DLI Lab Directory Search

GENERAL IMMUNOPHENOTYPING (FLOW CYTOMETRY)

B Cell Enumeration and/or Extended Phenotyping

CHCO- Immunology Lab

Comprehensive B cell phenotyping: Reports percentages of the following CD19+ B cells: naïve (CD27-IgD+), total non-class switched memory (CD27+IgD+), class-switched memory (CD27+IgD-), CD21low, transitional (IgM+CD38+), plasmablasts (IgM-CD38++), IgM only CD27+ B cells, IgM+IgD+ CD27+ B cells, IgM-IgD- class-switched CD27+ B cells

Test code(s): LAB9066

B Cell Phenotyping

BCH-CFCL

B cell Number. Reports percentage and absolute number of B cells. Memory B cells. Reports percentages of B cells that are switched memory (CD27+ and IgD-), unswitched memory (CD27+ and IgD+), and naive (CD27- IgD+). CVID Panel. Reports CD19+ CD21low CD38low B cells, Transitional B cells (CD24hi CD38high), Plasmablasts (CD24low CD38high), and Marginal zone like B cells (CD24high CD38 low).

Test code(s):

CALGARY-CLS

Immunodeficiency screen panel includes percentages and absolute numbers of T cells, B cells, NK cells, TNK (CD3+16/56+), CD3+4+, CD3+8+, DNT (CD3+4-8-), CD4/CD8 ratio, Naïve B cells (CD19+IgD+CD27-), Memory non-class switched B cells (CD19+IgD+CD27+), Memory class switched B cells (CD19+IgD-CD27+), Naïve Helper T cells (CD3+4+CD45RA+CD27+), Terminally Differentiated Helper T cell (CD3+4+45RA+27-), Central Memory Helper T cell (CD3+4+45RA-27+), Effector Memory Helper T cell (CD3+4+45RA-27-), Naïve Cytotoxic T cells (CD3+8+CD45RA+CD27+), Terminally Differentiated Cytotoxic T cell cell (CD3+8+45RA+27-), Central Memory Cytotoxic T cell (CD3+8+45RA-27+), Effector Memory Cytotoxic T cell (CD3+8+45RA-27-). CD45RA/CD45RO also assessed.

Test code(s): Test code(s): IDEF

CCHMC-DIL

B Cell Panel: Reports several Immature, Transitional, Naïve, Memory, and Class-Switched Memory B cell populations, and also Plasma-Blast populations.

Test code(s):

CD4+ Recent Thymic Emigrants

ARUP

CD4+ T-Cell Recent Thymic Emigrants (RTEs). Reports percentage and absolute numbers of CD4+CD31+CD45RA+ (RTEs). By Quantitative Flow Cytometry.

Test code(s): 2010179

BCH-CFCL

Immunophenotyping of Recent Thymic Emigrants, which reports: CD4+CD31+CD45RA+Recent Thymic Emigrants.

Test code(s): RTE Panel

CALGARY-CLS

Reports the % and absolute count of CD3+CD4+CD45RA+CD31+ recent thymic emigrants.

Test code(s): RTE

CCHMC-DIL

CD45RA/RO panel includes evaluation of CD31 expression as a marker of recent thymic emigrants.

Test code(s): CD45RA/RO

CHCO- Immunology Lab

Naïve, memory and recent thymic emigrant T cell panel: Reports percentage of naïve (CD45RA+), memory (CD45RO+), recent thymic emigrants (CD4+CD31+), effector memory (CD45RA-CCR7-), central memory (CD45RA-CCR7+), TEMRA (CD45RA+CCR7-) CD4 and CD8 T cells, and CD4+CD31+ recent thymic emigrants

Test code(s): LAB849

CHLA-DIFL

The percentages and absolute numbers of lymphocytes that are newly formed CD4+ cells with following phenotypes of CD4+CD45+RA+ cells: CD3+4+/RA+RO+, CD3+4+/RA+/CD31+ and CD3+4+/RA+62L+ are reported.

Test code(s): T Cell Subsets (Memory and Naïve)

MAYO-ML

Reports the percentage of CD4+ 45RA+ CD31+ T cells.

Test code(s): CD4RT; #89504

MONTREAL-SJH

Naïve/memory T lymphocyte subsets: CD4+CD45RO+, CD4+CD45RA+, CD4+CD45RA+ CD31+, and CD8+ CD45RA+ CCR7+ (naïve), CD8+CD45RA-CCR7+ (CM), CD8+CD45RA-CCR7- (TEM), CD8+CD45RA+CCR7- (TEMRA).

Test code(s): CD45RA/CD4, CD45RA-31/CD4, CD45RO/CD4, CD45RA/CD8, CD45RA-31/CD8, CD45RO/CD8

NJH-ADL

Identifies RTEs using a 4-color panel: CD3/CD4/CD45RA/CD31. Reports percentages.

Test code(s): CD4RTE

Lymphocyte Subset Enumeration

ARUP

Natural Killer Cells Enumeration: The results reported are the percentage of lymphocytes that are Natural killer (NK) cells and the absolute count of NK-cells per µL of blood. Only those cells that express CD45 and either CD16 or CD56 (or both) but not CD3 or CD19 are reported. By Quantitative Flow Cytometry.

Test code(s): 0092404

Lymphocyte Subset Panel 3: T-Cell Subsets (CD4 and CD8), Absolute Counts Only. By Quantitative Flow Cytometry. Reported; Abs CD3, CD4, CD8, and abs CD4:CD8 ratio.

Test code(s): 0095853

Lymphocyte Subset Panel 1: CD4 Absolute Count Only. Reported; abs CD4. By Quantitative Flow Cytometry.

Test code(s): 0095854

Lymphocyte Subset Panel 2: CD4 Percent and Absolute. Reported; % CD4, and Abs CD4. By Quantitative Flow Cytometry.

Test code(s): 0095885

Lymphocyte Subset Panel 5: Total Lymphocyte Enumeration. Reported; % CD3, abs CD3, % CD4, abs CD4, % CD8, abs CD8, CD4: CD8 ratio, % CD19, abs CD19, % NK-cells, abs NK-cells. By Quantitative Flow Cytometry.

Test code(s): 0095892

Isolated Panel for T cells (Monitoring post ATG): Lymphocyte Transplantation CD3. Reported: WBC (K/µL), % Lymph, Absolute Lymph Count, %CD3, abs CD3. By Quantitative Flow Cytometry.

Test code(s): 0095949

Lymphocyte Subset Panel 4: T-Cell Subsets Percent and Absolute. Reported; %CD3, abs CD3, %CD4, abs CD4, %CD8, abs CD8, CD4:CD8 Ratio. By Quantitative Flow Cytometry.

Test code(s): 0095950

BCH-CFCL

Analysis of lymphocytes populations, which reports: The percentages and absolute numbers of lymphocytes that are CD3+ (Total T cells), CD3+CD4+ (T helper cells), CD3+CD8+ (T cytotoxic cells), CD4:CD8 ratio, CD3-CD19+ (B cells), CD-CD16/56+ (NK cells).

Test code(s): Lymphocyte Subsets or T/B/NK Panel

CALGARY-CLS

Test code(s): IDEF

CCHMC-DIL

The percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+) are reported.

Test code(s): Lymphocyte Subsets

CHCO- Immunology Lab

TBNK: Reports percentages and absolute counts of CD3+, CD4+, CD8+, CD19+ and CD16/56+ lymphocytes (CBC required).

Test code(s): LAB7755

T Cell Subsets: Reports percentage and absolute counts of CD3+, CD4+ and CD8+ T cells (CBC required)

Test code(s): LAB7756

CD3: Reports percentage and absolute counts of CD3+ T cells (CBC required)

Test code(s): LAB7758

CHLA-DIFL

T cell Extended panel: The percentages and absolute numbers of lymphocytes that are CD3+ (Total T cells), CD3+CD4+ (T helper cells), CD3+CD8+ (T cytotoxic cells), CD4:CD8 ratio, CD3-CD19+ (B cells), CD-CD16/56+ (Natural Killer (NK) cells), and CD3+ HLA-DR+ (Activated T cells) are reported.

Test code(s): T-Cell Extended

T cell Standard panel: The percentages and absolute numbers of lymphocytes that are CD3+ (Total T cells), CD3+CD4+ (T helper cells), CD3+CD8+ (T cytotoxic cells), CD4:CD8 ratio, and CD3+ HLA-DR+ (Activated T cells) are reported.

Test code(s): T-Cell Standard

MAYO-ML

Test code(s): TBBS; #9336

MCW-CIRL

AT4 Panel: CD3, CD4, CD8, CD14, CD45 To monitor patient's individual CD4 and CD8 T cell populations. Panel is routinely used in determining therapeutic considerations for HIV+ patients. Reports percentages and absolute numbers of lymphocytes that are T cells (CD3+ Total T Cells), T-helper (CD3+, CD4+) and T-cytotoxic (CD3+, CD8+).

Test code(s): AT4

PID1 Panel: CD3, CD4, CD8, CD16, CD56, CD19, CD14, CD45. Reports percentages and absolute numbers of lymphocytes that are T cells (CD3+ Total T Cells), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD56+), (CD56+, CD3-), (CD56+, CD3+), and (CD56+, CD16+), and B lymphocytes (CD19+). A CD4:CD8 ratio is also reported.

Test code(s): PID1

PPID2 Panel: CD3, CD4, CD8, CD45RA, CD45RO, CD16, CD56, CD19, CD14, CD45, HLA-DR. Reports percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD56+), (CD56+, CD3-), (CD56+, CD3+), and (CD56+, CD16+), and B lymphocytes (CD19+). T Cell subset percentages reported are activated T cells (HLA-DR+CD4+) and (HLA-DR+CD8+), T cell memory (CD4+CD45RA+) and (CD4+CD45RO+). A CD4:CD8 ratio is also reported.

Test code(s): PID2

MONTREAL-SJH

Lymphocyte subset phenotyping by quantitative flow cytometry: B lymphocyte subsets CD19+, CD19+CD27+, CD19+CD20+.

Test code(s): CD19, CD27/CD19, CD20

Lymphocyte subset phenotyping by quantitative flow cytometry: NK cells: CD3-CD56+.

Test code(s): CD3 (-) CD56 (+)

Lymphocyte subset phenotyping by quantitative flow cytometry: T lymphocytes: CD3+, CD3+CD4+, CD3+CD8.

Test code(s): CD3, CD4, CD8

NCH

Lymphocyte Subset (T, B, NK Cells) Quantitation in Bronchoalveolar Lavage – This is a flow cytometric lymphocyte subset quantitation (CD45+ ALC, CD14 for monocyte exclusion, CD3+, CD4+ and CD8+ T cells, CD19/20+ B cells, CD16/56+ NK cells, CD4:CD8 ratio, CD3+CD4-CD8- DNT cells. This test is useful for the evaluation of patients with primary immunodeficiencies (inborn errors of immunity), patients undergoing solid-organ transplantation (during induction and post-transplant monitoring), patients undergoing hematopoietic cell transplantation (HCT) for assessment of immune reconstitution and competence, patients receiving immunomodulatory therapies, and any clinical context where a more detailed lymphocyte subset evaluation is needed (outside a CBC + differential). Results are typically reported within 24-48h from receipt of sample in the laboratory.

Test code(s): TBNKBL

NJH-ADL

CD3/4/8: The percentages and absolute numbers are reported.

Test code(s): CD3SB

CD4/8: The percentages and absolute numbers are reported.

Test code(s): CD48

Individual markers - CD4: The percentage and absolute number are reported.

Test code(s): CD4M

Individual markers - CD16/56: The percentage and absolute number are reported.

Test code(s): NKMK

Lymphocyte Phenotyping Panel: CD3+, CD3+/CD4+, CD3+/CD8+, CD16/56 and CD19+. CD4/CD8 ratio reported. The percentages and absolute numbers are reported.

Test code(s): TBCDC

CD3/4/8/19: The percentages and absolute numbers are reported.

Test code(s): TBSBS

NSUH

Lymphocyte subset uses CD3, CD4, CD8, CD19, CD16, and CD56. Report includes percentages and absolute counts. CD4 to CD8 ratio is also reported.

Test code(s): TnB

UCLA-UIC

Both the T-cell memory and the B-cell panels include a basic panel for lymphocyte enumeration, which reports the percentages and absolute numbers of T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), CD4:CD8 ratio, natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+), followed by an advanced panel to identify (i) T-memory: naïve (CD45RA+/CD45RO-) and Memory (CD45RA-/CD45RO+) CD4+ and CD8+ T cells; (ii) B-cell: Memory (CD27+), IgM Memory (CD27+IgM+IgD-), Unswitched Memory (CD27+IgM+IgD+), Switched Memory (CD19+CD27+IgD-IgM-) and Immature (CD21low) CD19+ B cells.

Test code(s): T-MEM or B-CELL

Lymphocyte/T cell Activation

CALGARY-CLS

Panel 1: CD3+4+ T cells are assessed for surface expression of CD154, CD69, HLA-DR, CD143, CD25, CD71, and CD96 pre and post PMA/Ionomycin stimulation. Panel 2: CD8+ T cells are assessed for expression of CD45RO and HLA-DR primarily for use in investigation of Omenn’s Syndrome or HLH. Antigen density of CD5 on CD3+8+ cells is also reported for HLH investigations.

Test code(s): LAM

CCHMC-DIL

Measures CD4+ T cell surface expression of CD69, CD25, CD154, CD134, CD95, CD71, and HLA-DR.

Test code(s): Lymphocyte Activation Markers

CHCO- Immunology Lab

Lymphocyte and T cell proliferation to PHA. Flow cytometry based assay. Reports percentage of proliferating CD45+ lymphocytes and CD3+ T cells in response to 3 concentrations of PHA

Test code(s): LAB9101

CHLA-DIFL

T Cell Activation Panel. The following populations are reported in percentages: CD25+CD3+/CD3, DR+CD3+/CD3, CD69+CD3+/CD3, CD134+CD3+/CD3, CD25+CD4+/CD3, DR+CD4+/CD3, CD69+CD4+/CD3, CD134+CD4+/CD3.

Test code(s): T Cell Subsets Activated T

MAYO-ML

The T cell phenotyping assay includes evaluation of activated (CD4+25+ and MHC class II-positive) CD4+ and CD8+ T cells.

Test code(s): TCP; #89319

MCW-CIRL

T cell activation panel: Includes CD4, CD8, CD69, and CD25 at 4 hours and 4 days after activation to measure early and late activation events:

Test code(s): TCACT

MONTREAL-SJH

Sainte Justine Hospital: Percentages of activated T cells for HLA class II DR expression on CD3+, CD3+CD4+, and CD3+CD8+ by flow cytometry.

Test code(s): HLA class II/CD3, HLA class II/CD4, HLA class II/CD8

Monocyte and Dendritic Cell Phenotyping

MAYO-ML

Dendritic Cell and Monocyte Enumeration. This test enumerates plasmacytoid dendritic cells (pDC), myeloid dendritic cells (mDC), and classical monocytes. It can be used as part of the diagnostic assessment of patients suspected of defects in innate immunity, particularly those in monocyte and dendritic cell development, which can manifest in isolation or as part of a broader clinical phenotype.

Test code(s): DCME

NCH

Monocyte Subset Quantitation – Monocytes reflect the monocyte/macrophage or mononuclear phagocytes in circulation. The majority of CD14high cells found in human blood are classified as classical monocytes while a very minor population (in healthy controls) of cells express low CD14 and high CD16 and are described as non-classical monocytes. The population between these two are referred to as the transitional subset. It is useful to quantitate and evaluate monocyte subsets in the context of certain primary immunodeficiencies (inborn errors of immunity) where such cell populations are clinically informative. Monocyte subsets can also be skewed in the context of severe infection, HIV-1 infection, therapy with steroids and other immunomodulatory agents, and are critical regulators of inflammation. There is also some evidence that the absolute count of transitional monocytes can predict cardiovascular events. Monocyte subsets in blood varies substantially during life from the neonatal period into adulthood.

Test code(s): MOSQN

NK Cell Phenotyping

ARUP

Natural Killer Cells Enumeration. The results reported are the percentage of lymphocytes that are Natural killer (NK) cells and the absolute count of NK-cells per µL of blood. Only those cells that express CD45 and either CD16 or CD56 (or both) but not CD3 or CD19 are reported. By Quantitative Flow Cytometry.

Test code(s): 0092404

Natural Killer Cell and Natural Killer T-Cell Panel. The panel reports frequencies and absolute cell counts of NK and NKT-cells, also including T and non-T cells: CD3-CD16-/+CD56bright/dim (total NK-cells), CD3-CD16+CD56dim (cytotoxic NK-cells), CD3-CD16-CD56bright (cytokine secreting NK-cells), CD3-CD57+ (CD57+NK-cells), CD3+CD56+ (CD56+ NKT-cells), CD3+CD57+ (CD57+ NKT-cells), CD45+CD3+ (T-cells) and CD45+CD3- (Non T-cells).

Test code(s): 2013805

BCH-CFCL

Immunophenotyping of Natural Killer cells which reports: percentages of CD56 high NK cells, CD56+CD16+ NK cells, and CD56-CD16 + NK cells.

Test code(s): NK Cell Phenotype

MCW-CIRL

Pergra Panel includes: CD3, CD4, CD8, CD56, CD16, CD19, CD14, CD45, Perforin, Granzyme B Perforin and Granzyme B expression in CD56+/CD16+ and CD56+/CD16- cells.

Test code(s): PERGRA

NCH

NK Cell Subset Quantitation – This test is for quantitation of the 2 major NK cell subsets in blood - cytotoxic NK cells and cytokine-producing NK cells. Useful for evaluation of patients with primary immunodeficiencies (inborn errors of immunity), in the context of hematopoietic cell transplantation and any clinical context where the distribution of these two NK cell subsets in blood is required. This test does not assess for NK cell receptors.

Test code(s): NKSQN

NKT cell Subsets (including invariant NKT cells)

CALGARY-CLS

The percentage and absolute count of CD3+ TCRvalpha24+ TCRvbeta11+ iNKT cells is reported.

Test code(s): INKT

CCHMC-DIL

Quantification of TCRValpa24 and TCR Vbeta 11 expression on invariant NKT cells.

Test code(s): iNKT

MONTREAL-SJH

Quantification of MAIT (Va 24 Vb 11) and iNKT (Va 24 Vb 11) cells.

Test code(s): NKT

Regulatory T Cell Analysis and/or FOXP3 Expression

ARUP

Regulatory T-Cell Panel. Reported: Percent and absolute numbers of CD4+ CD25+ CD127Low (Tregs), and CD45RA and CD45RO also included in the panel. By Quantitative Flow Cytometry.

Test code(s): 2010172

BCH-CFCL

Immunophenotyping of Regulatory T cells, which reports: CD4+CD25+CD127+ T cells, CD4+CD25highCD127low T cells.

Test code(s): Treg Panel

CALGARY-CLS

Reports the % and absolute count of Tregs determined by CD3+ CD4+ lowCD127+ and CD25+.

Test code(s): TREG

CCHMC-DIL

Quantifies the percent of T regs (CD25+ CD127 lo) as a percentage of the CD4+ T cells, and quantifies the percentage of T regs, which express FOXP3.

Test code(s): FoxP3

CHCO- Immunology Lab

Regulatory T cells: Reports percentage of CD3+CD4+CD25+FOXP3+ T cells and mean fluorescence intensity of FoxP3

Test code(s): LAB9111

CHLA-DIFL

Regulatory T-Cell (T Reg). Percent of CD4+ CD25+ CD127dim is reported.

Test code(s):

MAYO-ML

Identifies percentages and absolute numbers of CD4+CD25+CD127loCD45RO+ (Nat Tregs) and CD4+CD25+CD127loCD45RA+ Naive Tregs.

Test code(s): TREGS; #89318

MONTREAL-SJH

Percentages of regulatory T cells: CD4+ CD25high CD127- Foxp3+ by flow cytometry.

Test code(s): FOXp3

NJH-ADL

National Jewish Health using a 4-color panel, CD3/4/CD25/ FoxP3, gating on CD25highFoxP3+ cells. Reports percentages.

Test code(s): FOXP3

NSUH

Regulatory T-Lymphocytes uses CD4, CD25, CD127, and FoxP3. Percentages are reported.

Test code(s): FoxP3

T Cell Phenotyping (Naïve T Cells, Memory T Cells, Recent Thymic Emigrants/Extended Phenotyping)

ARUP

Lymphocyte Subset Panel 6: Total Lymphocyte Enumeration with CD45RA and CD45RO. Reported; %CD3, abs CD3, % CD4, abs CD4, % CD45RA, abs CD45RA, % CD45RO, abs CD45RO, % CD8, abs CD8, CD4:CD8 Ratio, % CD19, abs CD19, % NK-cells, abs NK-cells. By Quantitative Flow Cytometry.

Test code(s): 0095862

Lymphocyte Subset Panel 7: Congenital Immunodeficiencies. Reported; %CD2, abs CD2, % CD3, Abs CD3, %HLA-DR, abs HLA-DR, % CD4, abs CD4, % CD45RA, Abs CD45RA, % CD8, abs CD8, CD4: CD8 ratio, % CD19, abs CD19, % NK-cells, abs NK-cells. By Quantitative Flow Cytometry.

Test code(s): 0095899

BCH-CFCL

Immunophenotyping of Naïve and Memory T cells, which reports: TEMRA CD4+CD45RA+CCR7-T cells, Naïve CD4+CD45RA+CCR7+T cells, Effector memory CD4+CD45RA-CCR7-T cells, Central memory CD4+CD45RA-CCR7+T cells, TEMRA CD8+CD45RA+CCR7-T cells, Naïve CD8+CD45RA+CCR7+T cells, Effector memory CD8+CD45RA-CCR7-T cells, Central memory CD8+CD45RA-CCR7+T cells.

Test code(s): T Cell Memory Panel

CALGARY-CLS

Immunodeficiency screen panel includes percentages and absolute numbers of T cells, B cells, NK cells, TNK (CD3+16/56+), CD3+4+, CD3+8+, DNT (CD3+4-8-), CD4/CD8 ratio, Naïve B cells (CD19+IgD+CD27-), Memory non-class switched B cells (CD19+IgD+CD27+), Memory class switched B cells (CD19+IgD-CD27+), Naïve Helper T cells (CD3+4+CD45RA+CD27+), Terminally Differentiated Helper T cell (CD3+4+45RA+27-), Central Memory Helper T cell (CD3+4+45RA-27+), Effector Memory Helper T cell (CD3+4+45RA-27-), Naïve Cytotoxic T cells (CD3+8+CD45RA+CD27+), Terminally Differentiated Cytotoxic T cell (CD3+8+45RA+27-), Central Memory Cytotoxic T cell (CD3+8+45RA-27+), Effector Memory Cytotoxic T cell (CD3+8+45RA-27-). CD45RA/CD45RO also assessed.

Test code(s): IDEF

CCHMC-DIL

CD45RA/RO Assay. Reports percentages of naïve, central memory, effector memtory, and TEMRA CD4+ and CD8+ populations using a combination of markers including CD45RA, CD45RO, CCR7, and CD27. Also reports CD31 expression as a marker of recent thymic emigrants.

Test code(s):

CHCO- Immunology Lab

Test code(s): LAB849

T follicular helper cells (Tfh): Reports percentage of CD3+CD4+CXCR5+ T cells

Test code(s): LAB9410

CHLA-DIFL

Naïve T Cell Subsets. The percentages and absolute numbers of lymphocytes that are newly formed CD4+ cells with following phenotypes of CD4+CD45+RA+ cells: CD3+4+/RA+RO+, CD3+4+/RA+/CD31+ and CD3+4+/RA+62L+ are reported.

Test code(s): T cell Subsets (Memory and Naïve)

MAYO-ML

Quantitates naïve (global and CD62L+), memory (global, central, and effector memory), and activated (CD4+25+ and MHC class II-positive) CD4+ and CD8+ T cells using antibodies directed against CD3, CD4, CD8, CD45RO, CD45RA, HLADR, CD27, CD62L, CD25, and CD28.

Test code(s): TCP; #89319

MCW-CIRL

CVID Panel:CD3, CD4, CD8, CD16, CD56, CD19, CD21, CD27, IgD, HLA-DR, CD45RA, CD45RO, CD14, CD45. Reports percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD56+), (CD56+, CD3-), (CD56+, CD3+), and (CD56+, CD16+), and B lymphocytes (CD19+). T Cell subset percentages reported are activated T cells (HLA-DR+CD4+) and (HLA-DR+CD8+), T cell memory (CD4+CD45RA+) and (CD4+CD45RO+). A CD4:CD8 ratio is also reported. Reports percentage and absolute number of CD19+ as well as percentages of B cell subsets.

Test code(s): CVID

PID2 Panel: CD3, CD4, CD8, CD45RA, CD45RO, CD16, CD56, CD19, CD14, CD45, HLA-DR. Reports percentages and absolute numbers of lymphocytes that are T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), natural killer (CD56+), (CD56+, CD3-), (CD56+, CD3+), and (CD56+, CD16+), and B lymphocytes (CD19+). T Cell subset percentages reported are activated T cells (HLA-DR+CD4+) and (HLA-DR+CD8+), T cell memory (CD4+CD45RA+) and (CD4+CD45RO+). A CD4:CD8 ratio is also reported

Test code(s): PID2

MONTREAL-SJH

Naïve/memory T lymphocytes: CD4+CD45RO+, CD4+CD45RA+, CD4+CD45RA+CD31+/ CD8+CD45RA+CCR7+ (naïve), CD8+CD45RA-CCR7+ (CM), CD8+CD45RA-CCR7- (TEM), CD8+CD45RA+CCR7- (TEMRA).

Test code(s): CD45RA/CD4, CD45RA-31/CD4, CD45RO/CD4, CD45RA/CD8, CD45RA-31/CD8, CD45RO/CD8

NJH-ADL

National Jewish Health runs a memory and naïve T cell panel that includes CD3/4/CD45RA/CD45RO.

Test code(s): C4RARO

NSUH

Naïve/memory T-Lymphocyte subset uses CD3, CD4, CD8, CD45RA, and CD45RO. Percentages are reported.

Test code(s): CD45RARO

UCLA-UIC

The T-cell memory panel includes a basic panel for lymphocyte enumeration, which reports the percentages and absolute numbers of T cells (CD3+), T-helper (CD3+, CD4+), T-cytotoxic (CD3+, CD8+), CD4:CD8 ratio, natural killer (CD16+CD56+, CD3-), and B lymphocytes (CD19+), followed by an advanced panel to identify naïve (CD45RA+/CD45RO-) and Memory (CD45RA-/CD45RO+) CD4+ and CD8+ T cells.

BCH-CFCL

Immunophenotyping of γδ and CD4-CD8- αβ T cells, which reports: CD3+CD4-CD8-TCR alpha/beta T cells, CD3+TCR gamma/delta T cells. See also ALPS panel.

Test code(s): ALPS Panel

CALGARY-CLS

Reports the % and absolute count of TCRα/β and TCRγ/δ positive T cells:

Test code(s): TCRABGD

CCHMC-DIL

Quantifies the percentages of T cells expression α/β TCR and γ/δ TCR.

Test code(s): TCR α/β TCR γ/δ

CHCO- Immunology Lab

TCRαβ and TCRγδ subsets: Reports percentage of TCRαβ and TCRγδ T cells

Test code(s): LAB7755

CHLA-DIFL

Quantifies the percentages of T cells expression α/β TCR and γ/δ TCR.

Test code(s): TCR α/β TCR γ/δ

MONTREAL-SJH

Percentages of TCR α/β /CD3 and TCR γ/δ /CD3.

Test code(s): TCR α/β TCR γ/δ /CD3

NJH-ADL

Quantifies the percentages of TCRα/β and γ/δ T cells.

Test code(s): TCABGD

NSUH

T-lymphocyte TCR subset uses CD3, CD4, CD8, TCRα/β, and TCRγ/δ. Percentages are reported.

Test code(s): TCR

DISEASE-SPECIFIC FLOW CYTOMETRY TESTS OR PANELS

ALPS Panel

Test code(s): CVID; #87993

NJH-ADL

Reports BAFF-R qualitatively as present/absent and includes commentary comparing the expression level to that of a date matched normal control.

Test code(s): BAFFR

BTK Protein Flow

ARUP

Bruton Tyrosine Kinase (BTK) Protein Expression by Flow Cytometry (Qualitative Flow Cytometry).

Test code(s): 2012002

CALGARY-CLS

Bruton Tyrosine Kinase (BTK) Protein Expression by Flow Cytometry in B cells and monocytes.

Test code(s): BTK

MAYO-ML

Analyzes BTK expression in B cells and monocytes.

Test code(s): BTK; #89011

MCW-CIRL

BTK Panel includes: CD3, CD4, CD8, CD19, BTK, CD16, CD56, CD14, CD45 Evaluates BTK expression in B cells and monocytes.

Test code(s): BTK

CD18, CD11a/CD11b, and CD15s Expression (Screening for LAD)

ARUP

Leukocyte Adhesion Deficiency Panel. Panel measures CD11b, CD15, and CD18 on neutrophils. By Semi-Quantitative Flow Cytometry.

Test code(s): 2004359

BCH-CFCL

Leukocyte Adhesion Deficiency, which reports: CD18+ cells, CD15s+ cells.

Test code(s): LAD Panel

CALGARY-CLS

Measures expression of CD18, CD11a, CD11b, CD11c (Type I LAD) and CD15s (Type II LAD) on the surface of granulocytes.

Test code(s): LAD

CCHMC-DIL

Measures CD18 and CD11b expression on neutrophils.

Test code(s): Neutrophil Adhesion Mrkrs: CD18/11b

CHCO- Immunology Lab

Leukocyte Adhesion Deficiency 1. Qualitative report (normal/abnormal) of CD18 and CD11b expression on neutrophils and monocytes

Test code(s): LAB9077

CHLA-DIFL

Leukocyte Adhesion Deficiency Type-1. Measures expression of CD18, CD11a, CD11b and CD15s on the surface of granulocytes.

Test code(s): Leukocyte Adhesion Deficiency Type 1

MAYO-ML

Measures CD18, CD11a, and CD11b on neutrophils. Provides % data for control and patient in interpretive report for abnormal results.

Test code(s): LAD1; #81155

MCW-CIRL

NPF Panel includes: CD45, CD15, CD18 CD18 and CD15 expression is evaluated on granulocytes.

Test code(s): NPF (formerly NEUOXB)

NJH-ADL

Qualitative analysis of CD11a,b,c and CD18 on neutrophils.

Test code(s): ADHM

NSUH

LAD panel uses CD11a, CD11b, CD11c, and CD18. Percentages are reported.

Test code(s): LAD

QUEST

Leukocyte Adhesion Deficiency (LAD) Evaluation (CD15s, CD18). Flow cytometry panel measures CD11b, CD15, and CD18 expression on neutrophils.

Test code(s): 19815

CD40 Expression

CALGARY-CLS

CD40 expression on B cells is assessed. Testing includes the CD40L (CD154) assay.

Test code(s): LINK

MAYO-ML

The cell surface expression of CD40 by B cells (CD19+) is determined and expressed as being present or absent.

Test code(s): CD40; #89009

MCW-CIRL

HIGM Panel Includes: CD3, CD4, CD8, CD154, CD40-Fc, CD19, CD40, CD16, CD56, CD14, CD45 Measures CD40 expression on B cells and CD40L expression on activated CD4 cells. Measures binding of CD40L on activated CD4 cells to recombinant CD40 (CD40-muIg).

Test code(s): HIGM

NJH-ADL

CD40 expression on CD19+ B cells. Qualitative interpretation – present/absent is reported.

Test code(s): CD40M

CD40L Expression

BCH-CFCL

Immunophenotyping of CD40 Ligand (CD154)- positive T lymphocytes, which reports: CD3+CD8-CD154+, CD3+CD8-CD69+.

Test code(s): CD40 Ligand assay

CALGARY-CLS

CD40L (CD154) expression is assessed on CD3+4+8- cells after PMA/Ionomycin activation. Testing includes assessment of CD40 on B cells.

Test code(s): LINK

CCHMC-DIL

Evaluation of CD40L Upregulation (and ICOS Upregulation). Also assesses CD40L binding to CD40-Ig Fusion Protein.

Test code(s): CD40L / ICOS

MAYO-ML

Evaluation of CD40L Upregulation, also includes assessment of binding to receptor using soluble CD40-muIg.

Test code(s): XHIM; #82964

MCW-CIRL

Test code(s): HIGM

NJH-ADL

Analyzes CD40L expression in whole blood, gating on CD3+/CD8- T cells. Reports qualitative result – absent/present.

Test code(s): CD40L

DOCK8 Protein Flow

CALGARY-CLS

Assesses DOCK8 protein expression in peripheral blood lymphocyte subsets (T, B, NK).

Test code(s): DOCK8

CHCO- Immunology Lab

DOCK8 expression: Reports expression of DOCK8 in CD3+T cells, CD19+B cell and CD14+monocytes qualitatively. Mean fluorescence intensity of DOCK8 expression is reported

Test code(s): LAB935

MAYO-ML

The test detects the expression of dedicator of cytokinesis 8 (DOCK8) in T cells, B cells, NK cells, and monocytes in the peripheral blood. It can be used as a screening step prior to genetic testing for DOCK8; to confirm the finding of an established pathogenic alteration in DOCK8 at the protein level; to examine a reported variant of undetermined significance (VUS); and to evaluate the potential presence of somatic reversion in a patient with DOCK8 deficiency. It can help distinguish DOCK8 deficiency from conditions with overlapping clinical manifestations, including Job syndrome (AD-HIES), ZNF341 deficiency, and severe atopic dermatitis.

Test code(s): DOCK 8

MONTREAL-SJH

DOCK8 detection by flow cytometry.

Test code(s): Marqueurs intracytoplasmique: Autres and indicate yourself

Flow Cytometry Screening for SCID (Related to IL2RG and IL7RA)

CALGARY-CLS

Assesses both CD132 and CD127 on peripheral blood lymphocytes:

Test code(s): CD127/CD132

FOXP3 Expression

CALGARY-CLS

Reports the % and absolute count of Tregs determined by CD3+ CD4+ lowCD127+ and CD25+.

Test code(s): TREG

CCHMC-DIL

Quantifies the percent of T regs (CD25+ CD127lo) as a percentage of the CD4+ T cells, and quantifies the percentage of T regs, which express FOXP3.

Test code(s): FoxP3

CHCO- Immunology Lab

Regulatory T cells: Reports percentage of CD3+CD4+CD25+FOXP3+ T cells and mean fluorescence intensity of FoxP3

Test code(s): LAB9111

CHLA-DIFL

Regulatory T-Cell (T Reg). Percent of CD4+ CD25+ CD127dim is reported.

Test code(s):

MCW-CIRL

Treg panel includes: CD3, CD4, CD8, CD45RA, CD45RO, CD19, CD16, CD56, CD14, CD45, HLA-DR, CD25, FoxP3. Evaluates lymphocyte subpopulations and the presence of Foxp3+ T regulatory cells.

Test code(s): TREG

Measures Foxp3+, CD127, CD25 expression on CD4 cells.

Test code(s): TREG

MONTREAL-SJH

Percentages of regulatory T cells: CD4+ CD25high CD127- Foxp3+ by flow cytometry.

Test code(s): FOXp3

NJH-ADL

National Jewish Health using a 4-color panel, CD3/4/CD25/ FoxP3, gating on CD25highFoxP3+ cells. Reports percentages.

Test code(s): FOXP3

NSUH

Regulatory T-Lymphocytes uses CD4, CD25, CD127, and FoxP3. Percentages are reported.

Test code(s): FoxP3

ICOS Expression

CALGARY-CLS

Reports ICOS (CD278) expression on CD3+4+8- cells pre and post stimulation with PMA/Ionomycin.

Test code(s): ICOS

CCHMC-DIL

The CD40L Assay includes measurement of ICOS before and after activation of T Cells.

Test code(s): CD40L / ICOS

NJH-ADL

Analyzes ICOS expression in whole blood, gating on CD3+/CD8- T cells. Reports qualitative result – absent/present.

Test code(s): ICOS

LRBA Expression

CALGARY-CLS

Analyzes lipopolysaccharide Responsive Beige-like Anchor (LRBA) protein expression in CD3+ T cells. Percentage and median fluorescent intensity compared to a simultaneously run normal control is reported.

Test code(s): LRBA

MAYO-ML

The test determines the percentage and intensity of expression of lipopolysaccharide-responsive beige-like anchor (LRBA) protein on T cells and B cells in the peripheral blood. It can be used as a screening step prior to genetic testing for LRBA; to confirm the finding of an established pathogenic alteration in LRBA at the protein level; and to examine the effect of reported genetic variants of undetermined significance (VUS) on LRBA protein expression. It can help distinguish LRBA deficiency from conditions with overlapping clinical manifestations, including immune dysregulation and autoimmunity, such as immune dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX)-like syndromes; early onset hypogammaglobulinemia; common variable immune deficiency (CVID); inflammatory bowel disease (IBD); and autoimmune lymphoproliferative syndrome (ALPS).

Test code(s): LRBA

MHC Class I and Class II Expression

CCHMC-DIL

Class I (HLA-A, B and C) and Class II (HLA-DR) epitope expression on peripheral blood lymphocytes and monocytes are performed by multiparameter flow cytometry.

Test code(s): MHC Class I & II

CHLA-DIFL

Bare lymphocyte syndrome - MHC Class II deficiency assay.

Test code(s): T Cell Extended

MCW-CIRL

Test code(s): CVID

Test code(s): PID2

MONTREAL-SJH

Test of HLA class I expression.

Test code(s): HLA class I

Test of HLA class II expression on B lymphocytes (CD19+) and monocytes (CD14+).

Test code(s): HLA class II

Perforin Screening for Perforin Deficiency/HLH due to PRF1 Mutations

CALGARY-CLS

Assesses perforin and granzyme B expression in NK cells and CD8+ T cells.

Test code(s): HLH

CCHMC-DIL

Analyzes perforin expression in CD8+ T cells, NK cells. Also measures and reports granzyme B expression.

Test code(s): Perforin/Granzyme B

CHCO- Immunology Lab

Perforin expression: Reports mean fluorescence intensity of perforin expression in NK cells and CD8+ T cells

Test code(s): LAB9360

MCW-CIRL

Pergra Panel includes: CD3, CD4, CD8, CD56, CD16, CD19, CD14, CD45, Perforin, Granzyme B Perforin and Granzyme B expression in CD56+/CD16+ and CD56+/CD16- cells.

Test code(s): PERGRA

MONTREAL-SJH

Perforin detection by flow cytometry.

Test code(s): Perforine

SAP Screening for SAP Deficiency/XLP1

CALGARY-CLS

Measures SAP expression in T and NK cells.

Test code(s): XLP1

CCHMC-DIL

Analyzes SAP expression in T cells and NK cells.

Test code(s): SAP (XLP1)

MCW-CIRL

XLP Panel Includes: CD3, CD4, CD8, CD45RA, CD45RO, CD19, CD16, CD56, CD14, CD45, HLA-DR, SAP, XIAP Determines the presence of SAP and XIAP expression in lymphocytes to evaluate XLP type 1 and 2.

Test code(s): XLP

MONTREAL-SJH

SAP detection by flow cytometry.

Test code(s): Marqueurs intracytoplasmique: Autres and indicate yourself

WASP Screening for Wiskott-Aldrich Syndrome

CALGARY-CLS

Measures WAS in peripheral blood lymphocytes and monocytes and reports percentage and median fluorescent channel compared to a simultaneously run normal control.

Test code(s): WASP

CCHMC-DIL

Regular Panel (WASP) determines WASP expression in peripheral blood lymphocytes and reports expression as a ratio to control cells.

Test code(s): WASP

WAS Transplant Monitor Panel measures WASP expression in T cells, NK cells, B cells, monocytes, and granulocytes.

Test code(s): WASP Transplant Monitor

XIAP Screening for XIAP Deficiency/XLP2

CALGARY-CLS

Assay measures both SAP and XIAP expression in lymphocyte subsets:

Test code(s): XLP2

CCHMC-DIL

Assay measures XIAP expression in T cells, NK cells, B cells, Monocytes, and Granulocytes.

Test code(s): XIAP (XLP2)

MCW-CIRL

Panel Includes: CD3, CD4, CD8, CD45RA, CD45RO, CD19, CD16, CD56, CD14, CD45, HLA-DR, SAP, XIAP Determines the presence of SAP and XIAP expression in lymphocytes to evaluate XLP type 1 and 2.

Test code(s): XLP

MONTREAL-SJH

XIAP detection by flow cytometry.

Test code(s): Marqueurs intracytoplasmique: Autres and indicate yourself

ZAP-70 screening for SCID due to ZAP-70 deficiency

CALGARY-CLS

ZAP-70 protein expression is measured in T and NK cell subsets.

Test code(s): ZAP70 SCID

CCHMC-DIL

Assay measures ZAP-70 expression in T cells and NK cells.

Test code(s): ZAP-70

HIV (LIMITED FLOW CYTOMETRIC T CELL QUANTITATION)

ARUP

Lymphocyte Subset Panel 3 - T-Cell Subsets (CD4 and CD8), Absolute Counts Only. Reported; Abs CD3, CD4, CD8, and abs CD4:CD8 ratio. By Quantitative Flow Cytometry.

Test code(s): 0095853

Lymphocyte Subset Panel 1 - CD4 Absolute Count Only. Reported; abs CD4. By Quantitative Flow Cytometry.

Test code(s): 0095854

Lymphocyte Subset Panel 2 - CD4 Percent and Absolute. Reported; % CD4, and Abs CD4. By Quantitative Flow Cytometry.

Test code(s): 0095885

Lymphocyte Subset Panel 4 - T-Cell Subsets Percent and Absolute, Whole Blood, Reported; %CD3, abs CD3, %CD4, abs CD4, %CD8, abs CD8, CD4:CD8 Ratio. By Quantitative Flow Cytometry.

Test code(s): 0095950

CHLA-DIFL

T Cell Standard. The percentages and absolute numbers of lymphocytes that are CD3 (Total T cells), CD3+CD4+ (T helper cells), CD3+CD8+ (T cytotoxic cells), CD4:CD8 ratio, CD 3+ HLA-DR+ (Activated T cells) are reported.

Test code(s): T cell Standard

MAYO-ML

The percentages and absolute counts for CD3+T cells, CD4+ T cells, CD8+ T cells and CD45+ absolute lymphocyte count.

Test code(s): TCD4 (#84348)

MCW-CIRL

Absolute T4 Panel includes: CD3, CD4, CD8, CD14, CD45 To monitor patient's individual CD4 and CD8 T cell populations. Panel is routinely used in determining therapeutic considerations for HIV+ patients. Reports percentages and absolute numbers of lymphocytes that are T cells (CD3+ Total T Cells), T-helper (CD3+, CD4+) and T-cytotoxic (CD3+, CD8+).

Test code(s): AT4

NJH-ADL

CD3/4/8.

Test code(s): CD3SB

CD4/8.

Test code(s): CD48

QUEST

Lymphocyte Subset Panel 4. Absolute Lymphocytes, Percentage CD4, Absolute CD4, Percentage CD8, Absolute CD8, CD4/CD8 Ratio (calculated).

Test code(s): 3061N

Lymphocyte Subset Panel 3. Absolute Lymphocytes, %CD 3 (Mature T-Cells), Absolute CD3+ Cells, %CD4 (Helper Cells), Absolute CD4+ Cells, %CD8 (Suppressor T-Cells), Absolute CD8+ Cells, Helper/Suppressor Ratio.

Test code(s): 5033N

Lymphocyte Subset Panel 5. Absolute Lymphocytes, Percentage CD4, Absolute CD4.

Test code(s): 5058N

DISEASE-SPECIFIC OR PHENOTYPE SPECIFIC FUNCTIONAL TESTS (EXCLUDING T CELL AND NK CELL FUNCTION TESTS)

CD40L Expression/Upregulation (Screening for XHIGM)

BCH-CFCL

Immunophenotyping of CD40 Ligand (CD154)- positive T lymphocytes, which reports: CD3+CD8-CD154+, CD3+CD8-CD69+.

Test code(s): CD40 Ligand assay

CALGARY-CLS

CD40L (CD154) expression is assessed on CD3+4+8- cells after PMA/Ionomycin activation. Testing includes assessment of CD40 on B cells.

Test code(s): LINK

MAYO-ML

Evaluation of CD40L Upregulation, also includes assessment of binding to receptor using soluble CD40-muIg.

Test code(s): XHIM; #82964

MCW-CIRL

Test code(s): HIGM

NJH-ADL

Analyzes CD40L expression in whole blood, gating on CD3+/CD8- T cells. Reports qualitative result – absent/present.

Test code(s): CD40L

DHR/Oxidative Burst (Screening for CGD)

ARUP

Neutrophil Oxidative Burst Assay (DHR). White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by Semi-Quantitative Flow Cytometry.

Test code(s): 0096657

BCH-CFCL

Neutrophil’s Oxidative Burst, which reports: Neutrophil Oxidative Index (MFI DHR+ / MFI DHR-), and % Responding Polymorphonuclear Cells.

Test code(s): DHR assay

CALGARY-CLS

Functional CGD screening assay, using DHR123 loaded neutrophils pre and post stimulation with PMA to induce oxidative burst reaction.

Test code(s): NFUN

CCHMC-DIL

Classic dihydrorhodamine (DHR) 123 assay.

Test code(s): Neutrophil Oxidative Burst (DHR)

CHCO- Immunology Lab

Dihydrorhodamine 123 Neutrophil oxidative burst (DHR). Reports stimulation index

Test code(s): LAB7757

CHLA-DIFL

Chronic granulomatous disease diagnostic screening assay for oxidative burst using dihydrorhodamine (DHR) 123.

Test code(s): CGD Flow

IOWA

Classic dihydrorhodamine (DHR) 123 assay with PMA stimulation.

Test code(s): SPL8

MAYO-ML

Classic dihydrorhodamine (DHR) 123 assay.

Test code(s): DHR (for both PMA and fMLP stimulants); #62764; DHRP (only for PMA); #62765; DHRF (only for fMLP); #62766.

MCW-CIRL

NPF Panel Includes: CD45, CD15, CD18 Dihydrorhodamine (DHR) 123 assay. Measures the ability of neutrophils to generate an oxidative burst following stimulation with PMA or fMLP. The expression of CD15 and CD18 on neutrophils is also measured. Screening test for chronic granulomatous disease (CGD) and leucocyte adhesion deficiency type I and type II.

Test code(s): NPF (Formerly NEUOXB)

NPF Panel: CD45, CD15, CD18 Dihydrorhodamine (DHR) 123 assay. Measures the ability of neutrophils to generate an oxidative burst following stimulation with PMA or fMLP. The expression of CD15 and CD18 on neutrophils is also measured. Screening test for chronic granulomatous disease (CGD) and leucocyte adhesion deficiency type I and type II.

Test code(s): NPT (formerly NEUOXB)

MONTREAL-SJH

Neutrophil’s oxidation (DHR) by flow cytometry.

Test code(s): Activité Oxidative des Neutrophiles

NJH-ADL

Classic dihydrorhodamine (DHR) 123 assay. Offers NBT assay as back up.

Test code(s): DHR; NBT

NSUH

Neutrophil oxidation assay uses dihydrorhodamine (DHR) 123 and PMA. Report includes MFI ratio.

Test code(s): DHR

QUEST

Neutrophil Function, Oxidative Burst. Classic dihydrorhodamine (DHR) 123 assay.

Test code(s): 37523

UCLA-UIC

Classic dihydrorhodamine (DHR) 123 assay with PMA stimulation.

Test code(s): GOB

VIRACOR

Oxidative Burst: Whole blood is stimulated with PMA or buffer alone, Formation of the reactive oxidants during the oxidative burst is monitored by the addition and enzymatic oxidation of a fluorogenic substrate, DHR 123. The level of reactive oxygen radicals is determined by flow cytometry.

Test code(s): 403002

DNA Double-strand Break Repair Assessment in Lymphocytes

NCH

DNA Double-strand Break Repair Assessment in Lymphocytes – This test is useful for the diagnosis of monogenic defects associated with defects in DNA repair and associated radiosensitivity (XCIND - X-ray irradiation susceptibility, Cancer predisposition, Immunodeficiency and Neurological Defects), bone marrow failure syndromes, short telomere syndromes, excessive toxicity to chemotherapy and radiation. This test can be used to develop appropriate conditioning regimen selection in patients with these defects who require hematopoietic cell transplantation. DDRFL primarily assesses for the presence of DNA double-strand breaks in each lymphocyte subset (T, B and NK cells) and the associated repair mechanism (NHEJ, non-homologous end-joining), and excessive cell death following irradiation of lymphocytes. Markers used in this assay include CD45, CD3, CD19, CD335 (NKp46), pATM, pSMC1 and gamma H2AX. The test measures viability in the sample at baseline (Day 0), 1h and 24h radiosensitivity, and 24h assessment of cell death and apoptosis after irradiation.

Test code(s): DDRFL

Functional Screen for XIAP Deficiency/XLP2

MCW-CIRL

Assay tests TLR4 function by assessing the ability of monocytes to produce TNF-α in response to LPS and MDP (to test XIAP function) stimulation. Defective response can be seen in IRAK4/MyD88 deficiency and XIAP deficiency.

Test code(s): TLREC/XIAP

GMCSF Receptor Function

CCHMC-DIL

Measurement of STAT5 phosphorylation following GMCSF stimulation. (GMCSF autoantibody testing also available.)

Test code(s): GM-CSF Receptor Stimulation

MONTREAL-SJH

Measures phosphorylation/dephosphorylation STAT5 (IL2, IL3, IL15, and GMCSF-induced) by flow cytometry.

Test code(s): Étude functionnelle and indicate yourself

NJH-ADL

ELISA measurement of GM-CSF autoantibodies, and reflex testing of P-STAT5 inhibition for positive samples.

Test code(s): GMCSFA

IL-10R Function Screen

MCW-CIRL

Assay measures the ability of IL-10 to suppress IL-6 synthesis from monocytes in response to LPS stimulation, which is dependent on the functional integrity of the IL10 receptors and STAT3. Detects functional defects due to IL10R deficiency or autosomal dominant mutations in STAT3 leading to the Hyper-IgE Syndrome.

Quantifies CD4+ CCR6+ T cells in comparison to an age-based range, and measures IL-17 production following stimulation with Phorbol 12-Myristate 13 Acetate (PMA) and Ionomycin which allows quantitation of TH17 cells.

Test code(s): Th-17 Enumeration

MCW-CIRL

THIL17 Panel Includes: CD3, CD4, CD8, CD19, CD16, CD56, CD14, CD45, IFN-γ, IL-17 Assay measures T cell-specific IFN-γ and IL-17 production by PMA/Ionomycin stimulated PBMCs. Deficient expression is associated with hyper IgE syndrome (HIES)/Job’s Syndrome and also severe eczema.

Test code(s): THIL17

MONTREAL-SJH

Measures IL-17 production following stimulation with Phorbol 12-Myristate 13 Acetate (PMA) and Ionomycin in CD4 T cells.

Test code(s): Étude functionnelle and indicate yourself

NJH-ADL

Offers % of TH1 (IFNg) and TH17 (IL-17) CD4 T cells in PBMC following PMA/Ionomycin stimulation.

Test code(s): TH17C

Toll-like Receptor Function (and Functional Screen for NEMO Deficiency)

ARUP

Toll-Like Receptor Function. Results for TNF alpha, IL-1 beta, IL-6, and CXCL10 are reported as pg/mL. Method: Cell Culture/Quantitative Multiplex Bead Assay.

Test code(s): 0051589

MCW-CIRL

Assay tests TLR4 function by assessing the ability of monocytes to produce TNF-α in response to LPS and MDP (to test XIAP function) stimulation.

Test code(s): TLREC

Test code(s): TLREC/XIAP

MONTREAL-SJH

TLR 2-4 activation: CD62L shedding assay (only local samples for technical reasons) and IL-6 and TNF secretion (ELISA).

Test code(s): TLR2-4

TLR 3 activation in NK cells (CD69 and CD25 expression) by flow cytometry.

Test code(s): TLR3

VIRACOR

Whole blood stimulation with multiple ligands, Lipoteichoic Acid (TLR2), Zymosan (TLR2/6), LPS (TLR4), Flagellin (TLR5), and Bacterial DNA (TLR9) followed by TNF-alpha analysis.

Test code(s): 403091

T CELL FUNCTION TESTS, INCLUDING RESPONSES TO MITOGENS/ANTIGENS, AND ASSESMENT OF CYTOTOXICITY

ARUP

Lymphocyte Mitogen Proliferation. 3H Thymidine incorporation assay measuring lymphocyte proliferation to the nonspecific mitogens phytohemagglutinin (PHA), concanavalin A (Con A), and pokeweed.

Test code(s): 0096043

Lymphocyte Antigen and Mitogen Proliferation Panel. 3H Thymidine incorporation assay measuring lymphocyte proliferation to phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen.

Test code(s): 0096056

Lymphocyte Antigen and Mitogen Proliferation Panel with Cytokine Response. Cell culture and multiplex bead assay, which measures lymphocyte proliferation and cytokine release following stimulation with Candida and tetanus antigens and the mitogens phytohemagglutinin, concanavalin A, and pokeweed mitogen.

Test code(s): 20131174

BCH-CFCL

T cell Mitogen-induced Lymphocyte Proliferation (Phytohemagglutinin, Concanavalin A, Anti-CD3), and T cell Antigen-induced Lymphocyte Proliferation (Candida Albicans, Tetanus Toxoid).

Test code(s): T cell Mitogen Proliferation and T cell Antigen Proliferation

CALGARY-CLS

Ability of mononuclear cells to proliferate in response to PWM, ConA, PHA and StaphA is assessed by flow cytometry cell counts and reported as a proliferation index.

Test code(s): MSA

CCHMC-DIL

3H Thymidine incorporation assay measuring lymphocyte proliferation to the antigens Candida albicans and Tetanus/Diphtheria toxoid.

Test code(s): Antigen Stimulation

3H Thymidine incorporation assay measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Concanvalin A (Con A), and Pokeweed (PWM).

Test code(s): Mitogen Stimulation

CD8+ T cell degranulation (CD107a) following exposure to anti-CD3 coated P815 cells.

Test code(s): T cell degran

CHCO- Immunology Lab

Lymphocyte and T cell proliferation to PHA. Flow cytometry based assay. Reports percentage of proliferating CD45+ lymphocytes and CD3+ T cells in response to 3 concentrations of PHA

Test code(s): LAB9101

CHLA-DIFL

3H Thymidine incorporation assay measuring lymphocyte proliferation to Phytohemagglutinin, Concanavalin A, pokeweed mitogens, and several antigens including CMV, HSV, VZV, ADV, Candida, CD3CD28 and tetanus antigens. Note: CD3CD28 induced proliferation by special request.

Test code(s): PWM Blastogenesis, ConA, PHA Blastogenesis, CMV Blastogenesis, HSV Blastogenesis, VZV Blastogenesis, Candida blastogenesis, ADV Blastogenesis, Tetanus Blastogenesis.

IOWA

Lymphocyte Proliferation to Mitogens. 3H Thymidine incorporation assay measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Concanvalin A (CON A), Pokeweed mitogen (PWM), Interleukin 2 (IL2) and anti-CD3.

Test code(s): SPL3

Lymphocyte Proliferation to Antigens. 3H Thymidine incorporation assay measuring lymphocyte proliferation to the antigens Tetanus/Diphtheria, Candida albicans and alloantigen.

Test code(s): SPL4

MAYO-ML

CMV-specific CD8 T cell immune competence is evaluated using MHC class I tetramers (flow assay also includes a quantitative component for CMV-specific CD8 T cells for 5 HLA alleles – A1, A2, B7, B8 and B35). Functional component includes CD107a/b expression and IFN-g production of CMV-specific CD8 T cells.

Test code(s): CMVC8; #88826

CD8 T cell degranulation (CD107a) and measurement of IFN-g after CD8 T cell activation with PMA/ionomycin.

Test code(s): GLIC; #89317

Flow based methodology measuring lymphocyte proliferation to an anti-CD3 panel (soluble anti-CD3, aCD3+aCD28, aCD3+ IL-2).

Test code(s): LPA3P; #62205

Flow based methodology measuring lymphocyte proliferation to the antigens Candida albicans and tetanus toxoid.

Test code(s): LPAG; #60592

Flow based methodology measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA) and Pokeweed (PWM).

Test code(s): LPMG; #60591

MCW-CIRL

CFSE-labeled T cells are assessed for their ability to proliferate to cytokines: anti-CD3, anti-CD3/IL-2, anti-CD3/IL-7, anti-CD3/IL-15, anti-CD3/anti-CD28.

Test code(s): TINTL

Flow-based methodology. CFSE-labeled T cells are assessed for their ability to proliferate to mitogens: ConA/IL-2, PHA/IL-2, and anti-CD3 with and without IL-2. Standard screening test for T cell function.

Test code(s): TMITO

MONTREAL-SJH

Degranulation CD3+CD8+ blasts (cytotoxic CD8 T lymphocytes) by flow cytometry.

Test code(s): Dégranulation des lymphocytes T

T cell proliferation after PHA and anti-CD3 (OKT3) antibody stimulation by flow cytometry.

Test code(s): PHA, OKT3

NJH-ADL

3H Thymidine incorporation in response to tetanus toxoid (3 concentrations) and Candida.

Test code(s): LCAND, LTET

3H Thymidine incorporation assay to measure lymphocyte proliferation to Phytohemagglutinin (PHA), Concanvalin A (Con A), and Pokeweed (PWM) (3 concentrations each). Available separately with

Test code(s): LPHA, LCONA, LPMW, or together:

NSUH

Lymphocyte proliferation to Antigen uses 3H thymidine incorporation method to measure lymphocyte proliferation to Tetanus toxoid and Candida albicans.

Test code(s): Antigen

Lymphocyte proliferation to Mitogen uses 3H thymidine incorporation method to measure lymphocyte proliferation to PHA, ConA, and PWM.

Test code(s): Mitogen

QUEST

Candida-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Candida.

Test code(s): 17270

Tetanus-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Tetanus Toxoid.

Test code(s): 17274

Tuberculin PPD-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Tuberculin PPD (TB PPD).

Test code(s): 17680

Mitogen-induced Lymphocyte Proliferation Panel. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Phytohemagglutinin (PHA), Concanavalin A (Con A), Pokeweed Mitogen (PWM). Test available by prior arrangement only.

Test code(s): 91976

Antigen-induced Lymphocyte Proliferation Panel (Candida, Tetanus, TB PPD). Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Candida, Tetanus Toxoid, Tuberculin PPD (TB PPD). Test available by prior arrangement only.

Test code(s): 91977

Mitogen- and Antigen-induced Lymphocyte Proliferation Panel. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Phytohemagglutinin (PHA), Concanavalin A (Con A), Pokeweed Mitogen (PWM), Candida, Tetanus Toxoid, Tuberculin PPD (TB PPD). Test available by prior arrangement only.

Test code(s): 91978

Phytohemagglutinin (PHA)-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Phytohemagglutinin (PHA). Test available by prior arrangement only.

Test code(s): 91980

Pokeweed Mitogen (PWM)-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Pokeweed Mitogen (PWM). Test available by prior arrangement only.

Test code(s): 91981

Concanavalin A (Con A)-induced Lymphocyte Proliferation. Cell culture and scintillation counter assay which measures lymphocyte proliferation in response to Concanavalin A (Con A). Test available by prior arrangement only.

Test code(s): 91982

UCLA-UIC

Flow-based methodology measuring lymphocyte proliferation to the mitogen Phytohemagglutinin (PHA) and to the antigens Candida Albicans and Tetanus Toxoid.

Test code(s): LPA-1M2A

Flow-based methodology measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Pokeweed (PWM) and Concanavalin A (Con A).

Test code(s): LPA-3M

Flow-based methodology measuring lymphocyte proliferation to the mitogens Phytohemagglutinin (PHA), Pokeweed (PWM) and Concanavalin A (Con A), and to the antigens Candida Albicans and Tetanus Toxoid.

Test code(s): LPA-3M2A

VIRACOR

T cell Function Analysis: PBMC isolation from whole blood, stimulation with various antigens or mitogens, flow cytometry analysis for CD69 upregulation and interferon gamma production.

Test code(s): 403066 and 403065

Intracellular Cytokine Panel: Whole blood is stimulated with PMA and ionomycin. Following stimulation cells are stained with antibodies to identify the cell population (CD4, CD8, NK and NKT cells) and cytokine (interferon gamma, IL4, or TNF) of interest.

Test code(s): 404474

NK CELL FUNCTIONAL TESTS

CALGARY-CLS

Evaluation of patient NK cell CD107a surface expression following exposure to K562 cells.

Test code(s): CD107A

CCHMC-DIL

Evaluation of CD107a surface expression/degranulation following exposure of NK cells to K562 cells.

Test code(s): CD107a Mobilization (NK Cell Degran)

Spontaneous NK cell cytotoxicity against K562 cells (4h stimulation, Cr51 assay).

Test code(s): NK Function

IOWA

Spontaneous NK cell cytotoxicity against K562 cells (4h stimulation, Cr51 assay).

Test code(s): SPL1

Enhanced NK cell cytotoxicity with Interleukin 2 stimulation against K562 cells (4h stimulation, Cr51 assay).

Test code(s): SPL2

MCW-CIRL

Flow cytometry assay using CFSE-labeled K562 target cells and 7-AAD incorporation by apoptotic target cells following culture with PBMC’s at various Effector:Target ratios. Test includes evaluation of CD107a surface expression/degranulation following exposure of NK cells to K562 cells.

Test code(s): CYTAPO

MONTREAL-SJH

Degranulation of NK cells by flow cytometry.

Test code(s): Dégranulation de NK

NSUH

NK function test measures CD107a surface expression after exposing NK cells to K562 cells.

Test code(s): NK function

QUEST

Natural Killer Cells, Functional. PBMC isolation from whole blood, incubation with fluorescently labeled K562 at various E:T target ratios, following incubation percent lysis is determined by flow cytometry.

Test code(s): 34184

UCLA-UIC

Flow cytometry assay using CFSE-labeled K562 target cells and Sytox Red incorporation by apoptotic target cells following culture with PBMCs at various Effector:Target ratios.

Test code(s): NKCC

VIRACOR

BMC isolation from whole blood, incubation with K562 (fluorescently labeled) at various E:T target ratios, following incubation percent lysis is determined by flow cytometry.

Test code(s): 30035

NEUTROPHIL FUNCTIONAL TESTS

Bactericidal Assays and Chemotaxis

NJH-ADL

Bactericidal assay. Analysis of the ability of isolated neutrophils to clear S. aureus – manual method.

Test code(s): BACT

Neutrophil chemotaxis. Modified Boyden chamber method utilizing peripheral blood and zymosan activated serum as chemotactic agent. Readout is distance migrated in microns.

Test code(s): CTX

DHR/Oxidative Burst

ARUP

Test code(s): 0096657

BCH-CFCL

Neutrophil’s Oxidative Burst, which reports: Neutrophil Oxidative Index (MFI DHR+ / MFI DHR-), and % Responding Polymorphonuclear Cells.

Test code(s): DHR assay

CALGARY-CLS

Functional CGD screening assay, using DHR123 loaded neutrophils pre and post stimulation with PMA to induce oxidative burst reaction.

Test code(s): NFUN

Functional CGD screening assay, using DHR123 loaded neutrophils pre and post stimulation with PMA to induce oxidative burst reaction.

Test code(s): NFUN

CCHMC-DIL

Classic dihydrorhodamine (DHR) 123 assay.

Test code(s): Neutrophil Oxidative Burst (DHR)

CHLA-DIFL

Chronic granulomatous disease diagnostic screening assay for oxidative burst using dihydrorhodamine (DHR) 123.

Test code(s): CGD Flow

IOWA

Classic dihydrorhodamine (DHR) 123 assay with PMA stimulation.

Test code(s): SPL8

MAYO-ML

Classic dihydrorhodamine (DHR) 123 assay.

Test code(s): DHR (for both PMA and fMLP stimulants); #62764; DHRP (only for PMA); #62765; DHRF (only for fMLP); #62766.

MONTREAL-SJH

Neutrophil’s oxidation (DHR) by flow cytometry.

Test code(s): Activité Oxidative des Neutrophiles

NJH-ADL

Classic dihydrorhodamine (DHR) 123 assay. Offers NBT assay as back up.

Test code(s): DHR; NBT

NSUH

Neutrophil oxidation assay uses dihydrorhodamine (DHR) 123 and PMA. Report includes MFI ratio.

Test code(s): DHR

QUEST

Neutrophil Function, Oxidative Burst. Classic dihydrorhodamine (DHR) 123 assay.

Test code(s): 37523

UCLA-UIC

Classic dihydrorhodamine (DHR) 123 assay with PMA stimulation.

Test code(s): GOB

VIRACOR

Test code(s): 403002

CYTOKINE PANELS, INDIVIDUAL CYTOKINES AND SOLUBLE PROTEINS

Cytokine Panels

ARUP

Cytokine Panel. Reported: Interleukin 2 receptor (CD25) soluble; Interferon gamma; Interleukin-1beta, 2, 4, 5, 6, 8, 10, 12, 13, 17; TNF-alpha. Quantitative Multiplex Bead Assay.

Test code(s): 0051394

Cytokine Panel, TH1. Reported: Interleukin 2, 12; Interleukin 2 receptor (CD25) soluble; Interferon gamma. Quantitative Multiplex Bead Assay.

Test code(s): 0051408

Cytokine Panel, TH2. Reported: Interleukin 4, 5, 10, 13. Quantitative Multiplex Bead Assay.

Test code(s): 0051518

Cytokine Panel, Monokines. Reported: Interleukin 1beta, 6, 8, TNF alpha. Quantitative Multiplex Bead Assay.

Test code(s): 0051524

CCHMC-DIL

Plasma Cytokine Panel. Luminex Multiplex Panel includes IL-1beta, 2, 4, 5, 6, 8, 10; Interferon-gamma; TNF-alpha; GM-CSF.

Test code(s): Cytokines, Plasma or CSF

NJH-ADL

TH1 Cytokine 4 Plex Panel: Interleukin 2 [IL-2], Interleukin 12 [IL-12p70], Interferon Gamma [IFNg], Tumor Necrosis Factor alpha [TNFa]. Quantitative Multiplex Bead Assay.

Test code(s): PLASMA TH1

TH2 Cytokine 4 Plex Panel: Interleukin 4 [IL-4], Interleukin 5 [IL-5], Interleukin 6 [IL-6], Interleukin 10 [IL-10]. Quantitative Multiplex Bead Assay.

Test code(s): PLASMA TH2P

TH1/TH2 Panel A: Interleukin 2 [IL-2], Interleukin 4 [IL-4], Interleukin 5 [IL-5], Interleukin 6 [IL-6], Interleukin 10 [IL-10], Interleukin 12 [IL-12p70], Interferon Gamma [IFNg], Tumor Necrosis Factor alpha [TNFa]. Quantitative Multiplex Bead Assay.

Test code(s): PLASMA THPNLA

TH1/TH2 Panel B: Interleukin 2 [IL-2], Interleukin 4 [IL-4], Interleukin 5 [IL-5], Interleukin 10 [IL-10], Interferon Gamma [IFNg], Tumor Necrosis Factor alpha [TNFa]. Quantitative Multiplex Bead Assay.

Test code(s): PLASMA THPNLB

VIRACOR

Th1/Th2 Panel Includes: Interferon-gamma, Interleukin-13, Interleukin-12p70, Interleukin-10, Interleukin-5, Interleukin-4, and Interleukin-2. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.

Test code(s): 403025 Serum or 403026 Plasma

Pro-inflammatory Panel Includes: Interleukin-1b, Interleukin-6, Interleukin-8, and TNF-alpha. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.

Test code(s): 403027 Serum or 403028 Plasma

Individual Cytokines and Soluble Proteins

CCHMC-DIL

CXCL9.

Test code(s): CXCL9

S100A12.

Test code(s): S100A12

S100A8/A9 Heterodimer

ARUP

Interleukin 5. Multiplex Bead Assay.

Test code(s): 0051533

VIRACOR

Interleukin-5. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.

Test code(s): 30042 Serum or 1228 Plasma

Interleukin-6

ARUP

Interleukin 6. Multiplex Bead Assay.

Test code(s): 0051537

QUEST

Interleukin 6. Measured by high sensitivity ELISA.

Test code(s): 34473X

VIRACOR

Interleukin-6. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.

Test code(s): 1257 Serum or 1258 Plasma

Interleukin-8

ARUP

Interleukin 8. Multiplex Bead Assay.

Test code(s): 0051538

VIRACOR

Interleukin-8. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.

Test code(s): 1219 Serum or 1229 Plasma

Neopterin

CCHMC-DIL

Neopterin.

Test code(s): Neopterin, Plasma or CSF

Soluble CD163

CCHMC-DIL

Soluble CD163.

Test code(s): Soluble CD163

Soluble Fas Ligand

CCHMC-DIL

Soluble Fas Ligand.

Test code(s): Soluble Fas-Ligand (sFasL)

MONTREAL-SJH

ALPS- Plasma FAS Ligand/CD95 (ELISA).

Test code(s): Dosage du FAS LIGAND

Soluble IL2R (CD25)

ARUP

Interleukin 2 Receptor (CD25), Soluble. By Quantitative Multiplex Bead Assay.

Test code(s): 0051529

CCHMC-DIL

2 Receptor (CD25), Soluble.

Test code(s): Soluble IL-2R (Soluble CD25)

QUEST

Interleukin-2 Receptor EIA. Soluble IL2R levels measured by EIA.

Test code(s): 34298

VIRACOR

sIl-2R. The assay employs a sandwich immunoassay format.

Test code(s): 30057

Tumor Necrosis Factor – alpha

ARUP

Tumor Necrosis Factor – alpha. Multiplex Bead Assay.

Test code(s): 0051539

VIRACOR

TNF-alpha. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection.

Test code(s): 1220 Serum or 1230 Plasma

COMPLEMENT TESTS (INCLUDING INDIVIDUAL COMPONENTS, FUNCTIONAL ASSAYS, AND SPLIT PRODUCTS) - GROUPED BY SPECIALTY LAB

MAYO-ML

Complement, Alternative Pathway (AH50), Functional Serum (separately orderable).

Test code(s): AH50; #88676

Atypical Hemolytic Uremic Syndrome (aHUS) Complement Panel.

Test code(s): AHUSD; #64881

C1 Esterase (C1ES) Inhibitor Antigen, Serum (nephelometry).

Test code(s): C1ES; #8198

Complement C1q, Serum (nephelometry).

Test code(s): C1Q; #8851

C1q Complement Functional, Serum (Automated Liposome Lysis [ALL]).

Test code(s): C1QFX; #83374

C2 Complement Functional, Serum (ALL).

Test code(s): C2; #81835

C2 Complement Antigen, Serum (RID – reflex testing only).

Test code(s): C2AG; #84141

C2 Complement Functional (no reflex, ALL).

Test code(s): C2FXN; #32137

Complement C3, Serum (nephelometry).

Test code(s): C3; #8174

C3 Functional, Serum (ALL).

Test code(s): C3FX; #81090

Complement C4, Serum (nephelometry).

Test code(s): C4; #88171

C4d Complement Activation Fragment, Plasma (AHUSD only).

Test code(s): C4D; #62584

C4 Functional, Serum (ALL).

Test code(s): C4FX; #83391

C5 Complement, Antigen, Serum (nephelometry).

Test code(s): C5AG; #9266

C5 Functional, Serum (ALL).

Test code(s): C5FX; #83392

C6 Functional, Serum (ALL

Test code(s): C6FX; #83393

C7 Functional, Serum (ALL).

Test code(s): C7FX; #81064

C8 Functional, Serum (ALL).

Test code(s): C8FX; #81065

C9 Functional, Serum (ALL).

Test code(s): C9FX; #81066

CBB Complement Activation Fragment, Plasma (AHUSD only).

Test code(s): CBB; #62585

Complement, Total, Serum.

Test code(s): COM; #8167

Factor B Complement Antigen (AHUSD only, nephelometry).

Test code(s): FBCA; #62588

C1 Esterase Inhibitor Functional Assay, Serum (ELISA).

Test code(s): FC1EQ; #81493

Factor H Complement Antigen (AHUSD only, nephelometry).

Test code(s): FHCA; #62587

SC5b-9 Complement Activation Complex, Plasma (AHUSD only).

Test code(s): SC5B9; # 62586

VIRACOR

C1 Esterase Inhibitor Functional.

Test code(s): 1112

C1 Esterase Inhibitor Protein.

Test code(s): 1113

C1q Complement.

Test code(s): 1115

Mannan Binding Lectin (MBL).

Test code(s): 33

Mannan-Binding Lectin Pathway Function Test (C4b).

Test code(s): 34

C4 Complement.

Test code(s): 8

Autoantibodies to Individual Components

ARUP

Immune Complex Panel (by Flow and ELISA).

Test code(s): 0050667

NJH-ADL

Autoantibody to C1q (C1q-CLR, by ELISA).

Test code(s): C1QAB

C3 Nephritic factor (by Immunofixation Electrophoresis).

Test code(s): C3NEF

Autoantibody to C1-inhibitor by (ELISA).

Test code(s): CEIAP

Autoantibody to Factor H (by ELISA).

Test code(s): FHAB

Component Levels

ARUP

C1-Esterase Inhibitor Panel (by ELISA, Turb and Neph).

Test code(s): 0050139

C1-Esterase Inhibitory (by Quant Neph).

Test code(s): 0050140

Complement Component 2 (by RID).

Test code(s): 0050148

Complement Components 3 and 4 (by Quant. Immunoturbid).

Test code(s): 0050149

Complement Component 3 (by Quant. Immunoturbidimetry).

Test code(s): 0050150

Complement Component 4 (by Quant. Immunoturbidimetry).

Test code(s): 0050155

Complement Component 5 (by RID).

Test code(s): 0050156

Complement Factor B (by RID).

Test code(s): 0051720

Complement Component 1Q Level (by RID).

Test code(s): 0099130

NJH-ADL

C1q level (by RID).

Test code(s): C1Q

C1r level (by RID).

Test code(s): C1RL

C1s level (by RID).

Test code(s): C1SL

C2 level (by RID).

Test code(s): C2L

C3 level (by nephelometry).

Test code(s): C3

C4 level (by nephelometry).

Test code(s): C4

C5 level (by RID).

Test code(s): C5L

C6 level (by RID).

Test code(s): C6L

C7 level (by RID).

Test code(s): C7L

C8 level (by RID).

Test code(s): C8L

C9 level (by RID).

Test code(s): C9L

C1-esterase inhibitor level (C1-INH, by RID).

Test code(s): CEIQ

Circulating immune complexes (C1q-binding and C3d).

Test code(s): CIC

Factor B level (by RID).

Test code(s): FBL

Factor D level (by ELISA).

Test code(s): FDL

Factor H level (by RID).

Test code(s): FH

Factor I level (by RID).

Test code(s): FIL

Mannose binding lectin (by ELISA).

Test code(s): MLEC

Properdin level (by ELISA).

Test code(s): PROP

QUEST

Mannose binding lectin (by ELISA).

Test code(s): 16039X

C1-esterase inhibitor protein (quantitative, by EIA).

Test code(s): 298

Properdin Factor B (by nephelometry).

Test code(s): 300

C7 level (by RID).

Test code(s): 34888X

C8 level (by RID).

Test code(s): 34889X

C9 level (by RID).

Test code(s): 34896

C3 level (by nephelometry).

Test code(s): 351

C4 level (by nephelometry).

Test code(s): 353

C5 level (by RID).

Test code(s): 354

Circulating immune complexes (C1q-binding, by ELISA).

Test code(s): 36735

C2 level (by RID).

Test code(s): 433X

C6 level (by RID).

Test code(s): 464

C1q level (by RID).

Test code(s): 981X

Functional Assays

ARUP

C1-Esterase Inhibitor Functional (by ELISA).

Test code(s): 0050141

Complement Activity Enzyme Immunoassay, Total (by ELISA).

Test code(s): 0050198

C1q Binding Assay (by ELISA).

Test code(s): 0050301

Complement Activity, Alternative Pathway (AH50, by RID).

Test code(s): 2005373

NJH-ADL

C1 function (by hemolytic assay).

Test code(s): C1F

C1q function (by hemolytic assay).

Test code(s): C1QH

C2 function (by hemolytic assay).

Test code(s): C2F

C3 function (by hemolytic assay).

Test code(s): C3F

C4 function (by hemolytic assay).

Test code(s): C4F

Rapid screen for deficiency of late components (C5, C6, C7, C8, C9, CH50).

Test code(s): C59S

C5 function (by hemolytic assay).

Test code(s): C5F

C6 function (by hemolytic assay).

Test code(s): C6F

C7 function (by hemolytic assay).

Test code(s): C7F

C8 function (by hemolytic assay).

Test code(s): C8F

C9 function (by hemolytic assay).

Test code(s): C9F

C1 esterase inhibitor function, Chromogenic.

Test code(s): CEICHR

Factor D function (by hemolytic assay).

Test code(s): FDF

Factor B function (by hemolytic assay).

Test code(s): PFBF

QUEST

C1-esterase inhibitor protein (functional, by EIA).

Test code(s): 297

Individual Complement Split Products

NJH-ADL

Bb level (by ELISA).

Test code(s): BBLL

C3a desArg level (by RIA).

Test code(s): C3AL

C4a desArg level (by RIA).

Test code(s): C4AL

C4d level (by ELISA).

Test code(s): C4D

Ratio of C4d to C4.

Test code(s): C4RAT

C5a desArg level (by RIA).

Test code(s): C5AL

iC3b level (by ELISA).

Test code(s): IC3B

SC5b-9 level (by ELISA).

Test code(s): SC5B9

QUEST

C3a desarg (by ELISA).

Test code(s): 17689X

C3d fragment (by EIA).

Test code(s): 4943N

OTHER SPECIALIZED TESTS

Autoantibodies to Cytokines: Detection and Functional Assays

Autoantibodies to GMCSF

CCHMC-DIL

Measurement of STAT5 phosphorylation following GMCSF stimulation.

Test code(s): GM-CSF Receptor Stimulation

Measurement of autoantibodies to GMCSF by ELISA.

Test code(s): GMCSF Autoantibody

MONTREAL-SJH

Measures of phosphorylation/dephosphorylation STAT5 (IL2, IL3, IL15, and GMCSF-induced) by flow cytometry.

Test code(s): Étude functionnelle and indicate yourself

NJH-ADL

ELISA measurement of GM-CSF autoantibodies, and reflex testing of P-STAT5 inhibition for positive samples.

Test code(s): GMCSFA

Autoantibodies to Interferon Gamma

MONTREAL-SJH

Measures of phosphorylation of STAT1 in response to gIFN by flow cytometry.

Test code(s): Étude functionnelle and indicate yourself

NJH-ADL

Binding and functional analysis of autoantibodies to IFNg by ELISA and reflex P-STAT1 inhibition assay.

Test code(s): IFNGAB

Molecular Tests (non-Gene Sequencing)

TCR Vb Spectratyping for T Cell Receptor Repertoire Diversity

MAYO-ML

Both visual interpretation of spectratype and diversity ratio information included in report.

Test code(s): TCRVB; #62930

QUEST

T-cell Receptor (TCR) Gamma Gene Rearrangement, PCR. Interrogates the T-cell receptor gamma locus (TCRG) by a PCR method based on the BIOMED-2 consensus.

Test code(s): 90509

T-cell Clonality Panel (TCRG, TCRB), PCR. Interrogates both the T-cell receptor beta (TCRB) locus and the T-cell receptor gamma (TCRG) locus by a PCR method based on the BIOMED-2 consensus.

Test code(s): 91445

T-cell Receptor (TCR) Beta Gene Rearrangement, PCR. Interrogates the T-cell receptor beta locus (TCRB) by a PCR method based on the BIOMED-2 consensus.

Test code(s): 91446

TREC Quantitation Relative to CD3 T Cell Count

MAYO-ML

TREC quantitatoin reported as copies per million CD3+ T cells.

Test code(s): TREC; #87959

Specialized Serologic Testing for Vaccine Response Evaluation (not including routine vaccine titers)

Functional Antibody Analysis

VIRACOR

Functional Antibody Analysis- Pneumococcal Avidity. A modification of the standard pneumococcal assay, using varying concentrations of ammonium thiocyanate. Bound antibodies are detected with an antibody that recognizes human IgG. The IgG antibody concentration is calculated using a standard curve referenced to WHO standard serum 89SF. The avidity is calculated based on binding with various concentrations of thiocyanate. Multiple Panels available with varying number of serotypes:

Test code(s): 401734, 401846, 401839, and 4017345

Therapeutic Monitoring Lymphocyte Depletion

Therapeutic Monitoring Following Alemtuzumab (Alemtuzumab Levels)

CCHMC-DIL

Flow based assay to measure alemtuzumab levels in whole blood.

Test code(s): Alemtuzumab Level

Therapeutic Monitoring Following Alemtuzumab (CD52 Expression)

CCHMC-DIL

Quantifies CD52 on T cells, NK cells, B cells, monocytes.

Test code(s): CD52 Expression