18. Percentages are reported.
Test code(s): LAD
QUEST
Leukocyte Adhesion Deficiency (LAD) Evaluation (CD15s, CD18). Flow cytometry panel measures CD11b, CD15, and CD18 expression on neutrophils.
Test code(s): 19815

CD40 Expression

CALGARY-CLS
CD40 expression on B cells is assessed. Testing includes the CD40L (CD154) assay.
Test code(s): LINK
MAYO-ML
The cell surface expression of CD40 by B cells (CD19+) is determined and expressed as being present or absent.
Test code(s): CD40; #89009
MCW-CIRL
Measures CD40 expression on B cells and CD40L expression on activated CD4 cells.
Test code(s): HIGM
NJH-ADL
CD40 expression on CD19+ B cells. Qualitative interpretation – present/absent is reported.
Test code(s): CD40M

CD40L Expression

BCH-CFCL
Immunophenotyping of CD40 Ligand (CD154)- positive T lymphocytes, which reports: CD3+CD8-CD154+, CD3+CD8-CD69+.
Test code(s): CD40 Ligand assay
CALGARY-CLS
CD40L (CD154) expression is assessed on CD3+4+8- cells after PMA/Ionomycin activation. Testing includes assessment of CD40 on B cells.
Test code(s): LINK
CCHMC-DIL
Evaluation of CD40L Upregulation (and ICOS Upregulation).
Test code(s): CD40L / ICOS
MAYO-ML
Evaluation of CD40L Upregulation, also includes assessment of binding to receptor using soluble CD40-muIg.
Test code(s): XHIM; #82964
MCW-CIRL
Measures CD40 expression on B cells and CD40L expression on activated CD4 cells.
Test code(s): HIGM
NJH-ADL
Analyzes CD40L expression in whole blood, gating on CD3+/CD8- T cells. Reports qualitative result – absent/present.
Test code(s): CD40L

DOCK8 Protein Flow

CALGARY-CLS
Assesses DOCK8 protein expression in peripheral blood lymphocyte subsets (T, B, NK).
Test code(s): DOCK8
MONTREAL-SJH
DOCK8 detection by flow cytometry.
Test code(s): Marqueurs intracytoplasmique: Autres and indicate yourself

Flow Cytometry Screening for SCID (Related to IL2RG and IL7RA)

CALGARY-CLS
Assesses both CD132 and CD127 on peripheral blood lymphocytes:
Test code(s): CD127/CD132
CCHMC-DIL
Analyzes CD132 and CD127 expression on T cells, NK cells, and B cells.
Test code(s): CD127/CD132

FOXP3 Expression

CALGARY-CLS
Reports the % and absolute count of Tregs determined by CD3+ CD4+ lowCD127+ and CD25+.
Test code(s): TREG
CCHMC-DIL
Quantifies the percent of T regs (CD25+ CD127lo) as a percentage of the CD4+ T cells, and quantifies the percentage of T regs, which express FOXP3.
Test code(s): FoxP3
CHLA-DIFL
Regulatory T-Cell (T Reg). Percent of CD4+ CD25+ CD127dim is reported.
Test code(s):
MCW-CIRL
Measures Foxp3+, CD127, CD25 expression on CD4 cells.
Test code(s): TREG
MONTREAL-SJH
Percentages of regulatory T cells: CD4+ CD25high CD127- Foxp3+ by flow cytometry.
Test code(s): FOXp3
NJH-ADL
National Jewish Health using a 4-color panel, CD3/4/CD25/ FoxP3, gating on CD25highFoxP3+ cells. Reports percentages.
Test code(s): FOXP3
NSUH
Regulatory T-Lymphocytes uses CD4, CD25, CD127, and FoxP3. Percentages are reported.
Test code(s): FoxP3

ICOS Expression

CALGARY-CLS
Reports ICOS (CD278) expression on CD3+4+8- cells pre and post stimulation with PMA/Ionomycin.
Test code(s): ICOS
CCHMC-DIL
The CD40L Assay includes measurement of ICOS before and after activation of T Cells.
Test code(s): CD40L / ICOS
NJH-ADL
Analyzes ICOS expression in whole blood, gating on CD3+/CD8- T cells. Reports qualitative result – absent/present.
Test code(s): ICOS

LRBA Expression

CALGARY-CLS
Analyzes lipopolysaccharide Responsive Beige-like Anchor (LRBA) protein expression in CD3+ T cells. Percentage and median fluorescent intensity compared to a simultaneously run normal control is reported.
Test code(s): LRBA

MHC Class I and Class II Expression

CCHMC-DIL
Class I (HLA-A, B and C) and Class II (HLA-DR) epitope expression on peripheral blood lymphocytes and monocytes are performed by multiparameter flow cytometry.
Test code(s): MHC Class I & II
CHLA-DIFL
Bare lymphocyte syndrome - MHC Class II deficiency assay.
Test code(s): T Cell Extended
MCW-CIRL
Primary Immunodeficiency 2 panel includes CD3, CD4, CD8, TCRαβ, CD69, CD45RA, CD45RO, CD16, CD56; CD19, CD20, CD27, CD5, CD14, CD15, CD11b, CD18, CD45, HLA-DR, HLA-ABC.
Test code(s): PID2
MONTREAL-SJH
Test of HLA class I expression.
Test code(s): HLA class I
Test of HLA class II expression on B lymphocytes (CD19+) and monocytes (CD14+).
Test code(s): HLA class II

Perforin Screening for Perforin Deficiency/HLH due to PRF1 Mutations

CALGARY-CLS
Assesses perforin and granzyme B expression in NK cells and CD8+ T cells.
Test code(s): HLH
CCHMC-DIL
Analyzes perforin expression in CD8+ T cells, NK cells. Also measures and reports granzyme B expression.
Test code(s): Perforin/Granzyme B
MAYO-ML
See NKSP test under NK cell subsets. Measures perforin and Granzyme B expression in cytotoxic NK cells.
Test code(s): NKSP; #60615
MCW-CIRL
Perforin, Granzyme expression in CD56 and CD8 cells.
Test code(s): PERGRA
MONTREAL-SJH
Perforin detection by flow cytometry.
Test code(s): Perforine

SAP Screening for SAP Deficiency/XLP1

CALGARY-CLS
Measures SAP expression in T and NK cells.
Test code(s): XLP1
CCHMC-DIL
Analyzes SAP expression in T cells and NK cells.
Test code(s): SAP (XLP1)
MCW-CIRL
Assay measures SAP and XIAP expression in peripheral blood lymphocytes.
Test code(s): XLP
MONTREAL-SJH
SAP detection by flow cytometry.
Test code(s): Marqueurs intracytoplasmique: Autres and indicate yourself

WASP Screening for Wiskott-Aldrich Syndrome

CALGARY-CLS
Measures WAS in peripheral blood lymphocytes and monocytes and reports percentage and median fluorescent channel compared to a simultaneously run normal control.
Test code(s): WASP
CCHMC-DIL
Regular Panel (WASP) determines WASP expression in peripheral blood lymphocytes and reports expression as a ratio to control cells.
Test code(s): WASP
WAS Transplant Monitor Panel measures WASP expression in T cells, NK cells, B cells, monocytes, and granulocytes.
Test code(s): WASP Transplant Monitor

XIAP Screening for XIAP Deficiency/XLP2

CALGARY-CLS
Assay measures both SAP and XIAP expression in lymphocyte subsets:
Test code(s): XLP2
CCHMC-DIL
Assay measures XIAP expression in T cells, NK cells, and B cells.
Test code(s): XIAP (XLP2)
MCW-CIRL
Assay measures SAP and XIAP expression in peripheral blood lymphocytes.
Test code(s): XLP
MONTREAL-SJH
XIAP detection by flow cytometry.
Test code(s): Marqueurs intracytoplasmique: Autres and indicate yourself

ZAP-70 screening for SCID due to ZAP-70 deficiency

CALGARY-CLS
ZAP-70 protein expression is measured in T and NK cell subsets.
Test code(s): ZAP70 SCID
CCHMC-DIL
Assay measures ZAP-70 expression in T cells and NK cells.
Test code(s): ZAP-70

HIV (LIMITED FLOW CYTOMETRIC T CELL QUANTITATION)

ARUP
Lymphocyte Subset Panel 3 - T-Cell Subsets (CD4 and CD8), Absolute Counts Only. Reported; Abs CD3, CD4, CD8, and abs CD4:CD8 ratio. By Quantitative Flow Cytometry.
Test code(s): 0095853
Lymphocyte Subset Panel 1 - CD4 Absolute Count Only. Reported; abs CD4. By Quantitative Flow Cytometry.
Test code(s): 0095854
Lymphocyte Subset Panel 2 - CD4 Percent and Absolute. Reported; % CD4, and Abs CD4. By Quantitative Flow Cytometry.
Test code(s): 0095885
Lymphocyte Subset Panel 4 - T-Cell Subsets Percent and Absolute, Whole Blood, Reported; %CD3, abs CD3, %CD4, abs CD4, %CD8, abs CD8, CD4:CD8 Ratio. By Quantitative Flow Cytometry.
Test code(s): 0095950
CHLA-DIFL
T Cell Standard. The percentages and absolute numbers of lymphocytes that are CD3 (Total T cells), CD3+CD4+ (T helper cells), CD3+CD8+ (T cytotoxic cells), CD4:CD8 ratio, CD 3+ HLA-DR+ (Activated T cells) are reported.
Test code(s): T cell Standard
MAYO-ML
The percentages and absolute counts for CD3+T cells, CD4+ T cells, CD8+ T cells and CD45+ absolute lymphocyte count.
Test code(s): TCD4 (#84348)
MCW-CIRL
Absolute T4 Panel includes CD3, CD4, CD8, CD14, CD45.
Test code(s): AT4
NJH-ADL
CD3/4/8.
Test code(s): CD3SB
CD4/8.
Test code(s): CD48
QUEST
Lymphocyte Subset Panel 4. Absolute Lymphocytes, Percentage CD4, Absolute CD4, Percentage CD8, Absolute CD8, CD4/CD8 Ratio (calculated).
Test code(s): 3061N
Lymphocyte Subset Panel 3. Absolute Lymphocytes, %CD 3 (Mature T-Cells), Absolute CD3+ Cells, %CD4 (Helper Cells), Absolute CD4+ Cells, %CD8 (Suppressor T-Cells), Absolute CD8+ Cells, Helper/Suppressor Ratio.
Test code(s): 5033N
Lymphocyte Subset Panel 5. Absolute Lymphocytes, Percentage CD4, Absolute CD4.
Test code(s): 5058N

DISEASE-SPECIFIC OR PHENOTYPE SPECIFIC FUNCTIONAL TESTS (EXCLUDING T CELL AND NK CELL FUNCTION TESTS)

CD40L Expression/Upregulation (Screening for XHIGM)

BCH-CFCL
Immunophenotyping of CD40 Ligand (CD154)- positive T lymphocytes, which reports: CD3+CD8-CD154+, CD3+CD8-CD69+.
Test code(s): CD40 Ligand assay
CALGARY-CLS
CD40L (CD154) expression is assessed on CD3+4+8- cells after PMA/Ionomycin activation. Testing includes assessment of CD40 on B cells.
Test code(s): LINK
CCHMC-DIL
Evaluation of CD40L Upregulation (and ICOS Upregulation).
Test code(s): CD40L / ICOS
MAYO-ML
Evaluation of CD40L Upregulation, also includes assessment of binding to receptor using soluble CD40-muIg.
Test code(s): XHIM; #82964
MCW-CIRL
Measures CD40 expression on B cells and CD40L expression on activated CD4 cells.
Test code(s): HIGM
NJH-ADL
Analyzes CD40L expression in whole blood, gating on CD3+/CD8- T cells. Reports qualitative result – absent/present.
Test code(s): CD40L

DHR/Oxidative Burst (Screening for CGD)

ARUP
Neutrophil Oxidative Burst Assay (DHR). White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by Semi-Quantitative Flow Cytometry.
Test code(s): 0096657
BCH-CFCL
Neutrophil’s Oxidative Burst, which reports: Neutrophil Oxidative Index (MFI DHR+ / MFI DHR-), and % Responding Polymorphonuclear Cells.
Test code(s): DHR assay
CALGARY-CLS
Functional CGD screening assay, using DHR123 loaded neutrophils pre and post stimulation with PMA to induce oxidative burst reaction.
Test code(s): NFUN
CCHMC-DIL
Classic dihydrorhodamine (DHR) 123 assay.
Test code(s): Neutrophil Oxidative Burst (DHR)
CHLA-DIFL
Chronic granulomatous disease diagnostic screening assay for oxidative burst using dihydrorhodamine (DHR) 123.
Test code(s): CGD Flow
IOWA
Classic dihydrorhodamine (DHR) 123 assay with PMA stimulation.
Test code(s): SPL8
MAYO-ML
Classic dihydrorhodamine (DHR) 123 assay.
Test code(s): DHR (for both PMA and fMLP stimulants); #62764; DHRP (only for PMA); #62765; DHRF (only for fMLP); #62766.
MCW-CIRL
Classic dihydrorhodamine (DHR) 123 assay with PMA and fMLP stimulation.
Test code(s): NEUOXB
MONTREAL-SJH
Neutrophil’s oxidation (DHR) by flow cytometry.
Test code(s): Activité Oxidative des Neutrophiles
NJH-ADL
Classic dihydrorhodamine (DHR) 123 assay. Offers NBT assay as back up.
Test code(s): DHR; NBT
NSUH
Neutrophil oxidation assay uses dihydrorhodamine (DHR) 123 and PMA. Report includes MFI ratio.
Test code(s): DHR
QUEST
Neutrophil Function, Oxidative Burst. Classic dihydrorhodamine (DHR) 123 assay.
Test code(s): 37523
UCLA-UIC
Classic dihydrorhodamine (DHR) 123 assay with PMA stimulation.
Test code(s): GOB
VIRACOR
Oxidative Burst: Whole blood is stimulated with PMA or buffer alone, Formation of the reactive oxidants during the oxidative burst is monitored by the addition and enzymatic oxidation of a fluorogenic substrate, DHR 123. The level of reactive oxygen radicals is determined by flow cytometry.
Test code(s): 403002

Functional Screen for XIAP Deficiency/XLP2

MCW-CIRL
Assay tests TLR4 function by assessing the ability of monocytes to produce TNF-α in response to LPS and MDP (to test XIAP function) stimulation.
Test code(s): TLREC

GMCSF Receptor Function

CCHMC-DIL
Measurement of STAT5 phosphorylation following GMCSF stimulation. (GMCSF autoantibody testing also available.)
Test code(s): GM-CSF Receptor Stimulation
MONTREAL-SJH
Measures phosphorylation/dephosphorylation STAT5 (IL2, IL3, IL15, and GMCSF-induced) by flow cytometry.
Test code(s): Étude functionnelle and indicate yourself
NJH-ADL
ELISA measurement of GM-CSF autoantibodies, and reflex testing of P-STAT5 inhibition for positive samples.
Test code(s): GMCSFA

IL-10R Function Screen

MCW-CIRL
Assay measures the ability of IL-10 to suppress cytokine synthesis from monocytes, specifically the secretion of IL-6 in response to LPS stimulation. Detects functional defects due to IL10R deficiency. In addition, a dose response of IL10 can detect defects in STAT3 (Hyper-IgE).
Test code(s): CYTIBD

Lymphocyte Apoptosis

CCHMC-DIL
Fas-mediated apoptosis assay. T cells are treated with APO-1-3 and protein A to induce cell death, following a 11 day culture, using Concanavalin A and IL-2. The % cell loss is calculated and reported.
Test code(s): Apoptosis (Fas, mediated)

Mendelian Susceptibility to Mycobacterial Disease

MCW-CIRL
Measures pSTAT1 response to IFNγ and evaluation of IL-12Rβ1 (CD212) and IFNg receptor expression.
Test code(s): MSMD
MONTREAL-SJH
Two tests can be ordered: 1 - IL-12/gIFN cytokines secretion (ELISA). 2 - Measures of phosphorylation/dephosphorylation of STAT1 in response to gIFN by flow cytometry.
Test code(s): Étude functionnelle and indicate yourself
NJH-ADL
Phenotypic analysis of IFNγR1 and IL-12 (beta chain) receptors.
Test code(s): IFNGR, IL12R

SCID Screening (IL2RG and JAK3)

CALGARY-CLS
Functional assay in which peripheral blood T cells are stimulated with IL-6 to induce phosphorylation of STAT3 or alternatively, stimulation with IL-2 to induce phosphorylation of STAT5.
Test code(s): PSTAT3 or PSTAT5
CCHMC-DIL
Analyzes phosphorylation of STAT5 in peripheral blood lymphocytes following stimulation of peripheral blood mononuclear cells with IL-2.
Test code(s): pSTAT5
MONTREAL-SJH
Measures phosphorylation/dephosphorylation of STAT3 (IL10 and IL6-induced) and STAT5 (IL2, IL3, IL15, and GMCSF-induced) by flow cytometry.
Test code(s): Étude functionnelle and indicate yourself

TH17 Assay

CALGARY-CLS
Measures % of Th17 cells in the CD3+4+ subset following stimulation with PMA/Ionomycin.
Test code(s): TH17
CCHMC-DIL
Quantifies CD4+ CCR6+ T cells in comparison to an age-based range, and measures IL-17 production following stimulation with Phorbol 12-Myristate 13 Acetate (PMA) and Ionomycin which allows quantitation of TH17 cells.
Test code(s): Th-17 Enumeration
MCW-CIRL
Assay measures T cell-specific IFNγ and IL-17 production by PMA/Ionomycin stimulated PBMCs.
Test code(s): THIL17
MONTREAL-SJH
Measures IL-17 production following stimulation with Phorbol 12-Myristate 13 Acetate (PMA) and Ionomycin in CD4 T cells.
Test code(s): Étude functionnelle and indicate yourself
NJH-ADL
Offers % of TH1 (IFNg) and TH17 (IL-17) CD4 T cells in PBMC following PMA/Ionomycin stimulation.
Test code(s): TH17C

Toll-like Receptor Function (and Functional Screen for NEMO Deficiency)

ARUP
Toll-Like Receptor Function. Results for TNF alpha, IL-1 beta, IL-6, and CXCL10 are reported as pg/mL. Method: Cell Culture/Quantitative Multiplex Bead Assay.
Test code(s): 0051589
MCW-CIRL
Assay tests TLR4 function by assessing the ability of monocytes to produce TNF-α in response to LPS and MDP (to test XIAP function) stimulation.
Test code(s): TLREC
MONTREAL-SJH
TLR 2-4 activation: CD62L shedding assay (only local samples for technical reasons) and IL-6 and TNF secretion (ELISA).
Test code(s): TLR2-4
TLR 3 activation in NK cells (CD69 and CD25 expression) by flow cytometry.
Test code(s): TLR3
VIRACOR
Whole blood stimulation with multiple ligands, Lipoteichoic Acid (TLR2), Zymosan (TLR2/6), LPS (TLR4), Flagellin (TLR5), and Bacterial DNA (TLR9) followed by TNF-alpha analysis.
Test code(s): 403091

T CELL FUNCTION TESTS, INCLUDING RESPONSES TO MITOGENS/ANTIGENS, AND ASSESMENT OF CYTOTOXICITY

ARUP
Lymphocyte Mitogen Proliferation. 3H Thymidine incorporation assay measuring lymphocyte proliferation to the nonspecific mitogens phytohemagglutinin (PHA), concanavalin A (Con A), and pokeweed.
Test code(s): 0096043
Lymphocyte Antigen and Mitogen Proliferation Panel. 3H Thymidine incorporation assay measuring lymphocyte proliferation to phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen.
Test code(s): 0096056
Lymphocyte Antigen and Mitogen Proliferation Panel with Cytokine Response. Cell culture and multiplex bead assay, which measures lymphocyte proliferation and cytokine release following stimulation with Candida and tetanus antigens and the mitogens phytohemagglutinin, concanavalin A, and pokeweed mitogen.
Test code(s): 20131174
BCH-CFCL
T cell Mitogen-induced Lymphocyte Proliferation (Phytohemagglutinin, Concanavalin A, Anti-CD3), and T cell Antigen-induced Lymphocyte Proliferation (Candida Albicans, Tetanus Toxoid).
Test code(s): T cell Mitogen Proliferation and T cell Antigen Proliferation
CALGARY-CLS
Ability of mononuclear cells to proliferate in response to PWM, ConA, PHA and StaphA is assessed by flow cytometry cell counts and reported as a proliferation index.
Test code(s): MSA
CCHMC-DIL
3H Thymidine incorporation assay measuring lymphocyte proliferation to the antigens Candida albicans and Tetanus/Diphtheria toxoid.
Test code(s): Antigen Stimulation
3H Thymidine incorporation assay measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Concanvalin A (Con A), and Pokeweed (PWM).
Test code(s): Mitogen Stimulation
CD8+ T cell degranulation (CD107a) following exposure to anti-CD3 coated P815 cells.
Test code(s): T cell degran
CHLA-DIFL
3H Thymidine incorporation assay measuring lymphocyte proliferation to Phytohemagglutinin, Concanavalin A, pokeweed mitogens, and several antigens including CMV, HSV, VZV, ADV, Candida, CD3CD28 and tetanus antigens. Note: CD3CD28 induced proliferation by special request.
Test code(s): PWM Blastogenesis, ConA, PHA Blastogenesis, CMV Blastogenesis, HSV Blastogenesis, VZV Blastogenesis, Candida blastogenesis, ADV Blastogenesis, Tetanus Blastogenesis.
IOWA
Lymphocyte Proliferation to Mitogens. 3H Thymidine incorporation assay measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Concanvalin A (CON A), Pokeweed mitogen (PWM), Interleukin 2 (IL2) and anti-CD3.
Test code(s): SPL3
Lymphocyte Proliferation to Antigens. 3H Thymidine incorporation assay measuring lymphocyte proliferation to the antigens Tetanus/Diphtheria, Candida albicans and alloantigen.
Test code(s): SPL4
MAYO-ML
CMV-specific CD8 T cell immune competence is evaluated using MHC class I tetramers (flow assay also includes a quantitative component for CMV-specific CD8 T cells for 5 HLA alleles – A1, A2, B7, B8 and B35). Functional component includes CD107a/b expression and IFN-g production of CMV-specific CD8 T cells.
Test code(s): CMVC8; #88826
CD8 T cell degranulation (CD107a) and measurement of IFN-g after CD8 T cell activation with PMA/ionomycin.
Test code(s): GLIC; #89317
Flow based methodology measuring lymphocyte proliferation to an anti-CD3 panel (soluble anti-CD3, aCD3+aCD28, aCD3+ IL-2).
Test code(s): LPA3P; #62205
Flow based methodology measuring lymphocyte proliferation to the antigens Candida albicans and tetanus toxoid.
Test code(s): LPAG; #60592
Flow based methodology measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA) and Pokeweed (PWM).
Test code(s): LPMG; #60591
MCW-CIRL
Cytokine Production by Mononuclear Cells in Response to Antigen and Mitogen Stimulation. Antigens (Candida albicans, tetanus toxoid) and mitogens (phytohemagglutinin, concanavalin A, pokeweed) are tested independently. Peripheral Blood Mononuclear Cell (PBMC) cytokine production responses to these antigens and mitogens are determined by quantitative multiplex bead assay. The following cytokines (and receptor) are reported: IL-2, sIL-2R (sCD25), IL-4, IL-5, IL-10, IL-13, IL-1b, IL-6, IL-17, TNF-a, and IFN-g.
Test code(s): 2013111
CFSE-labeled T cells are assessed for their ability to proliferate to cytokines: anti-CD3, anti-CD3/IL-2, anti-CD3/IL-7, anti-CD3/IL-15, anti-CD3/anti-CD28.
Test code(s): TINTL
Flow-based methodology. CFSE-labeled T cells are assessed for their ability to proliferate to mitogens: ConA/IL-2, PHA/IL-2, PMA/Ionomycin and anti-CD3 with and without IL-2.
Test code(s): TMITO
MONTREAL-SJH
Degranulation CD3+CD8+ blasts (cytotoxic CD8 T lymphocytes) by flow cytometry.
Test code(s): Dégranulation des lymphocytes T
T cell proliferation after PHA and anti-CD3 (OKT3) antibody stimulation by flow cytometry.
Test code(s): PHA, OKT3
NJH-ADL
3H Thymidine incorporation in response to tetanus toxoid (3 concentrations) and Candida.
Test code(s): LCAND, LTET
3H Thymidine incorporation assay to measure lymphocyte proliferation to Phytohemagglutinin (PHA), Concanvalin A (Con A), and Pokeweed (PWM) (3 concentrations each). Available separately with
Test code(s): LPHA, LCONA, LPMW, or together:
NSUH
Lymphocyte proliferation to Antigen uses 3H thymidine incorporation method to measure lymphocyte proliferation to Tetanus toxoid and Candida albicans.
Test code(s): Antigen
Lymphocyte proliferation to Mitogen uses 3H thymidine incorporation method to measure lymphocyte proliferation to PHA, ConA, and PWM.
Test code(s): Mitogen
QUEST
Candida-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Candida.
Test code(s): 17270
Tetanus-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Tetanus Toxoid.
Test code(s): 17274
Tuberculin PPD-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Tuberculin PPD (TB PPD).
Test code(s): 17680
Mitogen-induced Lymphocyte Proliferation Panel. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Phytohemagglutinin (PHA), Concanavalin A (Con A), Pokeweed Mitogen (PWM). Test available by prior arrangement only.
Test code(s): 91976
Antigen-induced Lymphocyte Proliferation Panel (Candida, Tetanus, TB PPD). Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Candida, Tetanus Toxoid, Tuberculin PPD (TB PPD). Test available by prior arrangement only.
Test code(s): 91977
Mitogen- and Antigen-induced Lymphocyte Proliferation Panel. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Phytohemagglutinin (PHA), Concanavalin A (Con A), Pokeweed Mitogen (PWM), Candida, Tetanus Toxoid, Tuberculin PPD (TB PPD). Test available by prior arrangement only.
Test code(s): 91978
Phytohemagglutinin (PHA)-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Phytohemagglutinin (PHA). Test available by prior arrangement only.
Test code(s): 91980
Pokeweed Mitogen (PWM)-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Pokeweed Mitogen (PWM). Test available by prior arrangement only.
Test code(s): 91981
Concanavalin A (Con A)-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Concanavalin A (Con A). Test available by prior arrangement only.
Test code(s): 91982
UCLA-UIC
Flow-based methodology measuring lymphocyte proliferation to the mitogen Phytohemagglutinin (PHA) and to the antigens Candida Albicans and Tetanus Toxoid.
Test code(s): LPA-1M2A
Flow-based methodology measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Pokeweed (PWM) and Concanavalin A (Con A).
Test code(s): LPA-3M
Flow-based methodology measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Pokeweed (PWM) and Concanavalin A (Con A), and to the antigens Candida Albicans and Tetanus Toxoid.
Test code(s): LPA-3M2A
VIRACOR
T cell Function Analysis: PBMC isolation from whole blood, stimulation with various antigens or mitogens, flow cytometry analysis for CD69 upregulation and interferon gamma production.
Test code(s): 403066 and 403065
Intracellular Cytokine Panel: Whole blood is stimulated with PMA and ionomycin. Following stimulation cells are stained with antibodies to identify the cell population (CD4, CD8, NK and NKT cells) and cytokine (interferon gamma, IL4, or TNF) of interest.
Test code(s): 404474

NK CELL FUNCTIONAL TESTS

CALGARY-CLS
Evaluation of patient NK cell CD107a surface expression following exposure to K562 cells.
Test code(s): CD107A
CCHMC-DIL
Evaluation of CD107a surface expression/degranulation following exposure of NK cells to K562 cells.
Test code(s): CD107a Mobilization (NK Cell Degran)
Spontaneous NK cell cytotoxicity against K562 cells (4h stimulation, Cr51 assay).
Test code(s): NK Function
IOWA
Spontaneous NK cell cytotoxicity against K562 cells (4h stimulation, Cr51 assay).
Test code(s): SPL1
Enhanced NK cell cytotoxicity with Interleukin 2 stimulation against K562 cells (4h stimulation, Cr51 assay).
Test code(s): SPL2
MAYO-ML
Spontaneous NK cell cytotoxicity against K562 cells (4h stimulation, Cr51 assay).
Test code(s): NKCP; #28562
MCW-CIRL
Flow cytometry assay using CFSE-labeled K562 target cells and 7-AAD incorporation by apoptotic target cells following culture with PBMC’s at various Effector:Target ratios. Test includes evaluation of CD107a surface expression/degranulation following exposure of NK cells to K562 cells.
Test code(s): CYTAPO
MONTREAL-SJH
Degranulation of NK cells by flow cytometry.
Test code(s): Dégranulation de NK
NSUH
NK function test measures CD107a surface expression after exposing NK cells to K562 cells.
Test code(s): NK function
QUEST
Natural Killer Cells, Functional. PBMC isolation from whole blood, incubation with fluorescently labeled K562 at various E:T target ratios, following incubation percent lysis is determined by flow cytometry.
Test code(s): 34184
UCLA-UIC
Flow cytometry assay using CFSE-labeled K562 target cells and Sytox Red incorporation by apoptotic target cells following culture with PBMCs at various Effector:Target ratios.
Test code(s): NKCC
VIRACOR
BMC isolation from whole blood, incubation with K562 (fluorescently labeled) at various E:T target ratios, following incubation percent lysis is determined by flow cytometry.
Test code(s): 30035

NEUTROPHIL FUNCTIONAL TESTS

Bactericidal Assays and Chemotaxis

NJH-ADL
Bactericidal assay. Analysis of the ability of isolated neutrophils to clear S. aureus – manual method.
Test code(s): BACT
Neutrophil chemotaxis. Modified Boyden chamber method utilizing peripheral blood and zymosan activated serum as chemotactic agent. Readout is distance migrated in microns.
Test code(s): CTX

DHR/Oxidative Burst

ARUP
Neutrophil Oxidative Burst Assay (DHR). White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by Semi-Quantitative Flow Cytometry.
Test code(s): 0096657
BCH-CFCL
Neutrophil’s Oxidative Burst, which reports: Neutrophil Oxidative Index (MFI DHR+ / MFI DHR-), and % Responding Polymorphonuclear Cells.
Test code(s): DHR assay
CALGARY-CLS
Functional CGD screening assay, using DHR123 loaded neutrophils pre and post stimulation with PMA to induce oxidative burst reaction.
Test code(s): NFUN
Functional CGD screening assay, using DHR123 loaded neutrophils pre and post stimulation with PMA to induce oxidative burst reaction.
Test code(s): NFUN
CCHMC-DIL
Classic dihydrorhodamine (DHR) 123 assay.
Test code(s): Neutrophil Oxidative Burst (DHR)
CHLA-DIFL
Chronic granulomatous disease diagnostic screening assay for oxidative burst using dihydrorhodamine (DHR) 123.
Test code(s): CGD Flow
IOWA
Classic dihydrorhodamine (DHR) 123 assay with PMA stimulation.
Test code(s): SPL8
MAYO-ML
Classic dihydrorhodamine (DHR) 123 assay.
Test code(s): DHR (for both PMA and fMLP stimulants); #62764; DHRP (only for PMA); #62765; DHRF (only for fMLP); #62766.
MCW-CIRL
Classic dihydrorhodamine (DHR) 123 assay with PMA and fMLP stimulation.
Test code(s): NEUOXB
MONTREAL-SJH
Neutrophil’s oxidation (DHR) by flow cytometry.
Test code(s): Activité Oxidative des Neutrophiles
NJH-ADL
Classic dihydrorhodamine (DHR) 123 assay. Offers NBT assay as back up.
Test code(s): DHR; NBT
NSUH
Neutrophil oxidation assay uses dihydrorhodamine (DHR) 123 and PMA. Report includes MFI ratio.
Test code(s): DHR
QUEST
Neutrophil Function, Oxidative Burst. Classic dihydrorhodamine (DHR) 123 assay.
Test code(s): 37523
UCLA-UIC
Classic dihydrorhodamine (DHR) 123 assay with PMA stimulation.
Test code(s): GOB
VIRACOR
Oxidative Burst: Whole blood is stimulated with PMA or buffer alone, Formation of the reactive oxidants during the oxidative burst is monitored by the addition and enzymatic oxidation of a fluorogenic substrate, DHR 123. The level of reactive oxygen radicals is determined by flow cytometry.
Test code(s): 403002

CYTOKINE PANELS, INDIVIDUAL CYTOKINES AND SOLUBLE PROTEINS

Cytokine Panels

ARUP
Cytokine Panel. Reported: Interleukin 2 receptor (CD25) soluble; Interferon gamma; Interleukin-1beta, 2, 4, 5, 6, 8, 10, 12, 13, 17; TNF-alpha. Quantitative Multiplex Bead Assay.
Test code(s): 0051394
Cytokine Panel, TH1. Reported: Interleukin 2, 12; Interleukin 2 receptor (CD25) soluble; Interferon gamma. Quantitative Multiplex Bead Assay.
Test code(s): 0051408
Cytokine Panel, TH2. Reported: Interleukin 4, 5, 10, 13. Quantitative Multiplex Bead Assay.
Test code(s): 0051518
Cytokine Panel, Monokines. Reported: Interleukin 1beta, 6, 8, TNF alpha. Quantitative Multiplex Bead Assay.
Test code(s): 0051524
CCHMC-DIL
Plasma Cytokine Panel. Luminex Multiplex Panel includes IL-1beta, 2, 4, 5, 6, 8, 10; Interferon-gamma; TNF-alpha; GM-CSF.
Test code(s): Cytokines, Plasma or CSF
NJH-ADL
TH1 Cytokine 4 Plex Panel: Interleukin 2 [IL-2], Interleukin 12 [IL-12p70], Interferon Gamma [IFNg], Tumor Necrosis Factor alpha [TNFa]. Quantitative Multiplex Bead Assay.
Test code(s): PLASMA TH1
TH2 Cytokine 4 Plex Panel: Interleukin 4 [IL-4], Interleukin 5 [IL-5], Interleukin 6 [IL-6], Interleukin 10 [IL-10]. Quantitative Multiplex Bead Assay.
Test code(s): PLASMA TH2P
TH1/TH2 Panel A: Interleukin 2 [IL-2], Interleukin 4 [IL-4], Interleukin 5 [IL-5], Interleukin 6 [IL-6], Interleukin 10 [IL-10], Interleukin 12 [IL-12p70], Interferon Gamma [IFNg], Tumor Necrosis Factor alpha [TNFa]. Quantitative Multiplex Bead Assay.
Test code(s): PLASMA THPNLA
TH1/TH2 Panel B: Interleukin 2 [IL-2], Interleukin 4 [IL-4], Interleukin 5 [IL-5], Interleukin 10 [IL-10], Interferon Gamma [IFNg], Tumor Necrosis Factor alpha [TNFa]. Quantitative Multiplex Bead Assay.
Test code(s): PLASMA THPNLB
VIRACOR
Th1/Th2 Panel Includes: Interferon-gamma, Interleukin-13, Interleukin-12p70, Interleukin-10, Interleukin-5, Interleukin-4, and Interleukin-2. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 403025 Serum or 403026 Plasma
Pro-inflammatory Panel Includes: Interleukin-1b, Interleukin-6, Interleukin-8, and TNF-alpha. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 403027 Serum or 403028 Plasma

Individual Cytokines and Soluble Proteins

CCHMC-DIL
B-cell Activating Factor.
Test code(s): BAFF

ARUP
Interferon gamma. Multiplex Bead Assay.
Test code(s): 0051531
VIRACOR
Interferon gamma. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1271 Serum or 1221 Plasma

ARUP
Interleukin 10. Multiplex Bead Assay.
Test code(s): 0051534
VIRACOR
Interleukin-10. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1221 Serum or 1222 Plasma

ARUP
Interleukin 12. Multiplex Bead Assay.
Test code(s): 0051530
VIRACOR
Interleukin-12p70. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1213 Serum or 1223 Plasma

ARUP
Interleukin 13. Multiplex Bead Assay.
Test code(s): 0051535
VIRACOR
Interleukin-13. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1214 Serum or 1224 Plasma

ARUP
Interleukin 17. Multiplex Bead Assay.
Test code(s): 2013115
VIRACOR
Interleukin-17. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 40034 Serum or 40033 Plasma

CCHMC-DIL
Interleukin-18.
Test code(s): Interleukin–18 (IL-18)

ARUP
Interleukin 1beta. Multiplex Bead Assay.
Test code(s): 0051536
QUEST
Interleukin 1 beta. Measured by ELISA.
Test code(s): 1757
VIRACOR
Interleukin 1b. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1215 Serum or 1225 Plasma

ARUP
Interleukin 2. Multiplex Bead Assay.
Test code(s): 0051588
QUEST
Quest Diagnostics: Interleukin 2. Measured by ELISA.
Test code(s): 34161
VIRACOR
Interleukin-2. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 30041 Serum or 1226 Plasma

ARUP
Interleukin 4. Multiplex Bead Assay.
Test code(s): 0051532
VIRACOR
Interleukin-4. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1217 Serum or 1227 Plasma

ARUP
Interleukin 5. Multiplex Bead Assay.
Test code(s): 0051533
VIRACOR
Interleukin-5. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 30042 Serum or 1228 Plasma

ARUP
Interleukin 6. Multiplex Bead Assay.
Test code(s): 0051537
QUEST
Interleukin 6. Measured by high sensitivity ELISA.
Test code(s): 34473X
VIRACOR
Interleukin-6. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1257 Serum or 1258 Plasma

ARUP
Interleukin 8. Multiplex Bead Assay.
Test code(s): 0051538
VIRACOR
Interleukin-8. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1219 Serum or 1229 Plasma

CCHMC-DIL
Neopterin.
Test code(s): Neopterin, Plasma or CSF

CCHMC-DIL
Soluble CD163.
Test code(s): Soluble CD163

CCHMC-DIL
Soluble Fas Ligand.
Test code(s): Soluble Fas-Ligand (sFasL)
MONTREAL-SJH
ALPS- Plasma FAS Ligand/CD95 (ELISA).
Test code(s): Dosage du FAS LIGAND

ARUP
Interleukin 2 Receptor (CD25), Soluble. By Quantitative Multiplex Bead Assay.
Test code(s): 0051529
CCHMC-DIL
2 Receptor (CD25), Soluble.
Test code(s): Soluble IL-2R (Soluble CD25)
QUEST
Interleukin-2 Receptor EIA. Soluble IL2R levels measured by EIA.
Test code(s): 34298
VIRACOR
sIl-2R. The assay employs a sandwich immunoassay format.
Test code(s): 30057

ARUP
Tumor Necrosis Factor – alpha. Multiplex Bead Assay.
Test code(s): 0051539
VIRACOR
TNF-alpha. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.
Test code(s): 1220 Serum or 1230 Plasma

COMPLEMENT TESTS (INCLUDING INDIVIDUAL COMPONENTS, FUNCTIONAL ASSAYS, AND SPLIT PRODUCTS) - GROUPED BY SPECIALTY LAB

MAYO-ML
Complement, Alternative Pathway (AH50), Functional Serum (separately orderable).
Test code(s): AH50; #88676
Atypical Hemolytic Uremic Syndrome (aHUS) Complement Panel.
Test code(s): AHUSD; #64881
C1 Esterase (C1ES) Inhibitor Antigen, Serum (nephelometry).
Test code(s): C1ES; #8198
Complement C1q, Serum (nephelometry).
Test code(s): C1Q; #8851
C1q Complement Functional, Serum (Automated Liposome Lysis [ALL]).
Test code(s): C1QFX; #83374
C2 Complement Functional, Serum (ALL).
Test code(s): C2; #81835
C2 Complement Antigen, Serum (RID – reflex testing only).
Test code(s): C2AG; #84141
C2 Complement Functional (no reflex, ALL).
Test code(s): C2FXN; #32137
Complement C3, Serum (nephelometry).
Test code(s): C3; #8174
C3 Functional, Serum (ALL).
Test code(s): C3FX; #81090
Complement C4, Serum (nephelometry).
Test code(s): C4; #88171
C4d Complement Activation Fragment, Plasma (AHUSD only).
Test code(s): C4D; #62584
C4 Functional, Serum (ALL).
Test code(s): C4FX; #83391
C5 Complement, Antigen, Serum (nephelometry).
Test code(s): C5AG; #9266
C5 Functional, Serum (ALL).
Test code(s): C5FX; #83392
C6 Functional, Serum (ALL
Test code(s): C6FX; #83393
C7 Functional, Serum (ALL).
Test code(s): C7FX; #81064
C8 Functional, Serum (ALL).
Test code(s): C8FX; #81065
C9 Functional, Serum (ALL).
Test code(s): C9FX; #81066
CBB Complement Activation Fragment, Plasma (AHUSD only).
Test code(s): CBB; #62585
Complement, Total, Serum.
Test code(s): COM; #8167
Factor B Complement Antigen (AHUSD only, nephelometry).
Test code(s): FBCA; #62588
C1 Esterase Inhibitor Functional Assay, Serum (ELISA).
Test code(s): FC1EQ; #81493
Factor H Complement Antigen (AHUSD only, nephelometry).
Test code(s): FHCA; #62587
SC5b-9 Complement Activation Complex, Plasma (AHUSD only).
Test code(s): SC5B9; # 62586
VIRACOR
C1 Esterase Inhibitor Functional.
Test code(s): 1112
C1 Esterase Inhibitor Protein.
Test code(s): 1113
C1q Complement.
Test code(s): 1115
Mannan Binding Lectin (MBL).
Test code(s): 33
Mannan-Binding Lectin Pathway Function Test (C4b).
Test code(s): 34
C4 Complement.
Test code(s): 8

Autoantibodies to Individual Components

ARUP
Immune Complex Panel (by Flow and ELISA).
Test code(s): 0050667
NJH-ADL
Autoantibody to C1q (C1q-CLR, by ELISA).
Test code(s): C1QAB
C3 Nephritic factor (by Immunofixation Electrophoresis).
Test code(s): C3NEF
Autoantibody to C1-inhibitor by (ELISA).
Test code(s): CEIAP
Autoantibody to Factor H (by ELISA).
Test code(s): FHAB

Component Levels

ARUP
C1-Esterase Inhibitor Panel (by ELISA, Turb and Neph).
Test code(s): 0050139
C1-Esterase Inhibitory (by Quant Neph).
Test code(s): 0050140
Complement Component 2 (by RID).
Test code(s): 0050148
Complement Components 3 and 4 (by Quant. Immunoturbid).
Test code(s): 0050149
Complement Component 3 (by Quant. Immunoturbidimetry).
Test code(s): 0050150
Complement Component 4 (by Quant. Immunoturbidimetry).
Test code(s): 0050155
Complement Component 5 (by RID).
Test code(s): 0050156
Complement Factor B (by RID).
Test code(s): 0051720
Complement Component 1Q Level (by RID).
Test code(s): 0099130
NJH-ADL
C1q level (by RID).
Test code(s): C1Q
C1r level (by RID).
Test code(s): C1RL
C1s level (by RID).
Test code(s): C1SL
C2 level (by RID).
Test code(s): C2L
C3 level (by nephelometry).
Test code(s): C3
C4 level (by nephelometry).
Test code(s): C4
C5 level (by RID).
Test code(s): C5L
C6 level (by RID).
Test code(s): C6L
C7 level (by RID).
Test code(s): C7L
C8 level (by RID).
Test code(s): C8L
C9 level (by RID).
Test code(s): C9L
C1-esterase inhibitor level (C1-INH, by RID).
Test code(s): CEIQ
Circulating immune complexes (C1q-binding and C3d).
Test code(s): CIC
Factor B level (by RID).
Test code(s): FBL
Factor D level (by ELISA).
Test code(s): FDL
Factor H level (by RID).
Test code(s): FH
Factor I level (by RID).
Test code(s): FIL
Mannose binding lectin (by ELISA).
Test code(s): MLEC
Properdin level (by ELISA).
Test code(s): PROP
QUEST
Mannose binding lectin (by ELISA).
Test code(s): 16039X
C1-esterase inhibitor protein (quantitative, by EIA).
Test code(s): 298
Properdin Factor B (by nephelometry).
Test code(s): 300
C7 level (by RID).
Test code(s): 34888X
C8 level (by RID).
Test code(s): 34889X
C9 level (by RID).
Test code(s): 34896
C3 level (by nephelometry).
Test code(s): 351
C4 level (by nephelometry).
Test code(s): 353
C5 level (by RID).
Test code(s): 354
Circulating immune complexes (C1q-binding, by ELISA).
Test code(s): 36735
C2 level (by RID).
Test code(s): 433X
C6 level (by RID).
Test code(s): 464
C1q level (by RID).
Test code(s): 981X

Functional Assays

ARUP
C1-Esterase Inhibitor Functional (by ELISA).
Test code(s): 0050141
Complement Activity Enzyme Immunoassay, Total (by ELISA).
Test code(s): 0050198
C1q Binding Assay (by ELISA).
Test code(s): 0050301
Complement Activity, Alternative Pathway (AH50, by RID).
Test code(s): 2005373
NJH-ADL
C1 function (by hemolytic assay).
Test code(s): C1F
C1q function (by hemolytic assay).
Test code(s): C1QH
C2 function (by hemolytic assay).
Test code(s): C2F
C3 function (by hemolytic assay).
Test code(s): C3F
C4 function (by hemolytic assay).
Test code(s): C4F
Rapid screen for deficiency of late components (C5, C6, C7, C8, C9, CH50).
Test code(s): C59S
C5 function (by hemolytic assay).
Test code(s): C5F
C6 function (by hemolytic assay).
Test code(s): C6F
C7 function (by hemolytic assay).
Test code(s): C7F
C8 function (by hemolytic assay).
Test code(s): C8F
C9 function (by hemolytic assay).
Test code(s): C9F
C1 esterase inhibitor function, Chromogenic.
Test code(s): CEICHR
Factor D function (by hemolytic assay).
Test code(s): FDF
Factor B function (by hemolytic assay).
Test code(s): PFBF
QUEST
C1-esterase inhibitor protein (functional, by EIA).
Test code(s): 297

Individual Complement Split Products

NJH-ADL
Bb level (by ELISA).
Test code(s): BBLL
C3a desArg level (by RIA).
Test code(s): C3AL
C4a desArg level (by RIA).
Test code(s): C4AL
C4d level (by ELISA).
Test code(s): C4D
Ratio of C4d to C4.
Test code(s): C4RAT
C5a desArg level (by RIA).
Test code(s): C5AL
iC3b level (by ELISA).
Test code(s): IC3B
SC5b-9 level (by ELISA).
Test code(s): SC5B9
QUEST
C3a desarg (by ELISA).
Test code(s): 17689X
C3d fragment (by EIA).
Test code(s): 4943N

OTHER SPECIALIZED TESTS

Autoantibodies to Cytokines: Detection and Functional Assays

CCHMC-DIL
Measurement of STAT5 phosphorylation following GMCSF stimulation.
Test code(s): GM-CSF Receptor Stimulation
Measurement of autoantibodies to GMCSF by ELISA.
Test code(s): GMCSF Autoantibody
MONTREAL-SJH
Measures of phosphorylation/dephosphorylation STAT5 (IL2, IL3, IL15, and GMCSF-induced) by flow cytometry.
Test code(s): Étude functionnelle and indicate yourself
NJH-ADL
ELISA measurement of GM-CSF autoantibodies, and reflex testing of P-STAT5 inhibition for positive samples.
Test code(s): GMCSFA

MONTREAL-SJH
Measures of phosphorylation of STAT1 in response to gIFN by flow cytometry.
Test code(s): Étude functionnelle and indicate yourself
NJH-ADL
Binding and functional analysis of autoantibodies to IFNg by ELISA and reflex P-STAT1 inhibition assay.
Test code(s): IFNGAB

Molecular Tests (non-Gene Sequencing)

MAYO-ML
Both visual interpretation of spectratype and diversity ratio information included in report.
Test code(s): TCRVB; #62930
QUEST
T-cell Receptor (TCR) Gamma Gene Rearrangement, PCR. Interrogates the T-cell receptor gamma locus (TCRG) by a PCR method based on the BIOMED-2 consensus.
Test code(s): 90509
T-cell Clonality Panel (TCRG, TCRB), PCR. Interrogates both the T-cell receptor beta (TCRB) locus and the T-cell receptor gamma (TCRG) locus by a PCR method based on the BIOMED-2 consensus.
Test code(s): 91445
T-cell Receptor (TCR) Beta Gene Rearrangement, PCR. Interrogates the T-cell receptor beta locus (TCRB) by a PCR method based on the BIOMED-2 consensus.
Test code(s): 91446

MAYO-ML
TREC quantitatoin reported as copies per million CD3+ T cells.
Test code(s): TREC; #87959

Specialized Serologic Testing for Vaccine Response Evaluation (not including routine vaccine titers)

VIRACOR
Functional Antibody Analysis- Pneumococcal Avidity. A modification of the standard pneumococcal assay, using varying concentrations of ammonium thiocyanate. Bound antibodies are detected with an antibody that recognizes human IgG. The IgG antibody concentration is calculated using a standard curve referenced to WHO standard serum 89SF. The avidity is calculated based on binding with various concentrations of thiocyanate. Multiple Panels available with varying number of serotypes:
Test code(s): 401734, 401846, 401839, and 4017345

MCW-CIRL
ELISA assay evaluates Salmonella typhi polysaccharide vaccine-specific IgG antibodies, which can be done in (suspected) immunodeficient patients on immunoglobulin replacement therapy.
Test code(s): STIGG

Therapeutic Monitoring Lymphocyte Depletion

CCHMC-DIL
Flow based assay to measure alemtuzumab levels in whole blood.
Test code(s): Alemtuzumab Level

CCHMC-DIL
Quantifies CD52 on T cells, NK cells, B cells, monocytes.
Test code(s): CD52 Expression
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